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0.3
alpha-glycerol phosphate
-
-
0.11 - 3.39
alpha-glycerophosphate
0.012 - 25
dihydroxyacetone phosphate
0.19 - 1.2
DL-glycerol-3-phosphate
0.59 - 2
glycerol 3-phosphate
1.6
glycerol phosphate
-
-
0.468 - 34
glycerol-3-phosphate
0.052 - 137
glycerone phosphate
2.3 - 3.9
L-glycerol-3-phosphate
0.41 - 469
sn-glycerol 3-phosphate
0.143
sn-glycerol-3-phosphate
-
measured at pH 9.0
additional information
alpha-glycerophosphate
0.11
alpha-glycerophosphate
-
-
0.33
alpha-glycerophosphate
-
testes
3.39
alpha-glycerophosphate
-
liver
0.012
dihydroxyacetone phosphate
-
-
0.026
dihydroxyacetone phosphate
-
measured at pH 7.5
0.052
dihydroxyacetone phosphate
wild type enzyme, at pH 7.5 and 25°C
0.075
dihydroxyacetone phosphate
-
-
0.1
dihydroxyacetone phosphate
-
-
0.12
dihydroxyacetone phosphate
-
-
0.13
dihydroxyacetone phosphate
-
standard deviation 0.03 mM
0.15
dihydroxyacetone phosphate
-
muscle
0.17
dihydroxyacetone phosphate
-
-
0.2
dihydroxyacetone phosphate
-
I6.5
0.2144
dihydroxyacetone phosphate
-
alpha-GPDHs, standard deviation 0.0215 mM
0.23
dihydroxyacetone phosphate
-
-
0.23
dihydroxyacetone phosphate
-
in MOPS buffer, 0.33 mM in Tris-histidine
0.25
dihydroxyacetone phosphate
-
adipose tissue
0.3
dihydroxyacetone phosphate
-
0.30-0.35 mM
0.33
dihydroxyacetone phosphate
-
-
0.46
dihydroxyacetone phosphate
-
-
0.52
dihydroxyacetone phosphate
-
GPDH-1
0.54
dihydroxyacetone phosphate
-
-
0.54
dihydroxyacetone phosphate
at pH 7.0 and 25°C
0.61
dihydroxyacetone phosphate
at pH 7.0 and 25°C
5.7
dihydroxyacetone phosphate
mutant enzyme R269A, at pH 7.5 and 25°C
15
dihydroxyacetone phosphate
mutant enzyme R269A/N270A, at pH 7.5 and 25°C
25
dihydroxyacetone phosphate
mutant enzyme N270A, at pH 7.5 and 25°C
0.19
DL-glycerol-3-phosphate
-
muscle
0.3
DL-glycerol-3-phosphate
-
I6.5
0.4
DL-glycerol-3-phosphate
-
adipose tissue
1.2
DL-glycerol-3-phosphate
-
standard deviation 0.5 mM
0.59
glycerol 3-phosphate
-
GPDH-1
0.909
glycerol 3-phosphate
-
-
1.89
glycerol 3-phosphate
-
alpha-GPDHs, standard deviation 0.25 mM
2
glycerol 3-phosphate
-
-
0.468
glycerol-3-phosphate
-
pH 9.0, 25°C
1.7
glycerol-3-phosphate
-
-
2.25
glycerol-3-phosphate
-
-
34
glycerol-3-phosphate
-
isoform Gut2, pH 6.8
0.052
glycerone phosphate
wild-type enzyme, pH 7.5, 25°C
0.137
glycerone phosphate
-
pH 7.6, 25°C
0.154
glycerone phosphate
-
pH 7.5, 5°C, muscle isozyme
0.259
glycerone phosphate
-
pH 7.5, 5°C, liver isozyme
0.26
glycerone phosphate
-
pH 7.5, 25°C
0.279
glycerone phosphate
-
pH 7.5, 22°C, muscle isozyme
0.352
glycerone phosphate
-
pH 7.5, 22°C, liver isozyme
1.5
glycerone phosphate
mutant R269A/N270A, pH 7.5, 25°C
2.5
glycerone phosphate
mutant N270A, pH 7.5, 25°C
5.7
glycerone phosphate
mutant R269A, pH 7.5, 25°C
137
glycerone phosphate
-
pH 9.0, 25°C
2.3
L-glycerol-3-phosphate
-
pH 10.0, 25°C
3.9
L-glycerol-3-phosphate
-
pH 7.5, 25°C
0.0044
NAD+
-
adipose tissue
0.036
NAD+
-
measured at pH 9.0
0.045
NAD+
-
pH 8.5, 22°C, liver isozyme
0.09
NAD+
-
pH 8.5, 5°C, liver isozyme
0.09
NAD+
-
pH 8.0, 5°C, hibernation G3PDH enzyme
0.1003
NAD+
-
alpha-GPDHs, standard deviation 0.0041 mM
0.12
NAD+
-
pH 8.0, 5°C, euthermic G3PDH enzyme
0.143
NAD+
-
pH 8.5, 22°C, muscle isozyme
0.155
NAD+
-
pH 8.5, 5°C, muscle isozyme
0.2
NAD+
-
enzyme from euthermic animal, at pH 7.5 and 22°C
0.2
NAD+
-
enzyme from hibernating animal, at pH 7.5 and 22°C
0.2
NAD+
-
pH 7.5, 22°C, euthermic G3PDH enzyme and hibernation G3PDH enzyme
0.3
NAD+
-
standard deviation 0.06 mM
0.316
NAD+
-
pH 9.0, 25°C
0.32
NAD+
-
enzyme from hibernating animal, at pH 8.0 and 37°C
0.32
NAD+
-
pH 8.0, 37°C, hibernation G3PDH enzyme
0.39
NAD+
-
enzyme from euthermic animal, at pH 8.0 and 37°C
0.39
NAD+
-
pH 8.0, 37°C, euthermic G3PDH enzyme
0.42
NAD+
-
enzyme from hibernating animal, at pH 8.0 and 22°C
0.42
NAD+
-
pH 8.0, 22°C, hibernation G3PDH enzyme
0.5
NAD+
-
enzyme from euthermic animal, at pH 7.5 and 37°C
0.5
NAD+
-
pH 7.5, 37°C, euthermic G3PDH enzyme
0.52
NAD+
-
enzyme from euthermic animal, at pH 8.0 and 22°C
0.52
NAD+
-
pH 8.0, 22°C, euthermic G3PDH enzyme
0.69
NAD+
-
enzyme from hibernating animal, at pH 7.5 and 37°C
0.69
NAD+
-
pH 7.5, 37°C, hibernation G3PDH enzyme
0.001
NADH
-
pH 7.5, 22°C, liver isozyme
0.001
NADH
-
pH 7.5, 5°C, liver isozyme
0.001
NADH
-
pH 7.5, 5°C, muscle isozyme
0.003
NADH
-
pH 7.5, 22°C, muscle isozyme
0.0043
NADH
-
adipose tissue
0.005
NADH
-
pH 7.5, 25°C
0.005
NADH
-
measured at pH 7.5
0.00526
NADH
-
alpha-GPDHs
0.0066
NADH
-
standard deviation 0.003 mM
0.01
NADH
-
less than 0.01 mM
0.01
NADH
-
less than 0.01 mM
0.032
NADH
-
pH 7.6, 25°C
0.051
NADH
at pH 7.0 and 25°C
0.0589
NADH
recombinant isozyme GDH2
0.0592
NADH
isozyme GPDH2
0.0905
NADH
isozyme GPDH1
0.0905
NADH
recombinant isozyme GDH1
0.11
NADH
at pH 7.0 and 25°C
0.0589
NADPH
isozyme GPDH1
0.0592
NADPH
recombinant isozyme GDH1
0.0726
NADPH
isozyme GPDH2
0.0726
NADPH
recombinant isozyme GDH2
0.41
sn-glycerol 3-phosphate
-
enzyme from hibernating animal, at pH 7.5 and 22°C
0.41
sn-glycerol 3-phosphate
-
pH 7.5, 22°C, hibernation G3PDH enzyme
0.48
sn-glycerol 3-phosphate
-
enzyme from hibernating animal, at pH 8.0 and 5°C
0.48
sn-glycerol 3-phosphate
-
pH 8.0, 5°C, hibernation G3PDH enzyme
0.57
sn-glycerol 3-phosphate
-
enzyme from euthermic animal, at pH 8.0 and 22°C
0.57
sn-glycerol 3-phosphate
-
pH 8.0, 22°C, euthermic G3PDH enzyme
0.61
sn-glycerol 3-phosphate
-
enzyme from euthermic animal, at pH 7.5 and 22°C
0.61
sn-glycerol 3-phosphate
-
pH 7.5, 22°C, euthermic G3PDH enzyme
0.63
sn-glycerol 3-phosphate
-
enzyme from euthermic animal, at pH 7.5 and 37°C
0.63
sn-glycerol 3-phosphate
-
pH 7.5, 37°C, euthermic G3PDH enzyme
0.74
sn-glycerol 3-phosphate
-
-
0.83
sn-glycerol 3-phosphate
-
enzyme from euthermic animal, at pH 8.0 and 5°C
0.83
sn-glycerol 3-phosphate
-
pH 8.0, 5°C, euthermic G3PDH enzyme
0.88
sn-glycerol 3-phosphate
-
enzyme from euthermic animal, at pH 8.0 and 37°C
0.88
sn-glycerol 3-phosphate
-
pH 8.0, 37°C, euthermic G3PDH enzyme
1.17
sn-glycerol 3-phosphate
-
enzyme from hibernating animal, at pH 7.5 and 37°C
1.17
sn-glycerol 3-phosphate
-
pH 7.5, 37°C, hibernation G3PDH enzyme
1.51
sn-glycerol 3-phosphate
-
enzyme from euthermic animal, at pH 7.5 and 5°C
1.51
sn-glycerol 3-phosphate
-
pH 7.5, 5°C, euthermic G3PDH enzyme
1.56
sn-glycerol 3-phosphate
-
enzyme from hibernating animal, at pH 8.0 and 37°C
1.56
sn-glycerol 3-phosphate
-
pH 8.0, 37°C, hibernation G3PDH enzyme
1.61
sn-glycerol 3-phosphate
-
enzyme from hibernating animal, at pH 8.0 and 22°C
1.61
sn-glycerol 3-phosphate
-
pH 8.0, 22°C, hibernation G3PDH enzyme
469
sn-glycerol 3-phosphate
-
pH 7.6, 25°C
additional information
alpha-glycerophosphate
-
Km values from five different isoelectric focused components
additional information
additional information
-
various Km-values of 4 isozymes of heart, muscle, liver and mammary gland
-
additional information
additional information
-
Km values of isozyme I5.9
-
additional information
additional information
-
Km values of two further allelic isozymes, alpha-GPDHf and alpha-GPDHm
-
additional information
additional information
-
Km values of isozyme GPDH-3
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
kinetic analysis, overview. Relative to euthermic liver G3PDH, hibernator liver G3PDH has a decreased affinity for its substrate, glycerol-3-phosphate, at 37°C and 22°C, while at 5°C, there is a significant increase in the affinity for G3P in the hibernating form of the enzyme, relative to the euthermic form. A change in pH from pH 8.0 to pH 7.5 leads to increased G3PDH affinity for NAD+. With a change in pH from pH 8.0 to pH 7.5 at 22°C in the euthermic and hibernator conditions, the Km for NAD+ decreases by 62% and 53%, respectively
-