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0.2 - 5.8
(2S,3R)-tartrate
0.8
NAD+
-
cosubstrate malate
0.83
NADH
-
cosubstrate oxaloacetate
additional information
additional information
-
0.2
(2S,3R)-tartrate
pH 10.0, 50°C, with cofactor NAD+, recombinant enzyme
5.8
(2S,3R)-tartrate
pH 10.0, 50°C, with cofactor NADP+, recombinant enzyme
1.2
(2S,3S)-tartrate
pH 10.0, 50°C, with cofactor NAD+, recombinant enzyme
2 - 3
(2S,3S)-tartrate
pH 10.0, 50°C, with cofactor NADP+, recombinant enzyme
0.00012
(S)-malate
-
pH and temperature not specified in the publication
0.00012
(S)-malate
-
pH and temperature not specified in the publication
0.019
(S)-malate
pH 10.0, 50°C, with cofactor NADP+, recombinant enzyme
0.12
(S)-malate
pH 10.0, 50°C, with cofactor NAD+, recombinant enzyme
0.26
(S)-malate
-
pH and temperature not specified in the publication
0.4
(S)-malate
-
pH and temperature not specified in the publication
5
(S)-malate
Sorghum sp.
-
mutant enzyme D201A
10.2
(S)-malate
Sorghum sp.
-
mutant enzyme D201N
12
(S)-malate
Sorghum sp.
-
wild-type enzyme
29
(S)-malate
-
cosubstrate NAD+
32
(S)-malate
-
cosubstrate NADP+
0.000019
NADP+
-
pH and temperature not specified in the publication
0.000019
NADP+
-
pH and temperature not specified in the publication
0.019
NADP+
-
pH and temperature not specified in the publication
0.023
NADP+
-
pH and temperature not specified in the publication
0.073
NADP+
-
cosubstrate malate
0.13
NADP+
-
pH and temperature not specified in the publication
0.011
NADPH
-
-
0.0114
NADPH
Sorghum sp.
-
mutant enzyme D201N
0.02
NADPH
-
pH and temperature not specified in the publication
0.021
NADPH
-
mutant enzyme C23A/C28A and C23A/C28A/C376A, oxidized form
0.023
NADPH
-
mutant enzyme C23A/C28A/C206A/C364A, oxidized form
0.023
NADPH
Sorghum sp.
-
mutant enzyme D201A
0.024
NADPH
-
mutant enzyme C23A/C28A, reduced form
0.026
NADPH
-
mutant enzyme C23A/C28A/C364A and C23A/C28A/C364A/C376A, oxidized form
0.028
NADPH
-
mutant enzyme C23A/C28A/C206A/C376A, oxidized form
0.028
NADPH
Sorghum sp.
-
mutant enzyme C64S/C69S
0.028
NADPH
-
wild-type enzyme and mutant C28A, reduced enzyme form
0.029
NADPH
Sorghum sp.
-
mutant enzyme C207A/E387Q, oxidized form
0.029
NADPH
-
mutant enzyme lacking the N-terminal 45 residues, reduced form
0.03
NADPH
-
mutant enzyme C23A, reduced form
0.03
NADPH
-
recombinant enzyme cloned from expression vector pJ2PCC1
0.031
NADPH
Sorghum sp.
-
mutant enzyme C29S/C207A/E387Q, oxidized enzyme form
0.033
NADPH
-
mutant enzyme C23A/C28A/C206A, oxidized form and mutant DELTAN lacking the N-terminal 45 residues, reduced form
0.035
NADPH
-
enzyme from intermembrane space
0.035
NADPH
Sorghum sp.
-
mutant enzyme C29S/C207A/DELTAEV, oxidized form
0.036
NADPH
-
mutant enzyme C23A/C28A/C206A, reduced form
0.037
NADPH
Sorghum sp.
-
mutant C69S
0.038
NADPH
Sorghum sp.
-
mutant enzyme C64S
0.039
NADPH
Sorghum sp.
-
mutant enzyme DELTAN
0.039
NADPH
-
reduced native enzyme
0.042
NADPH
Sorghum sp.
-
wild-type enzyme
0.043
NADPH
-
recombinant enzyme cloned from expression vector pJCPCC2
0.044
NADPH
Sorghum sp.
-
mutant enzyme C29S/E387Q, reduced form
0.0446
NADPH
Sorghum sp.
-
wild-type enzyme
0.046
NADPH
Sorghum sp.
-
mutant enzyme C29S/E387Q, reduced form
0.046
NADPH
-
oxidized truncated enzyme
0.047
NADPH
Sorghum sp.
-
mutant enzyme C207A/DELTAEV, oxidized form
0.049
NADPH
Sorghum sp.
-
mutant enzyme C29S/DELTAEV, oxidized form
0.06
NADPH
Sorghum sp.
-
mutant enzyme C207A/DELTAEV, and mutant enzyme C29S/C207S/DELTAEV, reduced forms
0.063
NADPH
Sorghum sp.
-
mutant enzyme C29S/DELTAEV, reduced form
0.066
NADPH
-
enzyme from mitochondrial matrix
0.066
NADPH
Sorghum sp.
-
mutant enzyme C29S/C207A/E387Q, reduced form and mutant enzyme C29S/C207A/DELTAEV, reduced form
0.089
NADPH
-
reduced truncated enzyme
0.095
NADPH
-
reduced enzyme, + 200 mM guanidine
0.115
NADPH
Sorghum sp.
-
mutant enzyme C29S/C207A, reduced form
0.22
NADPH
-
oxidized enzyme, + 250 mM guanidine
0.472
NADPH
-
mutant enzyme lacking the N-terminal 45 residues, oxidized form
0.014
oxaloacetate
-
enzyme from intermembrane space
0.017
oxaloacetate
-
mutant enzyme C23A/C28A/C364A/C376A, oxidized form
0.019
oxaloacetate
-
recombinant enzyme cloned from expression vector pJ2PCC2
0.02
oxaloacetate
-
mutant enzyme C23A, reduced form
0.021
oxaloacetate
-
recombinant enzyme cloned from expression vector pJ2PCC1
0.022
oxaloacetate
Sorghum sp.
-
mutant enzyme DELTAN
0.022
oxaloacetate
-
mutant enzyme C23A/C28A, reduced form
0.024
oxaloacetate
-
mutant enzyme C23A/C28A/C206A/C376A, oxidized form
0.025
oxaloacetate
-
mutant enzyme C23A/C28A/C206A/C364A, oxidized form and mutant DELTAN, lacking the 45 N-terminal residues
0.027
oxaloacetate
-
wild-type enzyme, reduced form and mutant enzyme C23A/C28A/C364A, oxidized form
0.028
oxaloacetate
-
mutant enzyme C23A/C28A/C206A, reduced enzyme form and mutant enzyme C23A/C28A/C376A, oxidized form
0.03
oxaloacetate
-
pH and temperature not specified in the publication
0.037
oxaloacetate
Sorghum sp.
-
mutant enzyme C64S
0.037
oxaloacetate
-
enzyme from mitochondrial matrix
0.04
oxaloacetate
Sorghum sp.
-
wild-type enzyme
0.041
oxaloacetate
Sorghum sp.
-
wild-type enzyme
0.042
oxaloacetate
Sorghum sp.
-
mutant C69S
0.042
oxaloacetate
-
reduced truncated enzyme
0.044
oxaloacetate
Sorghum sp.
-
mutant enzyme C64S/C69S
0.048
oxaloacetate
-
reduced native enzyme
0.05
oxaloacetate
Sorghum sp.
-
mutant enzyme C39S/E387Q, reduced form
0.054
oxaloacetate
Sorghum sp.
-
mutant enzyme C207A/DELTAEV, reduced form
0.056
oxaloacetate
-
cosubstrate NADPH
0.056
oxaloacetate
Sorghum sp.
-
mutant enzyme C29S/E387Q, reduced form
0.061
oxaloacetate
-
cosubstrate NADH
0.07
oxaloacetate
Sorghum sp.
-
mutant enzyme C29S/DELTAEV, reduced form
0.077
oxaloacetate
-
reduced enzyme, + 200 mM guanidine
0.086
oxaloacetate
Sorghum sp.
-
mutant enzyme C29S/C207A/E387Q, reduced form
0.094
oxaloacetate
Sorghum sp.
-
mutant enzyme C29S/C207A, reduced form
0.105
oxaloacetate
-
mutant enzyme C23A/C28A/C206A, oxidized form
0.32
oxaloacetate
-
oxidized enzyme, + 250 mM guanidine
0.447
oxaloacetate
Sorghum sp.
-
mutant enzyme C207A/DELTAEV, oxidized form
0.578
oxaloacetate
Sorghum sp.
-
mutant enzyme C29S/DELTAEV, oxidized form
0.583
oxaloacetate
Sorghum sp.
-
mutant enzyme C29S/E387Q, oxidized form
0.894
oxaloacetate
Sorghum sp.
-
mutant enzyme C29S/C207A/E387Q, oxidized form
0.931
oxaloacetate
Sorghum sp.
-
mutant enzyme C29S/C207A/DELTAEV, oxidized form
1.2
oxaloacetate
-
oxidized truncated enzyme
1.8
oxaloacetate
Sorghum sp.
-
mutant enzyme D201N
3
oxaloacetate
Sorghum sp.
-
mutant enzyme D201A
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-