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1.1.1.284: S-(hydroxymethyl)glutathione dehydrogenase

This is an abbreviated version!
For detailed information about S-(hydroxymethyl)glutathione dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.284

Reaction

S-(hydroxymethyl)glutathione
+
NAD(P)+
=
S-formylglutathione
 +
NAD(P)H
 +
H+

Synonyms

ADH III, ADH3, ADH4, ADH5, AdhC, alcohol dehydrogenase 3, alcohol dehydrogenase 5, alcohol dehydrogenase class III, alcohol dehydrogenase class-3, Alcohol dehydrogenase SFA, AN7632.2, AtGSNOR, c-ADH, carbonyl reductase 1, CBR1, class III ADH, class III alcohol dehydrogenase, dehydrogenase, formaldehyde, EC 1.2.1.1, FALDH, FDH, FLD, FldA, formaldehyde dehydrogenase, formaldehyde dehydrogenase (glutathione), formic dehydrogenase, FrxA, GD-FAlDH, GFD, glutathione (GSH)-dependent formaldehyde dehydrogenase, glutathione-dependent alcohol dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase, GS-FDH, GSH-FDH, GSNO reductase, GSNO-R, GSNOR, GSNOR1, HMGSH dehydrogenase, HOT5, MGG_06011, NAD- and glutathione-dependent formaldehyde dehydrogenase, NAD-dependent formaldehyde dehydrogenase, NAD-linked formaldehyde dehydrogenase, NADH-GSNO oxidoreductase, nitroreductase, NTRA, Os02g0815500, Os02G57040, OsGSNOR, PmFLD1, PmGSNOR, S-nitrosoglutathione reductase, SA0UHSC_00833, SFA1, SlGSNOR, SoGSNOR, Tc-GFD

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.284 S-(hydroxymethyl)glutathione dehydrogenase

Cloned

Cloned on EC 1.1.1.284 - S-(hydroxymethyl)glutathione dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
characterization of the DNA sequence of the gfd/odh genomic region containing the gene which encodes glutathione-dependent formaldehyde dehydrogenase
-
construction of a FDHoverproducing strain by insertion of the FLD1 gene with the own promoter into the integrative plasmid pYT1 containing the LEU2 gene of Saccharomyces cerevisiae as a selective marker , multi-copy integration and expression in strain NCYC 495 (leu1-1)
-
DNA and amino acid sequence determination and analysis
DNA and amino acid sequence determination and analysis, sequence comparison, recombinant overexpression of SoGSNOR in transgenic Nicotiana tabacum cv. NC89 seedlings using the agrobacterium tumefaciens strain LBA4404 transfection system, quantitative RT-PCR enzyme expression analysis
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 (DE3) cells
-
expressed in Escherichia coli BL21 cells
-
expression in Escherichia coli
expression in HEK 293T cell
gene ADH3, quantitative PCR enzyme expression analysis
gene ADH3, recombinant expression of GFP-tagged enzyme in Solanum chmielewskii leaves, mainly in the vascular tissue and epidermal cells
gene ADH3, recombinant expression of GFP-tagged enzyme in Solanum habrochaites leaves, mainly in the vascular tissue and epidermal cells
gene ADH3, recombinant expression of GFP-tagged enzyme in Solanum lycopersicum leaves, mainly in the vascular tissue and epidermal cells
gene ADH3, recombinant overexpression of enzyme GSNOR in Solanum lycopersicum using Agrobacterium-mediated transformation of the CaMV35S::GSNOR overexpression construct, quantitative real-time PCR enzyme expression analysis
gene ADH5, quantitative reverse transcription PCR enzyme expression analysis
gene ADH5, recombinant expression of His-tagged enzyme in Escherichia coli
gene adhC, encoded in the adhC-estD operon, expression of the adhC-estD operon is regulated by NmlRHI in strain Rd KW20, the Neisseria MerR-like regulator, overview. The NmlRHI-AdhC system in the genome sequences of nontypeable Haemophilus influenzae strains R2846, R2866, and 86-028NP shows variations, the adhC gene of strain 86-028NP is nonfunctional due to a premature stop codon, polymorphisms in the operator/promoter region of strain R2866 result in reduced enzyme activity, genotyping of clinical isolates, in silico promoter analysis, overview
gene GSNOR, sequence comparisons
gene Os02g0815500 or Os02G57040, single copy gene
overexpressed in Escherichia coli
-
overexpression in Escherichia coli
-
overexpression in Hansenula polymorpha strain Tf 11-6, method optimization for recombinant large scale production, overview
-
overexpression in Saccharomyces cerevisiae
-
overexpression of the fdh in the central nervous system of transgenic Drosophila melanogaster
-
quantitative PCR enzyme expression analysis
quantitative real-time PCR enzyme expression analysis
real-time PCR expression analysis, recombinant expression in Escherichia coli as N-terminally His-tagged enzyme
recombinant expression of wild-type and mutant enzymes in HEK 293T cells, transfectioon via a v-raf/v-myc oncogene-carrying retrovirus, improvement by codon optimization. The Tc-GFD protein expressed in pcGFD-O transfected 293T cells provides protection to these cells against apoptosis under GSNO induced oxidative stress, overview
subcloned into NdeI and NotI sites of pET-20b(+) vector and overexpressed in Escherichia coli BL21(DE)pLysS
wild-type cloned into pET28a(+), yielding pET28NtrA, and transformed in Escherichia coli BL21-Gold(DE3). DELTAntrA mutant (LMSA833) ligated to pSP64D-E (pSP64-SA0833) and integrated into Staphylococcus aureus RN4220
-