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1.1.1.67: mannitol 2-dehydrogenase

This is an abbreviated version!
For detailed information about mannitol 2-dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.67

Reaction

D-mannitol
+
NAD+
=
D-fructose
+
NADH
+
H+

Synonyms

alcohol dehydrogenase, zinc-containing, D-mannitol dehydrogenase, M2DH, mannitol 2-dehydrogenase, mannitol dehydrogenase, mannitol-2-dehydrogenase, MDH, mt-dh, MtDH, MtlD, NAD+-dependent mannitol dehydrogenase, NADH-dependent mannitol dehydrogenase, pfMDH, polyol dehydrogenase, PsM2DH, TM0298, TM_0298

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.67 mannitol 2-dehydrogenase

Purification

Purification on EC 1.1.1.67 - mannitol 2-dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by heat treatment at 85°C and on Ni-NTA column
by Ni2+-NTA affinity chromatography
-
Co2+ chelating Sepharose column chromatography and Superdex 200 gel filtration
HisBind affinity chromatography
-
HisPur cobalt resin column chromatography
histidine-tagged recombinant protein, expressed in Escherichia coli
-
HisTrap column chromatography
-
mutants purified to apparent homogeneity
Ni-NTA agarose column chromatography
recombinant enzyme from Escherichia coli strain JM109
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant protein