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80°C, anion-exchange chromatography, hydrophobic chromatography, gel filtration with 1 mM NADP+
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ammonium sulfate, acetic acid pH 5.5, Sephadex G200, G150, crystallization
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ammonium sulfate, DEAE-Sepharose, Blue Fractogel
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ammonium sulfate, hydroxyapatite, Sephacryl S-200
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ammonium sulfate, Sephacryl S-300, cation column, affinity column
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ammonium sulfate, Sepharose 6B, DEAE-Sepharose, hydroxyapatite chromatography
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ammonium sulfate, SP-Sephadex, Phenyl-Sepharose, Affi-Gel Blue
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calcium-phosphate gel, ammonium sulfate, DEAE-cellulose
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DEAE-cellulose, partially purified enzyme, 10 mM NAD(P)+ protects from inactivation on column
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DEAE-Sephadex A-50 gel filtration
DEAE-Sephadex, Blue-Dextran Sepharose, DEAE-Sephadex, crystallization
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DEAE-Sepharose CL-6B column chromatography
DEAE-Sepharose, 2',5'-ADP-Sepharose, Sephacryl
expressed in Escherichia coli, introduced cystein residue at position 44, ammonium sulfate, phenyl-Sepharose, thiopropyl-Sepharose
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gel filtration on Superdex 75 26/60, anion exchange chromatography, and affinity chromatography using Reactive Red-500 dye affinity medium and an NaCl gradient of 0 to 1.5 M
gel filtration, DEAE-cellulose, hydroxylapatite, omega-aminohexyl-agarose
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high-pressure liquid chromatography
hydroxyapatite, QAE-Sephadex
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immobilized metal affinity chromatography
immobilized metal ion affinity chromatography (Ni2+)
methanol extraction, Mono Q, Matrex Gel Red A, Mono P, gel filtration
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native enzyme by gel filtration, recombinant enzyme from Escherichia coli by heat denaturation, and affinity gel chromatography
Ni-NTA agarose gel filtration
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Ni-NTA column chromatography
PrepEase histidine-tagged protein purification kits
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recombinant catalytic alpha-subunit from Escherichia coli
recombinant enzyme from Escherichia coli
recombinant enzyme from Escherichia coli by heat treament and 2 chromatographical steps
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recombinant enzyme is solubilized in reverse micelles of hexadecyltrimethylammonium bromide, i.e. CTAB, in cyclohexane with n-butanol as cosurfactant
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recombinant wild-type and mutant enzymes from Escherichia coli
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Sephadex G-100 gel filtration
streptomycin treatment, ammonium sulfate, DEAE-cellulose, gel filtration, DE 52 cellulose, hydroxyl apatite, gel filtration
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Triton X-100, acid treatment, ammonium sulfate, Sephacryl S-200, Sephacryl S-300, DEAE-cellulose, Sephacryl S-300
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Triton X-114, acid treatment, ammonium sulfate, Sephacryl S-200, Sephacryl S-300, SE-cellulose, DEAE-cellulose, purified enzyme contains 1.7% lipid
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DEAE-Sepharose, 2',5'-ADP-Sepharose, Sephacryl
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DEAE-Sepharose, 2',5'-ADP-Sepharose, Sephacryl
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recombinant enzyme from Escherichia coli
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recombinant enzyme from Escherichia coli
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