1.1.1.31: 3-hydroxyisobutyrate dehydrogenase

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For detailed information about 3-hydroxyisobutyrate dehydrogenase, go to the full flat file.

Reaction

Synonyms

3-HIBADH, 3-hydroxyisobutyrate dehydrogenase, 3-hydroxyisobutyrate dehydrogenase-I, 3-hydroxyisobutyric acid dehydrogenase, 3HIBDH-IV, 3HIBDHI, At4g20930, AtHDH1, beta-hydroxyisobutyrate dehydrogenase, HDH1, HIBADH, HIBDH

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.31 3-hydroxyisobutyrate dehydrogenase

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Results	in table
7	Activating Compound
13566	AA Sequence
2	Application
1	CAS Registry Number
11	Cloned(Commentary)
25	Cofactor
2	Crystallization (Commentary)
12	Disease/ Diagnostics
2	Expression
9	General Information
35	Inhibitors
24	kcat/KM [mM/s]
60	KM Value [mM]
4	Localization
6	Metals/Ions
18	Molecular Weight [Da]
34	Natural Substrates/ Products (Substrates)
67	Organism
5	Pathway
49	PDB ID
14	pH Optimum
9	pH Range
3	pH Stability
14	Protein Variants
18	Purification (Commentary)
1	Reaction
3	Reaction Type
45	Reference
27	Source Tissue
13	Specific Activity [micromol/min/mg]
1	Storage Stability
101	Substrates and Products (Substrate)
19	Subunits
23	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
5	Temperature Range [°C]
3	Temperature Stability [°C]
27	Turnover Number [1/s]

Crystallization

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Crystallization on EC 1.1.1.31 - 3-hydroxyisobutyrate dehydrogenase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

powdered ammonium sulfate is added to the enzyme solution until it becomes slightly turbid, after 4 days crystallization is complete

Diutina rugosa

-

286547

native enzyme, the holoenzyme, binary complexes with NAD+, (S)-3-hydroxyisobutyrate, (R)-3-hydroxyisobutyrate, L-serine and 3-hydroxypropanoic acid and ternary complexes involving the substrates and NAD+, microbatch-under-oil method, using 0.02 M MgCl2, 0.1 M HEPES (pH 7.5), 22% (w/v) polyacrylic acid sodium salt 5100

Mycobacterium tuberculosis

P9WNY5

760528