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0.088 - 53.1
2-oxoglutarate
0.00025 - 0.0054
3-acetylpyridine-NAD+
0.00016
3-acetylpyridine-NADH
-
25°C
0.003 - 320
3-phospho-D-glycerate
0.212
3-phosphoglycerate
pH 9.0
0.00013 - 40.2
3-phosphohydroxypyruvate
0.0032 - 0.17
3-phosphooxypyruvate
0.0393
acetylpyridine
pH 7.5, temperature not specified in the publication
-
0.038 - 0.088
alpha-ketoglutarate
0.4
D-2-hydroxyglutarate
pH and temperature not specified in the publication
0.15 - 1.1
D-3-phosphoglycerate
0.015
phosphohydroxypyruvate
pH 6.5
0.1477
pyridinealdehyde adenine dinucleotide
pH 7.5, temperature not specified in the publication
-
0.0118
Thionicotinamide
pH 7.5, temperature not specified in the publication
additional information
additional information
-
0.088
2-oxoglutarate
pH and temperature not specified in the publication
0.45
2-oxoglutarate
wild-type enzyme, pH 7.0, temperature not specified in the publication
3.7
2-oxoglutarate
pH 6.0, 25°C
5
2-oxoglutarate
mutant enzyme K141R, pH 7.0, temperature not specified in the publication
7.6
2-oxoglutarate
pH 6.0, 25°C
10.1
2-oxoglutarate
-
at pH 7.6 and 37°C
10.7
2-oxoglutarate
pH 6.0, 25°C
30.6
2-oxoglutarate
mutant enzyme R72L, pH 7.0, temperature not specified in the publication
53.1
2-oxoglutarate
mutant enzyme R72A, pH 7.0, temperature not specified in the publication
0.00025
3-acetylpyridine-NAD+
-
25°C
0.0054
3-acetylpyridine-NAD+
-
37°C
0.003
3-phospho-D-glycerate
-
in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication
0.004
3-phospho-D-glycerate
-
in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication
0.008
3-phospho-D-glycerate
-
in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication
0.014
3-phospho-D-glycerate
in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication
0.015
3-phospho-D-glycerate
-
in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication
0.025
3-phospho-D-glycerate
-
in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication
0.031
3-phospho-D-glycerate
-
in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication
0.033
3-phospho-D-glycerate
-
value for 3-phospho-D-glycerate oxidation using NAD+ as a cofactor
0.041
3-phospho-D-glycerate
-
in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication
0.049
3-phospho-D-glycerate
-
in 50 mM MOPS buffer, pH 7.0, temperature not specified in the publication
0.05
3-phospho-D-glycerate
-
-
0.05
3-phospho-D-glycerate
pH 8.0, 22°C
0.065
3-phospho-D-glycerate
-
in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication
0.127
3-phospho-D-glycerate
-
in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication
0.153
3-phospho-D-glycerate
-
in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication
0.162
3-phospho-D-glycerate
in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication
0.187
3-phospho-D-glycerate
pH 7.5, temperature not specified in the publication
0.19
3-phospho-D-glycerate
pH 7.5, mutant G336V
0.23
3-phospho-D-glycerate
pH 7.5, mutant H344A
0.26
3-phospho-D-glycerate
pH 7.5, mutant A144V
0.26
3-phospho-D-glycerate
-
at pH 7.6 and 37°C
0.28
3-phospho-D-glycerate
pH 7.5, mutant E387A
0.29
3-phospho-D-glycerate
pH 7.5, mutant N364A
0.292
3-phospho-D-glycerate
-
in 200 mM KPO4 buffer, pH 7.0, temperature not specified in the publication
0.294
3-phospho-D-glycerate
pH 8.5, temperature not specified in the publication
0.3
3-phospho-D-glycerate
pH 7.5, mutant E302A
0.33
3-phospho-D-glycerate
pH 7.5, mutant R338A
0.35
3-phospho-D-glycerate
pH 7.5, mutants A374V and D386A
0.39
3-phospho-D-glycerate
pH 7.5, mutant N346A
0.396
3-phospho-D-glycerate
wild type enzyme, at pH 9.0 and 25°C
0.45
3-phospho-D-glycerate
pH 7.5, mutant R347A
0.47
3-phospho-D-glycerate
pH 7.5, mutant Q361A
0.49
3-phospho-D-glycerate
pH 7.5, wild-type enzyme and mutant Q375A
0.49
3-phospho-D-glycerate
pH 9.0, temperature not specified in the publication
0.5
3-phospho-D-glycerate
pH 9.0, 25°C
0.54
3-phospho-D-glycerate
pH and temperature not specified in the publication
0.58
3-phospho-D-glycerate
pH 7.5, mutant P348A
0.6
3-phospho-D-glycerate
mutant enzyme K263A, at pH 9.0 and 25°C
0.63
3-phospho-D-glycerate
pH 7.5, mutant S316A
0.64
3-phospho-D-glycerate
pH 7.5, mutant G337V
0.66
3-phospho-D-glycerate
pH 7.5, mutant E360A
0.68
3-phospho-D-glycerate
pH 7.5, mutants S323A and G362V
0.69
3-phospho-D-glycerate
pH 7.5, mutant G349V
0.72
3-phospho-D-glycerate
pH 7.5, mutant S373A
0.76
3-phospho-D-glycerate
pH 7.5, mutant E345A
0.77
3-phospho-D-glycerate
pH 9.0, 25°C
0.79
3-phospho-D-glycerate
pH 7.5, mutant H335A
0.899
3-phospho-D-glycerate
isoform PGDH2, at pH 8.1 and 37°C
0.9
3-phospho-D-glycerate
-
-
0.97
3-phospho-D-glycerate
pH 7.5, mutant S107A
1.006
3-phospho-D-glycerate
isoform PGDH3, at pH 8.1 and 37°C
1.03
3-phospho-D-glycerate
pH 9.0, 25°C
1.19
3-phospho-D-glycerate
-
pH 9.0
1.19
3-phospho-D-glycerate
pH 7.5, mutant A143V
1.2
3-phospho-D-glycerate
pH and temperature not specified in the publication
1.308
3-phospho-D-glycerate
isoform PGDH1, at pH 8.1 and 37°C
1.35
3-phospho-D-glycerate
-
-
1.37
3-phospho-D-glycerate
pH 7.5, mutant S296A
1.38
3-phospho-D-glycerate
30°C, pH 9.0
1.41
3-phospho-D-glycerate
pH 7.5, mutants Q298A and N303A
1.6
3-phospho-D-glycerate
pH 7.5, mutant D317A
1.77
3-phospho-D-glycerate
pH 7.5, mutant E307A
1.926
3-phospho-D-glycerate
isoform PGDH2, at pH 7.2 and 37°C
1.99
3-phospho-D-glycerate
pH 7.5, mutant E299A
2.11
3-phospho-D-glycerate
isoform PGDH1, at pH 7.2 and 37°C
2.559
3-phospho-D-glycerate
isoform PGDH3, at pH 7.2 and 37°C
2.92
3-phospho-D-glycerate
30°C, pH 9.0
4.33
3-phospho-D-glycerate
pH 7.5, mutant S111A
4.78
3-phospho-D-glycerate
pH 7.5, mutant Q301A
6.6
3-phospho-D-glycerate
pH 7.5, mutant S107A/S111A
7.2
3-phospho-D-glycerate
pH 7.5, mutant G145V
12
3-phospho-D-glycerate
pH 7.5, mutant K311A
13.1
3-phospho-D-glycerate
pH 7.5, mutant N303A/K311A
16.9
3-phospho-D-glycerate
pH 7.5, mutant T297A
29.1
3-phospho-D-glycerate
pH 7.5, mutant K141A
320
3-phospho-D-glycerate
pH 7.5, mutant S111A/K311A
0.00013
3-phosphohydroxypyruvate
-
25°C
0.0013
3-phosphohydroxypyruvate
-
Km at pH 7.5 is lower than at pH 8.8
0.0032
3-phosphohydroxypyruvate
-
apparent
0.0096
3-phosphohydroxypyruvate
-
value for 3-phosphohydroxypyruvate reduction using NADH as a cofactor
0.015 - 0.02
3-phosphohydroxypyruvate
pH 7.1, 25°C, wild-type enzyme from fibroblasts
0.0216
3-phosphohydroxypyruvate
pH 7.1, 25°C, recombinant FLAG-tagged wild-type enzyme from HEK-293 cells
0.045
3-phosphohydroxypyruvate
-
25°C
0.07
3-phosphohydroxypyruvate
-
37°C
0.075
3-phosphohydroxypyruvate
mutant K439A
0.085
3-phosphohydroxypyruvate
-
pH 7.5, 37°C
0.12
3-phosphohydroxypyruvate
-
value for 3-phosphohydroxypyruvate reduction using NADPH as a cofactor
0.123
3-phosphohydroxypyruvate
mutant R446A
0.15
3-phosphohydroxypyruvate
-
-
0.16
3-phosphohydroxypyruvate
mutant H447A
0.17
3-phosphohydroxypyruvate
-
0.18
3-phosphohydroxypyruvate
mutant R501A
0.19
3-phosphohydroxypyruvate
mutant R451A
0.243
3-phosphohydroxypyruvate
mutant R501A/R451A/K439A
0.35
3-phosphohydroxypyruvate
-
pH 7.1
40.2
3-phosphohydroxypyruvate
-
-
0.0032
3-phosphooxypyruvate
pH and temperature not specified in the publication
0.015
3-phosphooxypyruvate
pH 7.0, temperature not specified in the publication
0.015
3-phosphooxypyruvate
mutant enzyme R132K, pH 7.0, temperature not specified in the publication
0.027
3-phosphooxypyruvate
pH and temperature not specified in the publication
0.068
3-phosphooxypyruvate
wild-type enzyme, pH 7.0, temperature not specified in the publication
0.081
3-phosphooxypyruvate
mutant enzyme K141R, pH 7.0, temperature not specified in the publication
0.09355
3-phosphooxypyruvate
30°C, pH 7.5
0.1164
3-phosphooxypyruvate
30°C, pH 7.5
0.153
3-phosphooxypyruvate
wild-type enzyme, pH 7.0, temperature not specified in the publication
0.169
3-phosphooxypyruvate
mutant enzyme R72A/R132K, pH 7.0, temperature not specified in the publication
0.17
3-phosphooxypyruvate
pH and temperature not specified in the publication
0.038
alpha-ketoglutarate
-
apparent, double mutant H344A/N364A
0.042
alpha-ketoglutarate
-
apparent
0.044
alpha-ketoglutarate
-
apparent, mutant N346A
0.088
alpha-ketoglutarate
-
apparent
0.15
D-3-phosphoglycerate
-
pH 7.5
0.29
D-3-phosphoglycerate
-
pH 9.4
1.1
D-3-phosphoglycerate
-
-
1.1
D-3-phosphoglycerate
-
Km at pH 7.5 is lower than at pH 8.8
0.0053
NAD+
pH 7.5, temperature not specified in the publication
0.0078
NAD+
-
Km at pH 7.5 is lower than at pH 8.8
0.022
NAD+
-
with 3-phospho-D-glycerate as cosubstrate, at pH 7.6 and 37°C
0.027
NAD+
pH 7.0, temperature not specified in the publication
0.0304
NAD+
pH 8.5, temperature not specified in the publication
0.04
NAD+
-
value for 3-phospho-D-glycerate oxidation
0.096
NAD+
pH 9.0, temperature not specified in the publication
0.189
NAD+
isoform PGDH2, at pH 8.1 and 37°C
0.239
NAD+
isoform PGDH3, at pH 8.1 and 37°C
0.271
NAD+
isoform PGDH2, at pH 7.2 and 37°C
0.377
NAD+
isoform PGDH1, at pH 7.2 and 37°C
0.39
NAD+
isoform PGDH1, at pH 8.1 and 37°C
0.551
NAD+
isoform PGDH3, at pH 7.2 and 37°C
0.0005
NADH
-
25°C
0.0013
NADH
-
value for 3-phosphohydroxypyruvate reduction using NADH as a cofactor
0.004
NADH
-
with 2-oxoglutarate as cosubstrate, at pH 7.6 and 37°C
0.004
NADH
-
with oxaloacetate as cosubstrate, at pH 7.6 and 37°C
0.025
NADH
pH 7.0, temperature not specified in the publication
0.114
NADH
pH 7.5, 25°C, recombinant mutant G316V
0.12
NADH
pH 7.5, 25°C, recombinant mutant N481A
0.14
NADH
pH 7.5, 25°C, recombinant mutant D463A
0.165
NADH
pH 7.5, 25°C, recombinant mutant G317V
0.17
NADH
pH 7.5, 25°C, wild-type enzyme
0.203
NADH
pH 7.5, 25°C, recombinant mutant G318V
0.22
NADH
pH 7.5, 25°C, recombinant mutant G317V/G318V
0.27
NADH
pH 7.5, 25°C, recombinant mutant Y461A
0.47
NADH
pH 7.5, 25°C, recombinant mutant G316V/G318V
0.61
NADH
pH 7.5, 25°C, recombinant mutant G316V/G317V
0.02
NADPH
-
value for 3-phosphohydroxypyruvate reduction using NADPH as a cofactor
3.9
oxaloacetate
pH 6.0, 25°C
6.5
oxaloacetate
-
at pH 7.6 and 37°C
11.2
oxaloacetate
pH 6.0, 25°C
14.1
oxaloacetate
pH 6.0, 25°C
additional information
additional information
-
-
-
additional information
additional information
kinetics, overview
-
additional information
additional information
-
kinetics, overview
-
additional information
additional information
kinetic analysis of wild-type and mutant enzymes
-
additional information
additional information
-
kinetic analysis of wild-type and mutant enzymes
-
additional information
additional information
stopped flow and steady-state kinetic analysis
-
additional information
additional information
-
stopped flow and steady-state kinetic analysis
-