1.1.1.391: 3beta-hydroxycholanate 3-dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about 3beta-hydroxycholanate 3-dehydrogenase (NAD+), go to the full flat file.

Reaction

Synonyms

3beta-HSD, 3beta-HSDH, 3beta-hydroxysteroid dehydrogenase, bile acid 3beta-HSD, iso bile acid dehydrogenase, NAD-dependent 3beta-hydroxysteroid dehydrogenase

ECTree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.1.391 3beta-hydroxycholanate 3-dehydrogenase (NAD+)

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Results	in table
50	AA Sequence
3	Cloned(Commentary)
5	Cofactor
2	Expression
5	General Information
2	General Stability
2	Inhibitors
4	kcat/KM [mM/s]
7	KM Value [mM]
5	Localization
2	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
9	Organism
3	Pathway
4	pH Optimum
3	Purification (Commentary)
1	Reaction
7	Reference
3	Source Tissue
9	Specific Activity [micromol/min/mg]
1	Storage Stability
25	Substrates and Products (Substrate)
2	Subunits
17	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
4	Temperature Stability [°C]
6	Turnover Number [1/s]

Purification

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Purification on EC 1.1.1.391 - 3beta-hydroxycholanate 3-dehydrogenase (NAD+)

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native enzyme 6.3fold by anion exchange chromatography and gel filtration

[Clostridium] innocuum

735471

native enzyme by anion exchange chromatography and gel filtration

Blautia producta

286228

recombinant class I ADH g2g2 isozymes from Escherichia coli by anion exchange and 5'-AMP-affinity chromatography

Homo sapiens

736216