1.1.1.85: 3-isopropylmalate dehydrogenase This is an abbreviated version! For detailed information about 3-isopropylmalate dehydrogenase, go to the full flat file .
Reaction
(2R,3S)-3-isopropylmalate +
NAD+ =
(2S)-2-isopropyl-3-oxosuccinate +
NADH +
H+
Synonyms 2-hydroxy-4-methyl-3-carboxyvalerate:NAD+ oxidoreductase, 2R,3S-isopropylmalate:NAD+ oxidoreductase (decaboxylating), 3-IPM dehydrogenase, 3-IPM-DH, 3-isopropylmalate dehydrogenase, 3-isopropylmalateDH, APS IPMDH, beta-IPM dehydrogenase, beta-IPMDH, beta-isopropylmalate dehydrogenase, beta-isopropylmalic enzyme, Bs3-isopropylmalateDH, dehydrogenase, 3-isopropylmalate, IMDH, IPMDH, IPMDH1, IPMDH2, IPMDH3, isopropylmalate dehydrogenase, leuB, NAD-dependent isopropylmalate dehydrogenase, Saci_0600, SbIPMDH, SoIPMDH, threo-Ds-3-isopropylmalate dehydrogenase, Tt-beta-3-isopropylmalateDH, two-domain 3-isopropylmalate dehydrogenase, Ydr417cp
ECTree
Temperature Optimum
Temperature Optimum on EC 1.1.1.85 - 3-isopropylmalate dehydrogenase
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96
Tm-value of wild-type enzyme is 96°C. Tm-value of mutant enzyme K152R is 95.6°C. Tm-value of mutant enzyme (M91L/I95L/K152R/G154A/A259S/F261P/Y282L) is 96.4°C
98
Tm-value of mutant enzyme G154A is 97.9°C
additional information
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reaction temperature dependence, overview
50 - 55
-
-
60
-
-
70
-
-
75
-
-
95
-
95
Tm-value of mutant enzyme Y282L is 95.7°C
97
Tm-value of mutant enzyme A259S/F261P is 97.1°C
97
Tm-value of mutant enzyme K152R/G154A is 97.2°C
99
t1/2: 3.67 min (wild-type enzyme), 5.52 min (mutant enzyme M91L/I95L), 6.56 min (K152R/G154A mutant enzyme), 5.72 min (K152R mutant enzyme), 7.13 min (G154A mutant enzyme), 4.79 min (A259S/F261P mutant enzyme), 3.79 min (Y282L mutant enzyme), 6.5 min (M91L/I95L/K152R/G154A/A259S/F261P/Y282L mutant enzyme)
99
Tm-value of mutant enzyme M91L/I95L is 99.2°C