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1.1.1.B20: meso-2,3-butandiol dehydrogenase

This is an abbreviated version!
For detailed information about meso-2,3-butandiol dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.B20

Reaction

(2R,3S)-butane-2,3-diol
+
NAD+
=
acetoin
 +
NADH
 +
H+

Synonyms

(2R,3R)-2,3-butanediol dehydrogenase, 2,3-BD dehydrogenase, 2,3-butanediol dehydrogenase, 2,3-butanediol dehydrogenases, ADH-9, ARA1, BDH, BdhA, BS-BDH, BtBDH, budC, butACg, butanediol dehydrogenase, ButB, CG-BDH, Cgl2674, mbdh, meso-2,3-BD dehydrogenase, meso-2,3-BDH, meso-2,3-butanediol dehydrogenase, meso-acetoin reductase, meso-BD, meso-BDH, MF996569, More, NAD(H)-dependent meso-2,3-BDH, NAD(H)-dependent meso-2,3-butanediol dehydrogenase, PA4153, PB24_3312, PF-BDH, PF1960, PT-BDH, R,R-2,3-butanediol dehydrogenase/meso-2,3-butanediol dehydrogenase/diacetyl reductase, SmBdh

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.B20 meso-2,3-butandiol dehydrogenase

Cloned

Cloned on EC 1.1.1.B20 - meso-2,3-butandiol dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
ADH-9 gene is identified from a biodiversity library, recombinant expression in Escherichia coli strain BL21/(DE3)
-
DNA and amino acid sequence deterination and analysis, sequence comparisons and phylogenetic analysis
expression in Escherichia coli
-
gene bdh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Escherichia coli
gene bdh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli
gene bdh1, phylogenetic analysis, expression in Escherichia coi strain YYC202(DE3) ldhA-/- ilvC? expressing ilvBN from Escherichia coli and aldB from Lactobacillus lactis, encoding acetolactate synthase and acetolactate decarboxylase activities, respectively, disruption of the lactate biosynthesis pathway in the strain increases pyruvate precursor availability to this strain, increased availability of NADH for acetoin reduction to meso-2,3-butanediol is the most important consequence of ldhA deletion
-
gene bdhA, the the BDH gene sequence is cloned from genomic DNA from strain 168, the BDH encoded by the gene bdhA is responsible for catalysing the reaction from (3R)-acetoin to (2R,3R)-2,3-butanediol
gene budC , recombinant expression in Escherichia coli strain BL21(DE3)
gene budC, expression in Escherichia coli strain BL21(DE3) pLys
-
gene budC, phylogenetic tree, functional recombinant expression in Bacillus subtilis strain 168
-
gene budC, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21 Star (DE3)
gene budC, recombinant expression of SUMO-His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21-Gold from plasmid pET28a-SUMO-KpBDH
gene budC, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
gene budC, sequence comparisons and phylogenetic analysis, recombinant expression in Zymomonas mobilis. Crude extracts from Zymomonas mobilis expressing Serratia marcescens Bdh (SmBdh) have the highest activity (8.89 micromol/min/mg), when compared to other Bdh enzymes (0.34-2.87 micromol/min/mg), overview
gene budC, sequence comparisons, expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
gene butACg, subcloning in Escherichia coli strain DH5alpha, recombinant expression in Escherichia coli strain BL21(DE3), coexpression with the genes encoding ALS and ALDC of Lactobacillus lactis strain MG1363
-
gene mbdh, functional coexpression of Serratia sp. meso-2,3-butanediol dehydrogenase, Lactobacillus brevis NADH oxidase and Vitreoscilla sp. hemoglobin in Escherichia coli strain BL21(DE3)/pET-mbdh-nox-vgb, construction of plasmids pET-mbdh, pET-mbdh-nox, and pET-mbdh-nox-vgb, method optimization
AEF50077
gene mbdh, recombinant expression of meso-2,3-BDH in Escherichia coli strain BL21(DE3), coexpression with NADH oxidase (NOX) from Lactobacillus brevis, and hemoglobin protein (VHB) from Vitreoscilla, subcloning in Escherichia coli strain DH5alpha
-
genetic structure of the 2,3-BD utilization operon in Pseudomonas aeruginosa strain PAO1 and comparisons, overview
phylogenetic analysis, enzyme expression in Escherichia coli strains MG1655(DE3) and YYC202(DE3)
-
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)