1.1.1.284: S-(hydroxymethyl)glutathione dehydrogenase

This is an abbreviated version!
For detailed information about S-(hydroxymethyl)glutathione dehydrogenase, go to the full flat file.

Reaction

Synonyms

ADH III, ADH3, ADH4, ADH5, AdhC, alcohol dehydrogenase 3, alcohol dehydrogenase 5, alcohol dehydrogenase class III, alcohol dehydrogenase class-3, Alcohol dehydrogenase SFA, AN7632.2, AtGSNOR, c-ADH, carbonyl reductase 1, CBR1, class III ADH, class III alcohol dehydrogenase, dehydrogenase, formaldehyde, EC 1.2.1.1, FALDH, FDH, FLD, FldA, formaldehyde dehydrogenase, formaldehyde dehydrogenase (glutathione), formic dehydrogenase, FrxA, GD-FAlDH, GFD, glutathione (GSH)-dependent formaldehyde dehydrogenase, glutathione-dependent alcohol dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase, GS-FDH, GSH-FDH, GSNO reductase, GSNO-R, GSNOR, GSNOR1, HMGSH dehydrogenase, HOT5, MGG_06011, NAD- and glutathione-dependent formaldehyde dehydrogenase, NAD-dependent formaldehyde dehydrogenase, NAD-linked formaldehyde dehydrogenase, NADH-GSNO oxidoreductase, nitroreductase, NTRA, Os02g0815500, Os02G57040, OsGSNOR, PmFLD1, PmGSNOR, S-nitrosoglutathione reductase, SA0UHSC_00833, SFA1, SlGSNOR, SoGSNOR, Tc-GFD

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.284 S-(hydroxymethyl)glutathione dehydrogenase

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Results	in table
8	Activating Compound
17026	AA Sequence
17	Application
1	CAS Registry Number
39	Cloned(Commentary)
216	Cofactor
8	Crystallization (Commentary)
782	Disease/ Diagnostics
49	Expression
209	General Information
6	General Stability
16	IC50 Value
176	Inhibitors
10	kcat/KM [mM/s]
17	Ki Value [mM]
131	KM Value [mM]
51	Localization
38	Metals/Ions
41	Molecular Weight [Da]
139	Natural Substrates/ Products (Substrates)
385	Organism
7	Pathway
30	PDB ID
52	pH Optimum
5	pH Range
8	pH Stability
1	pI Value
3	Posttranslational Modification
45	Protein Variants
37	Purification (Commentary)
8	Reaction
7	Reaction Type
206	Reference
189	Source Tissue
35	Specific Activity [micromol/min/mg]
10	Storage Stability
366	Substrates and Products (Substrate)
55	Subunits
343	Synonyms
1	Systematic Name
27	Temperature Optimum [°C]
2	Temperature Range [°C]
16	Temperature Stability [°C]
53	Turnover Number [1/s]

KM Value

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KM Value on EC 1.1.1.284 - S-(hydroxymethyl)glutathione dehydrogenase

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.041 - 4.7

12-Hydroxydodecanoic acid

4 entries

0.0509

3-acetylpyridine-adenine dinucleotide

Homo sapiens

-

-

287874

0.0626

3-nitrotyrosine

Helicobacter pylori

-

pH 7.5, 37°C

740432

0.0048

beta-NAD+

Homo sapiens

-

-

287874

3.5 - 22

cinnamyl alcohol

2 entries

0.003 - 0.0077

farnesol

2 entries

0.0071 - 1.14

formaldehyde

12 entries

0.8

geraniol

Arabidopsis thaliana

-

pH 10, 25°C

657054

0.006 - 0.48

glutathione

7 entries

0.125

glutathione monomethyl ester

Rattus norvegicus

-

-

287897

1.2 - 2.8

methylglyoxal

2 entries

0.0224

metronidazole

Helicobacter pylori

-

pH 7.5, 37°C

740432

0.0033 - 0.31

NAD+

25 entries

0.0002 - 0.25

NADH

11 entries

0.57 - 9.5

NADP+

2 entries

0.045

NADPH

Homo sapiens

-

pH 5.7

287870, 287888

0.0352

nicotinamide-hypoxanthine dinucleotide

Homo sapiens

-

-

287874

0.0423

Nitrofurantoin

Helicobacter pylori

-

pH 7.5, 37°C

740432

0.0143

nitrofurazone

Helicobacter pylori

-

pH 7.5, 37°C

740432

4

pyruvylglutathione

Homo sapiens

-

pH 5.7, with NADH as cofactor

287888

0.16

reduced glutathione

Komagataella pastoris

-

-

287867

0.058 - 1.2

S-(hydroxymethyl)glutathione

5 entries

0.12 - 0.6

S-formylglutathione

4 entries

0.0004 - 0.094

S-hydroxymethyl glutathione

7 entries

0.00012 - 2.65

S-hydroxymethylglutathione

8 entries

0.0072

S-nitrosocysteine

Helicobacter pylori

-

pH 7.5, 37°C

740432

0.0041 - 0.55

S-nitrosoglutathione

16 entries

0.057

S-nitrosylglutathione

Solanum lycopersicum

D2Y3F4

pH 8.0, 30°C, recombinant enzyme

724489

0.0301

thio-NAD+

Homo sapiens

-

-

287874

additional information

additional information

2 entries

-

0.041

12-Hydroxydodecanoic acid

Homo sapiens

-

wild type enzyme, pH 10

667724

0.16

12-Hydroxydodecanoic acid

Homo sapiens

-

mutant R368L, pH 10

667724

0.68

12-Hydroxydodecanoic acid

Homo sapiens

-

mutant E67L, pH 10

667724

4.7

12-Hydroxydodecanoic acid

Arabidopsis thaliana

-

pH 10, 25°C

657054

3.5

cinnamyl alcohol

Arabidopsis thaliana

-

pH 10, 25°C

657054

22

cinnamyl alcohol

Arabidopsis thaliana

-

pH 7.5, 25°C

657054

0.003

farnesol

Arabidopsis thaliana

-

pH 10, 25°C

657054

0.0077

farnesol

Arabidopsis thaliana

-

pH 7.5, 25°C

657054

0.0071

formaldehyde

Rattus norvegicus

-

-

287866

0.0087

formaldehyde

Homo sapiens

-

-

287866

0.009

formaldehyde

Homo sapiens

-

pH 8

287870

0.019

formaldehyde

Canis lupus familiaris

-

-

287891

0.02

formaldehyde

Zea mays

-

-

287891

0.021

formaldehyde

Zea mays

-

-

287891

0.25

formaldehyde

[Candida] boidinii

-

-

287869, 287889

0.29

formaldehyde

[Candida] boidinii

-

-

287862

0.31

formaldehyde

Komagataella pastoris

-

-

287867

0.39

formaldehyde

Pseudomonas putida

-

-

287885

0.43

formaldehyde

Komagataella pastoris

-

-

80989

1.14

formaldehyde

Schwanniomyces vanrijiae

-

-

287885

0.006

glutathione

Rattus norvegicus

-

-

287897

0.008

glutathione

Homo sapiens

-

pH 8

287870

0.0081

glutathione

Homo sapiens

-

pH 8.0

287888

0.037

glutathione

Zea mays

-

-

287891

0.062

glutathione

Canis lupus familiaris

-

-

287891

0.13

glutathione

[Candida] boidinii

-

-

287869, 287889

0.48

glutathione

Komagataella pastoris

-

-

80989

1.2

methylglyoxal

[Candida] boidinii

-

-

287869, 287889

2.8

methylglyoxal

[Candida] boidinii

-

-

287862

0.0033

NAD+

Homo sapiens

-

wild type enzyme

667724

0.0065

NAD+

Pisum sativum

-

reaction with S-hydroxymethyl glutathione

287880

0.007

NAD+

Homo sapiens

-

-

287866

0.008

NAD+

Branchiostoma floridae

-

in 0.1 M sodium diphosphate at pH 7.5

668977

0.009

NAD+

Homo sapiens

-

pH 8.0

287888

0.0093

NAD+

Homo sapiens

-

wild type enzyme, pH 10

667724

0.01

NAD+

Rattus norvegicus

-

-

287866

0.0136

NAD+

Homo sapiens

-

pH 5.7

287888

0.0144

NAD+

Homo sapiens

-

mutant R368L, pH 7.5

667724

0.025

NAD+

[Candida] boidinii

-

-

287862

0.031

NAD+

Zea mays

-

-

287891

0.031

NAD+

Canis lupus familiaris

-

-

287891

0.04

NAD+

Solanum lycopersicum

D2Y3F4

pH 8.0, 30°C, recombinant enzyme

724489

0.045

NAD+

Saccharomyces cerevisiae

-

wilde-type enzyme

287894

0.05

NAD+

[Candida] boidinii

-

-

287879

0.054

NAD+

Homo sapiens

-

mutant E67L, pH 10

667724

0.068

NAD+

Saccharomyces cerevisiae

-

-

287864

0.09

NAD+

[Candida] boidinii

-

-

287869, 287889

0.116

NAD+

Homo sapiens

-

mutant E67L

667724

0.12

NAD+

Komagataella pastoris

-

-

287867

0.14

NAD+

Pisum sativum

-

reaction with S-hydroxymethyl glutathione

287880

0.15

NAD+

Pseudomonas putida

-

-

287885

0.16

NAD+

Homo sapiens

-

mutant R368L, pH 10

667724

0.24

NAD+

Komagataella pastoris

-

-

80989

0.31

NAD+

Schwanniomyces vanrijiae

-

-

287885

0.0002

NADH

Homo sapiens

-

wild type enzyme, pH 7.5

667724

0.0042

NADH

Homo sapiens

-

pH 5.7

287870, 287888

0.011

NADH

Homo sapiens

-

mutant R368L, pH 7.5

667724

0.0143

NADH

Chlamydomonas reinhardtii

-

pH 7.9, temperature not specified in the publication

763680

0.016

NADH

Homo sapiens

-

pH 8.0

287888

0.025

NADH

[Candida] boidinii

-

-

287862

0.045

NADH

Branchiostoma floridae

-

in 0.1 M sodium diphosphate at pH 7.5

668977

0.054

NADH

Brassica oleracea var. botrytis

J7GP93

pH 8.0, 30°C

741071

0.058

NADH

Solanum lycopersicum

D2Y3F4

pH 8.0, 30°C, recombinant enzyme

724489

0.064

NADH

Lactuca sativa

J7GHV7

pH 8.0, 30°C

741071

0.25

NADH

Komagataella pastoris

-

-

287867

0.57

NADP+

Homo sapiens

-

pH 5.7

287888

9.5

NADP+

Homo sapiens

-

pH 8.0

287888

0.058

S-(hydroxymethyl)glutathione

Solanum lycopersicum

D2Y3F4

pH 8.0, 30°C, recombinant enzyme

724489

0.058

S-(hydroxymethyl)glutathione

Solanum lycopersicum

D2Y3F4

pH and temperature not specified in the publication

763605

0.066

S-(hydroxymethyl)glutathione

Brassica oleracea var. botrytis

J7GP93

pH 8.0, 30°C

741071

0.066

S-(hydroxymethyl)glutathione

Lactuca sativa

J7GHV7

pH 8.0, 30°C

741071

1.2

S-(hydroxymethyl)glutathione

Taiwanofungus camphoratus

D2CFJ2

pH 8.0, 25°C

703794

0.12

S-formylglutathione

[Candida] boidinii

-

-

287862

0.28

S-formylglutathione

Homo sapiens

-

pH 5.7, with NADPH as cofactor

287888

0.3

S-formylglutathione

Homo sapiens

-

pH 5.7, with NADH as cofactor

287870, 287888

0.6

S-formylglutathione

Komagataella pastoris

-

-

287867

0.0004

S-hydroxymethyl glutathione

Homo sapiens

-

-

287880

0.00092

S-hydroxymethyl glutathione

Rattus norvegicus

-

-

287880

0.002

S-hydroxymethyl glutathione

Pisum sativum

-

-

287880

0.0025

S-hydroxymethyl glutathione

Saccharomyces cerevisiae

-

mutant D267E/T269I

287894

0.006

S-hydroxymethyl glutathione

Drosophila melanogaster

-

-

287880

0.04

S-hydroxymethyl glutathione

Saccharomyces cerevisiae

-

-

287880

0.094

S-hydroxymethyl glutathione

Escherichia coli

-

-

287880

0.00012

S-hydroxymethylglutathione

Homo sapiens

-

in 0.1 M potassium phosphate (pH 7.5), at 25°C

696092

0.0017

S-hydroxymethylglutathione

Homo sapiens

-

wild type enzyme, pH 7.5, 30°C

667724

0.004

S-hydroxymethylglutathione

Branchiostoma floridae

-

in 0.1 M sodium diphosphate at pH 8.0

668977

0.007

S-hydroxymethylglutathione

Arabidopsis thaliana

-

pH 8, 25°C

657054

0.008

S-hydroxymethylglutathione

Bos taurus

-

-

287890

0.03

S-hydroxymethylglutathione

Saccharomyces cerevisiae

-

wild-type enzyme

287894

0.038

S-hydroxymethylglutathione

Homo sapiens

-

mutant R368L, pH 7.5, 30°C

667724

2.65

S-hydroxymethylglutathione

Homo sapiens

-

mutant E67L, pH 7.5, 30°C

667724

0.0041

S-nitrosoglutathione

Helicobacter pylori

-

pH 7.5, 37°C

740432

0.0048

S-nitrosoglutathione

Homo sapiens

-

wild type enzyme, pH 7.5, 25°C

667724

0.011

S-nitrosoglutathione

Homo sapiens

-

-

697214

0.0111

S-nitrosoglutathione

Homo sapiens

-

in 0.1 M potassium phosphate (pH 7.5), at 25°C

696092

0.0113

S-nitrosoglutathione

Homo sapiens

P11766

wild-type enzyme, pH and temperature not specified in the publication

762616

0.0249

S-nitrosoglutathione

Chlamydomonas reinhardtii

-

pH 7.9, temperature not specified in the publication

763680

0.0284

S-nitrosoglutathione

Homo sapiens

P11766

mutant K188A, pH and temperature not specified in the publication

762616

0.0301

S-nitrosoglutathione

Homo sapiens

-

-

704451

0.045

S-nitrosoglutathione

Lactuca sativa

J7GHV7

pH 8.0, 30°C

741071

0.053

S-nitrosoglutathione

Brassica oleracea var. botrytis

J7GP93

pH 8.0, 30°C

741071

0.056

S-nitrosoglutathione

Branchiostoma floridae

-

in 0.1 M sodium diphosphate at pH 8.0

668977

0.057

S-nitrosoglutathione

Solanum lycopersicum

D2Y3F4

pH and temperature not specified in the publication

763605

0.16

S-nitrosoglutathione

Homo sapiens

-

mutant R368L, pH 7.5, 25°C

667724

0.1806

S-nitrosoglutathione

Staphylococcus aureus

-

at pH 7.6

704293

0.28

S-nitrosoglutathione

Taiwanofungus camphoratus

D2CFJ2

pH 8.0, 25°C

703794

0.55

S-nitrosoglutathione

Aspergillus nidulans

Q5AVP8

pH 6.0, 37°C

740859

additional information

additional information

Pisum sativum

-

-

80983

-

additional information

additional information

Homo sapiens

P11766

enzyme GSNOR kinetic analysis data results in nonhyperbolic behavior that is successfully accommodated by the Hill-Langmuir equation with a Hill coefficient of +1.75, indicating that the substrate, GSNO, is acting as a positive allosteric affector. GSNOR Michaelis-Menten steady-state kinetics display allosteric behavior

762616

-