1.1.1.6: glycerol dehydrogenase
This is an abbreviated version!
For detailed information about glycerol dehydrogenase, go to the full flat file.
Reaction
Synonyms
B4100_2156, CglD, dehydrogenase, glycerol, GDH, GDH2, GLD, Gld3, GldA, GLDH
ECTree
Advanced search results
Substrates Products
Substrates Products on EC 1.1.1.6 - glycerol dehydrogenase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
REACTION DIAGRAM
1,2,3-butanetriol + NAD+
1,3-dihydroxybutane-2-one + NADH
-
-
-
?
1,2-butanediol + NAD+
1-hydroxy-2-butanone + NADH + H+
-
-
-
?
1,2-ethanediol + NAD+
hydroxyacetaldehyde + NADH + H+
-
-
-
?
1,3-butanediol + NAD+
?
-
relative activity is 6.5% compared to oxidation of glycerol
-
-
?
3-(2,2,2-trifluoroethoxy)propane-1,2-diol + NAD+
1-hydroxy-3-(2,2,2-trifluoroethoxy)propan-2-one + NADH + H+
-
-
-
?
3-(2,2,2-trifluoroethoxy)propane-1,2-diol + NAD+
? + NADH + H+
-
-
-
?
3-butoxypropane-1,2-diol + NAD+
1-hydroxy-3-butoxypropan-2-one + NADH + H+
-
-
-
?
3-chloro-1,2-propanediol + NAD+
?
-
130% of the activity with glycerol
-
-
?
3-ethoxypropane-1,2-diol + NAD+
1-hydroxy-3-ethoxypropan-2-one + NADH + H+
-
-
-
?
3-mercapto-1,2-propanediol + NAD+
?
-
155% of the activity with glycerol
-
-
?
3-methoxypropane-1,2-diol + NAD+
1-hydroxy-3-methoxypropan-2-one + NADH + H+
-
-
-
?
3-phenoxypropane-1,2-diol + NAD+
1-hydroxy-3-phenoxypropan-2-one + NADH + H+
-
-
-
?
3-propoxypropane-1,2-diol + NAD+
1-hydroxy-3-propoxypropan-2-one + NADH + H+
-
-
-
?
3-[(propan-2-yl)oxy]propane-1,2-diol + NAD+
1-hydroxy-3-[(propan-2-yl)oxy]propan-2-one + NADH + H+
-
-
-
?
3-[(propan-2-yl)oxy]propane-1,2-diol + NAD+
? + NADH + H+
-
-
-
?
beta-glycerophosphate + NAD+
?
-
relative activity is 2% compared to oxidation of glycerol
-
-
?
diglycerol + NAD+
?
-
relative activity is 21% compared to oxidation of glycerol
-
-
?
ethanol + NAD+
acetaldehyde + NADH
-
relative activity is 1% compared to oxidation of glycerol
-
-
?
hydroxy-2-propanone + NADH
propylene glycol + NAD+
-
relative activity is 27% compared to oxidation of glycerol
-
-
?
i-inositol + NAD+
?
-
relative activity is 18% compared to oxidation of glycerol
-
-
?
isopropanol + NAD+
acetone + NADH
-
relative activity is 17% compared to oxidation of glycerol
-
-
?
sorbitol + NAD+
?
-
relative activity is 3% compared to oxidation of glycerol
-
-
?
(3R)-acetoin + NADH
-
-
more than 99% enantiomeric excess of R-product
-
r
(2R,3R)-2,3-butanediol + NAD+
(3R)-acetoin + NADH
-
-
more than 99% enantiomeric excess of R-product
-
r
? + NADH
-
better substrate than glycerol
-
-
?
(2R,3R)-2,3-butanediol + NAD+
? + NADH
-
better substrate than glycerol
-
-
?
(R)-1,2-propanediol + NAD+
? + NADH
-
better substrate than glycerol
-
-
?
hydroxyacetone + NADH
the enzyme prefers the R-enantiomer
-
-
r
(R)-1,2-propanediol + NAD+
hydroxyacetone + NADH
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
the enzyme prefers the R-enantiomer
-
-
r
1,2-propanediol + NAD+
hydroxyacetone + NADH
-
105% of the activity with glycerol
-
-
?
1,2-propanediol + NAD+
hydroxyacetone + NADH
-
relative activity is 116% compared to oxidation of glycerol
-
-
?
1,2-propanediol + NAD+
hydroxyacetone + NADH
-
relative activity is 116% compared to oxidation of glycerol
-
-
?
1,2-propanediol + NAD+
hydroxyacetone + NADH
-
as active as glycerol
-
-
?
1,2-propanediol + NAD+
hydroxyacetone + NADH
-
1,2-propanediol represses the global activator HilA that induces an invasive phenotype and repression of HilA could be weakened by glycerol dehydrogenase activity
-
-
?
1,3-butanediol + NAD+
4-hydroxy-2-butanone + NADH + H+
-
-
-
-
?
1,3-butanediol + NAD+
4-hydroxy-2-butanone + NADH + H+
-
-
-
-
?
1,3-butanediol + NAD+
4-hydroxy-2-butanone + NADH + H+
-
-
-
?
1,3-dichloro-2-propanone + NADH
-
-
-
-
?
1,3-dichloro-2-propanol + NAD+
1,3-dichloro-2-propanone + NADH
-
-
-
-
?
?
-
relative rate of oxidation is 18% compared to oxidation of glycerol
-
-
?
1,3-propanediol + NAD+
?
-
relative rate of oxidation is 37% compared to oxidation of glycerol
-
-
?
?
-
relative activity is 0.3% compared to oxidation of glycerol
-
-
?
1,4-butanediol + NAD+
?
-
relative activity 17% compared to oxidation of glycerol
-
-
?
2,3-butanediol + NAD+
3-hydroxybutane-2-one + NADH
-
as active as glycerol
-
-
?
?
-
oxidation at 16% compared to oxidation of glycerol
-
-
?
DL-alpha-glycerophosphate + NAD+
?
-
relative activity 11%compared to oxidation of glycerol
-
-
?
DL-glyceraldehyde + NAD+
3-hydroxypyruvaldehyde + NADH
-
relative rate of reduction is 14%compared to reduction of dihydroxyacetone
-
-
?
DL-glyceraldehyde + NAD+
3-hydroxypyruvaldehyde + NADH
-
-
-
-
?
DL-glyceraldehyde + NAD+
3-hydroxypyruvaldehyde + NADH
-
-
-
?
glycolaldehyde + NADH
-
-
reduction at concentration of 50 mM
r
ethanediol + NAD+
glycolaldehyde + NADH
-
-
reduction at concentration of 50 mM
r
ethylene glycol + NAD+
?
-
relative activity is 20% compared to oxidation of glycerol
-
-
?
glycerone + N6-carboxymethyl-NADH + H+
-
-
-
r
glycerol + N6-carboxymethyl-NAD+
glycerone + N6-carboxymethyl-NADH + H+
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
-
-
r
glycerone + N6-carboxymethyl-NADH + H+
-
-
-
r
glycerol + N6-CM-NAD+
glycerone + N6-carboxymethyl-NADH + H+
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
-
-
r
glycerol + NAD+
D-glyceraldehyde + NADH + H+
-
no activity with NADP+. The enzyme shows much higher activity towards glycerol as compared to short chain primary and secondary alcohols. The thermostable enzyme is highly stereospecific in oxidation of glycerol and converts glycerol into D-glyceraldehyde
-
-
r
glycerol + NAD+
D-glyceraldehyde + NADH + H+
Thermus thermophilus MTCC 1494
-
no activity with NADP+. The enzyme shows much higher activity towards glycerol as compared to short chain primary and secondary alcohols. The thermostable enzyme is highly stereospecific in oxidation of glycerol and converts glycerol into D-glyceraldehyde
-
-
r
glycerol + NAD+
dihydroxyacetone + NADH + H+
-
-
-
-
?
glycerol + NAD+
dihydroxyacetone + NADH + H+
-
Glycerol dehydrogenase was immobilised in a polycarbamoyl sulfonate-hydrogel to be used as a sensor for glycerol. Glycerol oxidation leads to the reduction of NAD+ to NADH and electrons are transferred to ferricyanide on an electrode surface
-
-
?
glycerol + NAD+
dihydroxyacetone + NADH + H+
-
the reaction is performed in reverse micelles harboring glycerol and NAD+ in a solution of isooctane containing 250 mM diocytlsulfosuccinate
-
-
?
glycerol + NAD+
dihydroxyacetone + NADH + H+
-
the reaction is performed in reverse micelles harboring glycerol and NAD+ in a solution of isooctane containing 250 mM diocytlsulfosuccinate
-
-
?
glycerol + NAD+
dihydroxyacetone + NADH + H+
-
-
-
-
?
glycerol + NAD+
dihydroxyacetone + NADH + H+
-
-
-
?
glycerol + NAD+
dihydroxyacetone + NADH + H+
the enzyme exhibits an exclusive preference for NAD+ over NADP+
-
-
?
glycerol + NAD+
dihydroxyacetone + NADH + H+
Klebsiella pneumoniae subsp. pneumoniae MGH78578 ATCC 700721
the enzyme exhibits an exclusive preference for NAD+ over NADP+
-
-
?
glycerone + NADH + H+
activity of the enzyme is confirmed by proteome analysis and enzyme assays with cell extract glycerol-grown cells
-
-
?
glycerol + NAD+
glycerone + NADH + H+
-
-
-
r
glycerol + NAD+
glycerone + NADH + H+
no activity detected with NADP+
-
-
?
glycerol + NAD+
glycerone + NADH + H+
no activity detected with NADP+
-
-
?
glycerol + NAD+
glycerone + NADH + H+
-
glycerone reduction is the dominant reaction
i.e. 1,3-dihydroxypropranone
-
r
glycerol + NAD+
glycerone + NADH + H+
-
-
-
-
r
glycerol + NAD+
glycerone + NADH + H+
-
glycerone reduction is the dominant reaction
i.e. 1,3-dihydroxypropranone
-
r
dihydroxyacetone + NADPH + H+
the enzyme exhibits an exclusive preference for NAD+ over NADP+
-
-
?
glycerol + NADP+
dihydroxyacetone + NADPH + H+
Klebsiella pneumoniae subsp. pneumoniae MGH78578 ATCC 700721
the enzyme exhibits an exclusive preference for NAD+ over NADP+
-
-
?
glycerol + NADP+
glyceraldehyde + NADPH + H+
-
activity observed with pentan-1-ol, 3-methyl-butan-1-ol, 1-decanol, low activity as ethanol dehydrogenase with NAD+ or NADPH+ as cofactor
-
-
?
glycerol + NADP+
glyceraldehyde + NADPH + H+
-
activity observed with pentan-1-ol, 3-methyl-butan-1-ol, 1-decanol, low activity as ethanol dehydrogenase with NAD+ or NADPH+ as cofactor
-
-
?
?
-
relative rate of oxidation is 71% compared to oxidation of glycerol
-
-
?
glycerol-alpha-monochlorohydrin + NAD+
?
-
relative rate of oxidation is 26% compared to oxidation of glycerol
-
-
?
(3S)-acetoin + NADH
-
-
more than 99% enantiomeric excess of S-product
-
?
meso-2,3-butanediol + NAD+
(3S)-acetoin + NADH
-
-
more than 99% enantiomeric excess of S-product
-
?
lactaldehyde + NAD+
-
relative rate of reduction is 56% compared to reduction of dihydroxyacetone
-
-
?
3-hydroxybutane-2-one + NADH + H+
-
-
-
?
racemic 2,3-butanediol + NAD+
3-hydroxybutane-2-one + NADH + H+
Klebsiella pneumoniae subsp. pneumoniae MGH78578 ATCC 700721
-
-
-
?
?
-
-
ability of the enzyme to use glycerol from biodiesel waste as substrate, overview
-
-
?
additional information
?
-
-
efficiency of a cofactor regeneration enzyme co-expressed with a glycerol dehydrogenase for the production of 1,3-dihydroxyacetone. In vitro biotransformation of glycerol is achieved with the cell-free extracts containing recombinant glycerol dehydrogenase from Escherichia coli, lactate dehydrogenase form Bacillus subtilis, or NADH oxidase LpNox1 from Lactobacillus pentosus, giving1,3-dihydroxyacetone, overview
-
-
?
additional information
?
-
enzyme is highly enantioselective towards S-isomers of substituted glycerol derivatives
-
-
-
additional information
?
-
-
enzyme is highly enantioselective towards S-isomers of substituted glycerol derivatives
-
-
-
additional information
?
-
the enzyme is also active with substrates 4-chloroacetoacetate, 3-chloroacetylpyridine, 4-chloroacetophenone, and acetophenone, substrate specificities of enzyme with bound Zn2+, Mn2+, or Mg2+, overview
-
-
?
additional information
?
-
the enzyme is also active with substrates 4-chloroacetoacetate, 3-chloroacetylpyridine, 4-chloroacetophenone, and acetophenone, substrate specificities of enzyme with bound Zn2+, Mn2+, or Mg2+, overview
-
-
?
additional information
?
-
-
high specificity of enzyme for secondary alcohols in R-configuration
-
-
?
additional information
?
-
-
no substrate: 1,3-propanediol, ethanol, 1-propanol, 2-propanol, propionic acid, 1,4-butanediol, sorbitol, L-iditol. Stereospecificity for R-form
-
-
?
additional information
?
-
-
high specificity of enzyme for secondary alcohols in R-configuration
-
-
?
additional information
?
-
-
no substrate: 1,3-propanediol, ethanol, 1-propanol, 2-propanol, propionic acid, 1,4-butanediol, sorbitol, L-iditol. Stereospecificity for R-form
-
-
?
additional information
?
-
-
enzyme TtGlyDH preferentially catalyzes 1,3-dihydroxypropranone reduction rather than alcohol compound oxidation. Glycerol oxidization activity is faintly detected in the presence of a high concentration of glycerol (137 mM). No activity is detected with primary alcohols or diols. The highest glycerol oxidation activity is observed at the optimal growth temperature of 60°C in Tris-HCl buffer (50 mM, pH 8.0). Maximum DHA reduction activity is also observed at 60°C, and TtGlyDH exhibits the highest activity in an acetate buffer, compared with 91% maximum activity in an imidazole buffer at the same pH of 6.0
-
-
?
additional information
?
-
-
enzyme TtGlyDH preferentially catalyzes 1,3-dihydroxypropranone reduction rather than alcohol compound oxidation. Glycerol oxidization activity is faintly detected in the presence of a high concentration of glycerol (137 mM). No activity is detected with primary alcohols or diols. The highest glycerol oxidation activity is observed at the optimal growth temperature of 60°C in Tris-HCl buffer (50 mM, pH 8.0). Maximum DHA reduction activity is also observed at 60°C, and TtGlyDH exhibits the highest activity in an acetate buffer, compared with 91% maximum activity in an imidazole buffer at the same pH of 6.0
-
-
?