1.1.1.337: L-2-hydroxycarboxylate dehydrogenase (NAD+)

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For detailed information about L-2-hydroxycarboxylate dehydrogenase (NAD+), go to the full flat file.

Word Map on EC 1.1.1.337

1.1.1.337

lactobacillus

confusus

dehydrogenases

l-lactate

nadh-dependent

jannaschii

sulfite

2-oxoisocaproate

cysteate

desulfonation

dogfish

methanogen

methanocaldococcus

synthesis

Reaction

Synonyms

(R)-sulfolactate dehydrogenase, ComC, L-2-hydroxyacid dehydrogenase, L-2-hydroxyacid dehydrogenase (NAD+), L-2-hydroxyisocaproate dehydrogenase, L-HicDH, L-hydroxyisocaproate dehydrogenase, L-sulfolactate dehydrogenase, MDH I, MJ1425, sulfolactate dehydrogenase

ECTree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.1.337 L-2-hydroxycarboxylate dehydrogenase (NAD+)

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Results	in table
36	AA Sequence
1	Application
4	Cloned(Commentary)
10	Cofactor
2	Crystallization (Commentary)
9	General Information
8	Inhibitors
91	kcat/KM [mM/s]
68	KM Value [mM]
2	Metals/Ions
5	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
16	Organism
6	Pathway
4	pH Optimum
2	pH Stability
1	pI Value
18	Protein Variants
3	Purification (Commentary)
1	Reaction
16	Reference
1	Source Tissue
1	Specific Activity [micromol/min/mg]
1	Storage Stability
61	Substrates and Products (Substrate)
3	Subunits
27	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Range [°C]
3	Temperature Stability [°C]
91	Turnover Number [1/s]

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Cloned on EC 1.1.1.337 - L-2-hydroxycarboxylate dehydrogenase (NAD+)

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expression in Escherichia coli

2 entries

high level expression of hexahistidine-tagged enzyme

Weissella confusa

P14295

728733

the L-HicDH gene is expressed under control of phage lambda Leftward and rightward promoters in Escherichia coli up to 35% of total cell protein. The enzyme produced under these conditions exhibits full specific activity and is found exclusively in soluble form

Weissella confusa

P14295

727592