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1.1.3.10: pyranose oxidase

This is an abbreviated version!
For detailed information about pyranose oxidase, go to the full flat file.

Word Map on EC 1.1.3.10

Reaction

D-glucose
+
O2
=
2-dehydro-D-glucose
 +
H2O2

Synonyms

C-2 specific pyranose-2-oxidase, carbohydrate oxidase, glucose 2-oxidase, glucose-2-oxidase, P2O, P2Ox, POX, PROD, PyOx, pyranose 2-Oxidase, pyranose oxidase, pyranose-2-oxidase, pyranose/oxygen 2-oxidoreductase, pyranose: oxygen 2-oxidoreductase, pyranose:oxygen 2-oxidoreductase, pyranose:oxygen-2-oxidoreductase, TmP2Ox

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.10 pyranose oxidase

Storage Stability

Storage Stability on EC 1.1.3.10 - pyranose oxidase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 0.1 M Tris-HCl, pH 7.0, enzyme retains 78% of the initial activity after 6 months in absence of glycerol
-
-20°C, 50 mM Tris-HCl, pH 8, 30% glycerol, more than 1 year
-
23°C, at moderate pressures and atmospheric pressure conditions, 3 days, no significant change in enzyme activity
-
25°C, half-life of native enzyme is 5 weeks, half-life of immobilized enzyme is 11 weeks
40°C, 10 mM sodium phosphate, pH 6-8, half life of enzyme activity: more than 300 days
-
4°C, 30 mM sodium phosphate buffer, pH 6.5, 3 weeks
-
4°C, alpha-glucosidase/pyranose oxidase/chitosan and alpha-glucosidase/pyranose oxidase/chitosan-carbon nanotube biosensors, 20 days, no loss of activity
Coriolus sp.
-
4°C, over anhydrous silica gel, 1 year
Polyporus obtusus
-
4°C, pH 4-11, retains more than 85% of the residual activity, 1 week
-
4°C, pH 7.0, enzyme retains more than 90% of the activity
-
50°C, 10 mM sodium phosphate, pH 7, half life of enzyme activity: more than 110 days
-
65°C, 10 mM sodium phosphate, pH 7, half life of enzyme activity: 1 h
-
inactivated by freezing and thawing
-
repeated freezing at -20°C and thawing results in a rapid and irreversible loss of activity
room temperature and 30°C, pH 5-10, half life of enzyme activity: more than 300 days
-