1.1.1.47: glucose 1-dehydrogenase [NAD(P)+]
This is an abbreviated version!
For detailed information about glucose 1-dehydrogenase [NAD(P)+], go to the full flat file.
Word Map on EC 1.1.1.47
Reaction
Synonyms
alkali-resistant glucose 1-dehydrogenase, beta-D-glucose:NAD(P)+ 1-oxido-reductase, beta-D-glucose:NAD(P)+ 1-oxidoreductase, BmGlcDH-III, BmGlcDH-IV, bzgdh, D-glucose dehydrogenase (NAD(P)), EC 1.1.5.2, GDH, gdh-2, GDHB, general stress protein 74, GlcDH, GlcDH-I, GlcDH-II, GlcDH-IWG3, glucose 1-dehydrogenase, glucose 1-dehydrogenase 3, glucose 1-dehydrogenase B, glucose 1-dehydrogenase III, glucose 1-dehydrogenase IV, glucose dehydrogenase, glucose1-dehydrogenase, GluDH, GSP74, hexose phosphate dehydrogenase, hexose-6-phosphate dehydrogenase, LsGDH, More, NAD(P)-dependent glucose 1-dehydrogenase, NAD(P)-dependent glucose dehydrogenase, NAD(P)-dependent glucose-1-dehydrogenase, NAD-GDH, nicotinamide adenine dinucleotide (NAD)-linked glucose dehydrogenase, SsGDH, SSO3204
ECTree
1.1.1.47 glucose 1-dehydrogenase [NAD(P)+]Advanced search results
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results (Temperature Stability
Temperature Stability on EC 1.1.1.47 - glucose 1-dehydrogenase [NAD(P)+]
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TEMPERATURE STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
25 - 35
stable for 1 week at 30°C (pH 6.5), completely inactivated in 30 min at 55°C, limited protection by glycerol, 84% activity after 30 min at 65°C in the presence of 2 M NaCl,
25 - 80
-
the enzyme shows considerable thermal stability after incubation for 30 min at temperature ranging from 25 to 80°C and maintains 20% of its maximum activity at 70°C
30
-
20 min, isoenzyme GlcDH-III, without NaCl, about 55% loss of activity
30 - 65
-
after 30 min at 30-65°C, the enzyme maintains high activity of more than 85%
40
45
50
55
60
65
70
-
20 min, presence of 2 mM NaCl, complete inactivation of isoenzyme GlcDH-III and GlcDH-IV, isoenzyme GlcDH-IWG3 loses 30% of initial activity
additional information
45
-
20 min, isoenzyme GlcDH-IV, without NaCl, about 35% loss of activity, isoenzyme IWG3, without NaCl, about 10% loss of activity
50
-
20 min, isoenzyme GlcDH-IWG3, without NaCl, complete loss of activity, isoenzymes GlcDH-II and GlcDH-IWG3 are stable in presence of 2 M NaCl
50
-
pH 6.5, 20 min, complete inactivation of wild-type enzyme, mutant enzyme E96K, E96G, E96A, Q252L and Y253C are stable
50
GlcDH-I1 is the most resistant isozyme against heat inactivation at 50°C (pH 6.5)
55
-
90% activity lost after 5 h in soluble native enzyme and GlcDHCys44 mutant enzyme, immobilized GlcDHCys44 mutant enyzyme loses 20% activity
55
-
pH 6.5, 20 min, about 65% loss of acticity of mutant enzyme Y253C, about 20% loss of activity of mutant enzyme Y252L, mutant enzyme E96K, E96G and E96A are stable
60
the enzyme shows half-lives of 23100, 364.5, and 5.3 min after 10 min at 30°C, 40°C, and 50°C, respectively. The enzyme is inactive after 10 min at 60°C
60
-
pH 6.5, 20 min, complete loss of activity of enzyme Y253C about 95% loss of activity of mutant enzyme Y252L, about 30% loss of activity of mutant enzyme E96G, mutant enzymes E96K and E95A are stable
65
-
half-life of wild-type, 8.7 min, of mutant K252L, 7.1 min, of mutant E170L/K252L, 4.2 min
65
-
half-life of wild-type, below 0.05 min, of mutant E170K 8.7 min, of mutant Q252L, 0.5 min, of mutant E170K/Q252L, 5323 min
65
-
20 min, in presence of 2 M NaCl, isoenzyme GlcDH-III loses about 30% of initial activity, isoenzyme GlcDH-IV loses about 10% of initial activity, isoenzyme IWG2 is stable
65
-
pH 6.5, 20 min, complete loss of activity of mutant enzyme Q252L, about 50% loss of activity of mutant enzyme E96K, about 10% loss of activity of mutant enzyme E96A
additional information
-
thermostability is highly increased by addition of NaCl
