1.1.1.85: 3-isopropylmalate dehydrogenase This is an abbreviated version! For detailed information about 3-isopropylmalate dehydrogenase, go to the full flat file .
Reaction
(2R,3S)-3-isopropylmalate +
NAD+ =
(2S)-2-isopropyl-3-oxosuccinate +
NADH +
H+
Synonyms 2-hydroxy-4-methyl-3-carboxyvalerate:NAD+ oxidoreductase, 2R,3S-isopropylmalate:NAD+ oxidoreductase (decaboxylating), 3-IPM dehydrogenase, 3-IPM-DH, 3-isopropylmalate dehydrogenase, 3-isopropylmalateDH, APS IPMDH, beta-IPM dehydrogenase, beta-IPMDH, beta-isopropylmalate dehydrogenase, beta-isopropylmalic enzyme, Bs3-isopropylmalateDH, dehydrogenase, 3-isopropylmalate, IMDH, IPMDH, IPMDH1, IPMDH2, IPMDH3, isopropylmalate dehydrogenase, leuB, NAD-dependent isopropylmalate dehydrogenase, Saci_0600, SbIPMDH, SoIPMDH, threo-Ds-3-isopropylmalate dehydrogenase, Tt-beta-3-isopropylmalateDH, two-domain 3-isopropylmalate dehydrogenase, Ydr417cp
ECTree
Inhibitors
Inhibitors on EC 1.1.1.85 - 3-isopropylmalate dehydrogenase
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(2S,3S)-3-methylmercaptomalate
strong competitive inhibitor
1,2-Cyclohexanediamine-N,N,N',N'-tetraacetate
-
-
Fe2+
-
0.5 mM, 41% inhibition
NaCl
-
250 mM, 48% inhibition
NADH
-
NADH always produces competitive inhibition patterns with respect to NAD and noncompetitive inhibition patterns with respect to the substrates
O-isobutenyl oxalylhydroxamate
competitive inhibitor, binds the active site by adopting the same conformation of (2R,3S)-3-isopropylmalate, and to induce an unreported repositioning of the side chain of the amino acids that participate in the coordination of the ligand
-
O-isobutenyloxalylhydroxamate
O-isobutenyloxalylhydroxamate binds to the active site of enzyme in a mode similar to the substrate isopropylmalate
Ca2+
-
-
Ca2+
-
0.5 mM, 32% inhibition
Cu2+
-
-
Cu2+
-
0.5 mM, 77% inhibition
iodoacetamide
-
5 mM, 50% inhibition
iodoacetamide
-
50 mM, slight inhibition
iodoacetate
-
5 mM, 50% inhibition
iodoacetate
-
50 mM, slight inhibition
K+
-
above 1 M, slight inhibition
K+
-
250 mM KCl, 23% inhibition
NEM
-
5 mM, 50% inhibition
NEM
-
5 mM, 50% inhibition, not reversed by 2-mercaptoethanol
p-hydroxymercuribenzoate
-
pH 9.0, 2 mM, complete inhibition, reversed by 2-mercaptoethanol
p-hydroxymercuribenzoate
-
-
PCMB
-
5 mM, 50% inhibition
PCMB
-
0.2 mM, 90% inhibition