1.1.1.86: ketol-acid reductoisomerase (NADP+)

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For detailed information about ketol-acid reductoisomerase (NADP+), go to the full flat file.

Word Map on EC 1.1.1.86

1.1.1.86

branched-chain

5-phosphate

karis

1-deoxy-d-xylulose

fosmidomycin

isoprenoids

methylerythritol

1-deoxy-d-xylulose-5-phosphate

deoxyxylulose

l-valine

isobutanol

2-c-methyl-d-erythritol

non-mevalonate

dihydroxyacid

2-keto

isoleucine-valine

ilvbnce

drug development

biofuel production

industry

Reaction

Synonyms

2-hydroxy-3-keto acid reductoisomerase, acetohydroxy acid isomeroreductase, acetohydroxy acid reductoisomerase, acetohydroxy-acid isomeroreductase , acetohydroxy-acid reductoisomerase , acetohydroxyacid isomeroreductase, acetolactate reductoisomerase, AHAIR, AHIR, alpha-keto-beta-hydroxylacil reductoisomerase, alpha-keto-beta-hydroxylacyl reductoisomerase, class II ketol-acid reductoisomerase, dehydrogenase, dihydroxyisovalerate (isomerizing), dihydroxyisovalerate dehydrogenase (isomerizing), EC 1.1.1.89, Icl1p, Ilv5p, ilvC, IlvC-PanE, IlvC1, IlvC2, isomerase, ketol acid reducto-, isomeroreductase, KARI, ketol-acid reductoisomerase, reductoisomerase

ECTree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.1.86 ketol-acid reductoisomerase (NADP+)

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Results	in table
1	Activating Compound
32999	AA Sequence
7	Application
1	CAS Registry Number
24	Cloned(Commentary)
58	Cofactor
24	Crystallization (Commentary)
37	General Information
2	General Stability
222	Inhibitors
137	kcat/KM [mM/s]
77	Ki Value [mM]
225	KM Value [mM]
5	Localization
62	Metals/Ions
28	Molecular Weight [Da]
72	Natural Substrates/ Products (Substrates)
125	Organism
12	Pathway
139	PDB ID
20	pH Optimum
7	pH Range
8	pH Stability
1	pI Value
1	Posttranslational Modification
52	Protein Variants
29	Purification (Commentary)
5	Reaction
4	Reaction Type
107	Reference
1	Renatured (Commentary)
10	Source Tissue
17	Specific Activity [micromol/min/mg]
3	Storage Stability
278	Substrates and Products (Substrate)
29	Subunits
123	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
2	Temperature Range [°C]
7	Temperature Stability [°C]
162	Turnover Number [1/s]

Purification

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Purification on EC 1.1.1.86 - ketol-acid reductoisomerase (NADP+)

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14 entries

mutant enzyme DELTA423-431/F431S

Spinacia oleracea

639185

Ni-NTA column chromatography

Meiothermus ruber

D3PT81

737785

Ni-NTA-agarose resin column chromatography and Superdex 200 gel filtration

Mycobacterium tuberculosis

P9WKJ7

738219

of wild-type and mutants

Escherichia coli

655530

partial

2 entries

recombinant enzyme from Escherichia coli

Spinacia oleracea

639179, 639181, 639182, 639185

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography

Oryza sativa

Q65XK0

670612

recombinant His6-tagged enzyme

Escherichia coli

670830

wild-type and mutant enzyme

Escherichia coli

639183