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1.1.1.3: homoserine dehydrogenase

This is an abbreviated version!
For detailed information about homoserine dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.3

Reaction

L-homoserine
+
NAD(P)+
=
L-aspartate 4-semialdehyde
 +
NAD(P)H
 +
H+

Synonyms

AK-HDH, AK-HSD-1, AK-HSDH, AK-HseDH, aspartate kinase-homoserine dehydrogenase, aspartokinase-homoserine dehydrogenase I, bifunctional aspartate kinase-homoserine dehydrogenase, BsHSD, HDH, hom, hom-1, Hom6, homoserine dehydrogenase 1, homoserine dehydrogenase 2, HSD, HSDH, HseDH, More, orf19.2951, PbHSD, SACOL1362, StHSD, thrA, TM_0547, TTHA0489, TtHSD

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.3 homoserine dehydrogenase

Purification

Purification on EC 1.1.1.3 - homoserine dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme partially from seeds by ammonium sulfate fractionation and desalting gel filtration
-
overexpressed in Escherichia coli
recombinant enzyme
recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography, dialysis, and ultrafiltration
recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment at 70°C for 3 h, anion exchange chromatography, dialysis, and ultrafiltration
recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment, hydrophobic interaction chromatography, gel filtration, hydroxyapatite chromatography, and ultrafiltration
recombinant enzyme from Escherichia coli strain Rosetta(DE3)pLys by heat treatment at 70°C for 10 min, gel filtration, hydrophobic interaction and anion exchange chromatography, desalting gel filtration, and ultrafiltration, to homogeneity
recombinant His-tagged enzyme from Echerichia coli strain Rosetta (DE3) pLysS by nickel affinty chromatography and gel filtration
recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) to homogeneity by nickel affinity chromatography and two different steps of gel filtration
recombinant His-tagged wild-type and mutant enzymes from Echerichia coli strain Rosetta (DE3) pLysS by nickel affinty chromatography and gel filtration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) codon plus-RIPL by heat treatment at 90°C for 10 min, nickel affinity chromatography, dialysis, gel filtration, and ultrafiltration
recombinant separated catalytic domains
-
separation of the two isozymes using Blue-Sepharose chromatography
-
separation of the two isozymes using Matrex Gel Red A affinity chromatography
-
threonine sensitive and threonine insensitive isozymes
-