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1.1.1.71: alcohol dehydrogenase [NAD(P)+]

This is an abbreviated version!
For detailed information about alcohol dehydrogenase [NAD(P)+], go to the full flat file.

Word Map on EC 1.1.1.71

Reaction

a primary alcohol
+
NAD(P)+
=
an aldehyde
+
NAD(P)H
+
H+

Synonyms

ADH, ADH12, ADH2, Adh319, AdhA, AdhE, alcohol dehydrogenase, aldehyde reductase (NADPH/NADH), DHRS3, HvADH2, HVO_B0071, NAD(P)+-dependent alcohol dehydrogenase, NAD(P)H-dependent ADH, NAD(P)H-dependent aldehyde reductase, NADPH-dependent ADHA, NADPH-dependent alcohol dehydrogenase, PH0743, PhADH, RADH, retinal reductase, retinal short-chain dehydrogenase/reductase member 3, retinaldehyde reductase, Retinol dehydrogenase, retinol-active alcohol dehydrogenase, retSDR1, TeSADH, TsAdh319, Tsib_0319, VNG_2617G, YqhD

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.71 alcohol dehydrogenase [NAD(P)+]

Temperature Stability

Temperature Stability on EC 1.1.1.71 - alcohol dehydrogenase [NAD(P)+]

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
102
Tm-value in presence of 1 M NaCl
104
Tm-value in absence of NaCl
30
-
no loss of activity after 15 min, complete loss of activity at 60°C
4 - 60
the enzyme is thermally stable when incubated for 30 min between 4 and 40°C. Incubation for 30 min at temperatures between 10 and 30 °C slightly increases the catalytic activity. Recombinant enzyme is completely inactivated at 50 and 60°C
41
-
melting temperature T1/2 of wild-type enzyme ADHA
48
-
melting temperature T1/2 of mutant ADHA-0278
53
-
melting temperature T1/2 of mutant ADHA-0279
65
-
melting temperature T1/2 of mutant ADHA-0391
66
-
melting temperature T1/2 of mutant ADHA-0384
67
-
melting temperature T1/2 of mutant ADHA-0398 and of mutant ADHA-0381
70 - 95
the enzyme activity is slightly decreased by 1 h incubation at 70°C (2.6%), 80°C (4.7%), 90°C (7.9%), and 95°C (18.8%), 50% loss of activity after approximately 6 h at 95°C
8 - 25
-
the enzyme has a half-life of 80 h at 25°C and stability optimum between 8 and 15°C in the presence of 0.8 mM CaCl2 (half-life of 130 h)
95
-
the highly thermostable enzyme has a half-life of 4.5 h at 95°C and 16.2 h at 85°C
additional information
thermal denaturation is fully irreversible and follows first-order kinetics