expression in Saccharomyces cerevisiae. Although the gene is functional in Saccharomyces cerevisiae, its heterologous expression is not efficient, suggesting that the regulatory mechanism may not be shared by Debaryomyces hansenii and Saccharomyces cerevisiae
gene Cagpd1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, the intronless genes Cagpd1 and Cagpd2, encoding the two isozymes of the organism, are both predicted to encode a 378 amino acid polypeptide, and the deduced amino acid sequences mutually show 76% identity. Genes Cagpd1 and Cagpd2 are located tandemly in a locus of genomic DNA within a 262 bp interval. Recombinant expression in the Sacchhyromyces cerevisiae strain BY4741 gpd1DELTAgpd2DELTA double mutant
gene Cagpd2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, the intronless genes Cagpd1 and Cagpd2, encoding the two isozymes of the organism, are both predicted to encode a 378 amino acid polypeptide, and the deduced amino acid sequences mutually show 76% identity. Genes Cagpd1 and Cagpd2 are located tandemly in a locus of genomic DNA within a 262 bp interval. Recombinant expression in the Sacchhyromyces cerevisiae strain BY4741 gpd1DELTAgpd2DELTA double mutant
gene GPDH1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene SFD1, overexpression of the enzyme under the control of the maize ubiquitin promoter in Oryza sativa Japonica rice cultivar TP309 chloroplasts by transformation of embryogenic calli using Agrobacterium tumefaciens strain LBA4404, SFD1-GFP localization in rice leaf sheath cells