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1.1.1.40: malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)

This is an abbreviated version!
For detailed information about malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+), go to the full flat file.

Word Map on EC 1.1.1.40

Reaction

(S)-malate
+
NADP+
=
pyruvate
 +
CO2
 +
NADPH
 +
H+

Synonyms

ADP-malic enzyme2, c-NADP-ME, C4 NADP-malic enzyme, C4 photosynthetic NADP-malic enzyme, C4-NADP-malic enzyme, C4-NADP-ME, ChlME1, ChlME2, cNAD-ME, cytoNADPME, cytosolic malic enzyme, cytosolic NADP+-dependent isoform, cytosolic NADP+-dependent malic enzyme, L-malate: NADP oxidoreductase (decarboxylating), L-malate: NADP oxidoreductase [OAA decarboxylating], L-malate: NADP oxidoreductase [oxaloacetate decarboxylating], L-malate:NADP oxidoreductase, L-malate:NADP oxidoreductase (oxaloacetate decarboxylating), m-NAD(P)-ME, m-NADP-ME, MaeB, MaeB1, MalA, malate dehydrogenase (decarboxylating, NADP), malate dehydrogenase (NADP, decarboxylating), malE1, malic enzyme, malic enzyme 1, malic enzyme 2, malic enzyme 3, malic enzyme-NADP, ME, ME-61, ME-70, ME-NADP, ME1, ME2, ME3, mitochondrial malic enzyme, mitochondrial NADP malic enzyme, mNAD-ME, NAD(P)+-malic enzyme, NADP dependent malic enzyme, NADP malic enzyme, NADP(+)-dependent mitochondrial malic enzyme 2, NADP(H)-dependent malic enzyme, NADP+ dependent malic enzyme, NADP+-dependent decarboxylating malate dehydrogenase, NADP+-dependent malic enzyme, NADP+-dependent malic enzyme 3, NADP+-dependent ME, NADP+-ME, NADP-dependent malate dehydrogenase, NADP-dependent malic enzyme, NADP-dependent malic enzyme 1, NADP-dependent ME, NADP-linked decarboxylating malic enzyme, NADP-malate dehydrogenase, NADP-malate enzyme, NADP-malic enzyme, NADP-malic enzyme 1, NADP-malic enzyme 2, NADP-MDH, NADP-ME, NADP-ME1, NADP-ME2, NADP-ME3, NADP-ME4, NADP-specific malate dehydrogenase, NADP-specific malic enzyme, NADP-specific ME, NADPH-dependent malic enzyme, NADPH-dependent malic enzyme 1, NADPH-dependent ME1, nicotinamide adenine dinucleotide phosphate-dependent malic enzyme, nicotinamide adenine dinucleotide phosphate-malic enzyme, nonC4-NADP-ME, pNAD-ME, pyruvic-malic carboxylase, RHA1_RS44255, TME

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.40 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)

Inhibitors

Inhibitors on EC 1.1.1.40 - malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
-
at high concentrations
(S)-malate
1-methylenecyclopropan-trans-2,3-dicarboxylic acid
-
1-methylenecyclopropane
inhibits at 10 mM
2'-AMP
-
competitive
2,3-Butanedione
Pigeon
-
pseudo-first-order loss of oxidative decarboxylase activity
2-Ketoglutarate
-
34% inhibition at 2 mM
2-mercaptoethanol
-
inactivation in absence of Mg2+
2-oxoglutarate
3-(pyridin-2-yl)-6-[(5,6,7,8-tetrahydronaphthalen-2-yl)methyl][1,2,4]triazolo[3,4-b][1,3,4]thiadiazole
-
ATR7-010
5'-AMP
-
competitive
acetyl phosphate
-
slight inhibition at 2 mM
acetyl-CoA
adenosine 2'-phosphate
Pigeon
-
-
arsenite
Pigeon
-
weak
Cd2+
-
in the presence of Mn2+, Cd2+ ions almost completely inhibit the enzyme activity (5.9% residual activity)
citrate
Citric acid
-
inhibits all PtNADP-ME activities significantly
CO2
-
product inhibition, uncompetitive with respect to L-malate and NADP+, 39% inhibition at 25 mM
Cu2+-ascorbate
-
rapid inactivation by generation of reactive oxygen species at pH 5.0, Fe2+ can substitute for Cu2+, Cu2+ or ascorbate alone are not effective, azide, 1,4-diazabicyclo-(2.2.2.)octane, histidine and imidazole protect against inhibition, the substrates L-malate and NADP+ and EDTA protect almost completely, loss of activity is accompanied with cleavage of the protein into 4 fragments of 14-55 kDa
CuCl2
about 40% loss of activity within 60 min
D-fructose
-
PtNADP-ME1
D-fructose-1,6-bisphosphate
D-glucose 6-phosphate
40% inhibition of isozyme NADP-ME1 at 2 mM; inhibition of isozyme NADP-ME2
D-Glucose-6-phosphate
-
Diamide
2 mM, time-dependent decrease in activity reaching about 40% of initial activity after 60 min. In presence of dithiothreitol, a complete recovery is observed after 90 min. Enzyme oxidation decreases the catalytic activity. No severe loss of protein secondary structure takes place after oxidation; about 40% loss of activity within 60 min
Diethylamine NONOate
-
35% inhibition at 5 mM
diphenyliodonium chloride
Pigeon
-
weak
diphosphate
-
diphosphate at 2 mM inhibits the enzyme by 50%, but the activity is completely recovered if Mg2+ concentration increases to 5 mM
EDTA
-
-
embonic acid
-
-
Fe2+-ascorbate
-
rapid inactivation
fumarate
GDP
-
13% inhibition at 5 mM
glutamine
-
slight inhibition at 5 mM
Glutarate
inhibits at 10 mM
glutathione
-
strongly inactivates in absence of Mg2+
glyoxylate
H2O2
-
83% inhibition at 0.25 mM, 91.1% inhibition at 0.5 mM
Hg2+
Pigeon
-
0.0005 mM, almost complete inhibition
Hydroxypyruvate
-
hydroxypyruvate at 1 mM inhibits the enzyme activity by 45%
iodoacetate
Pigeon
-
weak
Iodosobenzoate
1 mM, time-dependent decrease in activity reaching about 40% of initial activity after 60 min; about 40% loss of activity within 60 min
L-aspartate
-
competitive, 94% inhibition at 10 mM
L-malate
pH 7.0, high concentration
Magnaporthe oryzae effector AVR-Pii
-
-
-
malate
Maleate
-
the cis isomer of fumarate, inhibition of isozyme NADP-ME2
malonate
malonyl-CoA
-
inhibition of mitochondrial enzyme and cytosolic enzyme to a much lower extent than with acetyl-CoA
Na2S
-
the enzyme activity is inhibited by up to 29-32% using 2 and 5 mM Na2S as H2S donor
NAD+
Pigeon
-
weak
NADP+
-
substrate inhibition
NADPH
-
product inhibition, competitive with respect to L-malate and NADP+
Ni2+
-
about 50% activity at 0.5 mM
NPD389
-
-
o-Iodosobenzoate
Pigeon
-
strong
oxalate
oxaloacetate
oxaloacetic acid
p-mercuribenzoate
Pigeon
-
strong
peroxynitrite
-
peroxynitrite inhibits cytosolic NADP-ME2 activity due to tyrosine nitration at Tyr-73 to 3-nitrotyrosine
Phenylglyoxal
Pigeon
-
pseudo-first-order loss of oxidative decarboxylase activity
phosphate
-
35% inhibition at 5 mM
phosphoenolpyruvate
pyruvate
S-nitrosocysteine
-
35% inhibition at 5 mM
sesamol
Sn2+
-
1 mM Sn2+ ions reduce the enzyme activity by 31%
SO32-
-
in decarboxylation of malate: partially competitive with respect to malate, in carboxylation of pyruvate: fully competitive for CO2 or HCO3-
succinate
sulfite
-
-
Tartrate
inhibits at 10 mM
Tartronate
Trypsin
-
digests the mutant enzymes, while the wild-type enzyme is protected in the presence of Mn2+, because a specific cutting site in the Lys352-Gly-Arg354 region is able to generate a unique polypeptide with Mr of 37 kDa, and this polypeptide is resistant to further digestion
-
Urea
-
inactivation at 3-5 M urea, the pigeon cytosolic NADP+-dependent malic enzyme unfolds and aggregates into various forms with dimers as the basic unit, under the same denaturing conditions but in the presence of 4 mM Mn2+, the enzyme exists exclusively as a molten globule dimer in solution, overview
additional information
-