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0.0073 - 10.32
(R)-lactate
4.4 - 37.2
2-oxobutanoate
3.2 - 53
2-oxoisocaproate
35
2-oxoisovalerate
pH 7.0, 30°C, recombinant enzyme
1.3 - 24.07
3,4-dihydroxyphenylpyruvic acid
0.89 - 23.2
3-methyl-2-oxobutanoate
4.2 - 140
Hydroxypyruvate
0.48 - 5.6
nicotinamide cytosine dinucleotide
0.0073
(R)-lactate
pH 6.5, 30°C, recombinant enzyme
10.32
(R)-lactate
-
pH 8, , 30°C
4.4
2-oxobutanoate
wild-type, pH 5.5, 30°C
9.48
2-oxobutanoate
-
pH 8, 30°C
16.8
2-oxobutanoate
mutant Y51L, pH 5.5, 30°C
20.7
2-oxobutanoate
mutant Y51M, pH 5.5, 30°C
37.2
2-oxobutanoate
mutant Y51F, pH 5.5, 30°C
1
2-oxobutyrate
pH 7.0, 30°C, recombinant enzyme
150
2-oxobutyrate
pH 7.0, 30°C, recombinant enzyme
440
2-oxobutyrate
pH 7.0, 30°C, recombinant enzyme
27
2-oxocaproate
pH 7.0, 30°C, recombinant enzyme
87
2-oxocaproate
pH 7.0, 30°C, recombinant enzyme
3.2
2-oxoisocaproate
pH 7.0, 30°C, recombinant enzyme
53
2-oxoisocaproate
pH 7.0, 30°C, recombinant enzyme
18
2-oxovalerate
pH 7.0, 30°C, recombinant enzyme
200
2-oxovalerate
pH 7.0, 30°C, recombinant enzyme
1.3
3,4-dihydroxyphenylpyruvic acid
-
cosubstrate NADPH, pH 8.0, 25°C
24.07
3,4-dihydroxyphenylpyruvic acid
-
cosubstrate NADH, pH 8.0, 25°C
0.89
3-methyl-2-oxobutanoate
wild-type, pH 5.5, 30°C
7.4
3-methyl-2-oxobutanoate
mutant Y51L, pH 5.5, 30°C
8.9
3-methyl-2-oxobutanoate
mutant Y51M, pH 5.5, 30°C
23.2
3-methyl-2-oxobutanoate
mutant Y51F, pH 5.5, 30°C
0.7
glyoxylate
pH 7.0, 30°C, recombinant enzyme
5.3
glyoxylate
pH 7.0, 30°C, recombinant enzyme
130
glyoxylate
pH 7.0, 30°C, recombinant enzyme
4.2
Hydroxypyruvate
pH 7.0, 30°C, recombinant enzyme
8.7
Hydroxypyruvate
pH 7.0, 30°C, recombinant enzyme
140
Hydroxypyruvate
pH 7.0, 30°C, recombinant enzyme
0.05
NAD+
mutant V152R/N213E, pH not specified in the publication, temperature not specified in the publication
0.074
NAD+
mutant V152R/I177K/N213I, pH not specified in the publication, temperature not specified in the publication
0.71
NAD+
mutant V152R/I177K, pH not specified in the publication, temperature not specified in the publication
1.41
NAD+
-
isozyme D-LDH2, pH 8.0, 25°C
1.56
NAD+
-
isozyme D-LDH3, pH 8.0, 25°C
1.61
NAD+
-
isozyme D-LDH1, pH 8.0, 25°C
2.3
NAD+
mutant V152R, pH not specified in the publication, temperature not specified in the publication
10.1
NAD+
wild-type, pH not specified in the publication, temperature not specified in the publication
56.16
NADH
-
pH 8, 50°C
182.6
NADH
-
isozyme D-LDH2, pH 8.0, 25°C
206.3
NADH
-
isozyme D-LDH1, pH 8.0, 25°C
232.6
NADH
-
isozyme D-LDH3, pH 8.0, 25°C
2500
NADH
pH 8.0, 30°C, recombinant enzyme
2800
NADH
pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate
3700
NADH
pH 8.0, 30°C, recombinant enzyme, with Mg2+
4400
NADH
pH 7.0, 30°C, recombinant enzyme
7700
NADH
pH 8.0, 30°C, recombinant enzyme
7700
NADH
pH 8.0, 30°C, recombinant enzyme, with Mg2+
7700
NADH
pH 8.0, 30°C, recombinant enzyme, with Mg2+
7800
NADH
pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate
8000
NADH
pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate
8100
NADH
pH 8.0, 30°C, recombinant enzyme
12000
NADH
pH 7.0, 30°C, recombinant enzyme
17000
NADH
pH 7.0, 30°C, recombinant enzyme
0.48
nicotinamide cytosine dinucleotide
wild-type, pH not specified in the publication, temperature not specified in the publication
2.1
nicotinamide cytosine dinucleotide
mutant V152R/N213E, pH not specified in the publication, temperature not specified in the publication
3.1
nicotinamide cytosine dinucleotide
mutant V152R/I177K/N213I, pH not specified in the publication, temperature not specified in the publication
3.3
nicotinamide cytosine dinucleotide
mutant V152R/I177K, pH not specified in the publication, temperature not specified in the publication
5.6
nicotinamide cytosine dinucleotide
mutant V152R, pH not specified in the publication, temperature not specified in the publication
19.87
oxaloacetate
-
pH 8, 30°C
21
oxaloacetate
pH 7.0, 30°C, recombinant enzyme
31
oxaloacetate
pH 7.0, 30°C, recombinant enzyme
230
oxaloacetate
pH 7.0, 30°C, recombinant enzyme
1
phenylpyruvate
-
pH 7.4, 37°C, recombinant His-taged wild-type enzyme
2.6
phenylpyruvate
pH 7.0, 30°C, recombinant enzyme
4.34
phenylpyruvate
pH 7, 30°C
5.7
phenylpyruvate
-
pH 7.4, 37°C, recombinant His-taged mutant F299Y
6 - 12
phenylpyruvate
mutant Y51S, pH 5.5, 30°C
11
phenylpyruvate
pH 7.0, 30°C, recombinant enzyme
15.9
phenylpyruvate
wild-type, pH 5.5, 30°C
73.01
phenylpyruvate
-
pH 8, 50°C
100
phenylpyruvate
-
pH 5.5, 45°C
105
phenylpyruvate
-
recombinant enzyme, pH 5.5, 30°C
1000
phenylpyruvate
-
pH 7.4, 37°C, recombinant His-taged mutant Y52L/F299Y
1720
phenylpyruvate
mutant Y51A, pH 5.5, 30°C
1880
phenylpyruvate
mutant Y51F, pH 5.5, 30°C
2130
phenylpyruvate
mutant Y51M, pH 5.5, 30°C
2200
phenylpyruvate
mutant Y51L, pH 5.5, 30°C
7500
phenylpyruvate
-
pH 7.4, 37°C, recombinant His-taged mutant Y52L
0.000002
pyruvate
-
mutant A100V, pH 7, 27°C
0.000005
pyruvate
-
mutant N263G, pH 7, 27°C
0.000006
pyruvate
-
mutant A234T, pH 7, 27°C
0.000013
pyruvate
-
mutant A234C, pH 7, 27°C
0.000021
pyruvate
-
mutant F299Y, pH 7, 27°C
0.000027
pyruvate
-
mutant T260V, pH 7, 27°C
0.000031
pyruvate
-
mutant G297A, pH 7, 27°C
0.000031
pyruvate
-
wild-type, pH 7, 27°C
0.000034
pyruvate
-
mutant A78V, pH 7, 27°C
0.000037
pyruvate
-
mutant A234G, pH 7, 27°C
0.000055
pyruvate
-
mutant T75L, pH 7, 27°C
0.00006
pyruvate
-
mutant A234S, pH 7, 27°C
0.000157
pyruvate
-
mutant T75L/A234G, pH 7, 27°C
0.000211
pyruvate
-
mutant T75L/A234S, pH 7, 27°C
1.9
pyruvate
pH 6.5, 30°C, recombinant enzyme
3.5
pyruvate
-
isoform LDHD3, pH 5.5, 35°C
32
pyruvate
-
isoform LDHD2, pH 7.5, 35°C
34
pyruvate
pH 6.5, 30°C, recombinant enzyme
35
pyruvate
pH 8.0, 30°C, recombinant enzyme
50
pyruvate
pH 8.0, 30°C, recombinant enzyme
51
pyruvate
pH 8.0, 30°C, recombinant enzyme, with Mg2+
60
pyruvate
pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate
61
pyruvate
pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate
65
pyruvate
pH 8.0, 30°C, recombinant enzyme, with Mg2+
160
pyruvate
pH 7.0, 30°C, recombinant enzyme
161
pyruvate
-
isoform LDHD1, pH 6, 35°C
200
pyruvate
pH 8.0, 30°C, recombinant enzyme
230
pyruvate
pH 7.0, 30°C, recombinant enzyme
280
pyruvate
pH 8.0, 30°C, recombinant enzyme, with fructose 1,6-bisphosphate
658
pyruvate
-
pH 5.5, 45°C
820
pyruvate
pH 8.0, 30°C, recombinant enzyme, with Mg2+
1030
pyruvate
-
pH 8, 30°C
3157
pyruvate
-
recombinant enzyme, pH 5.5, 30°C
4000
pyruvate
pH 7.0, 30°C, recombinant enzyme
7900
pyruvate
pH 6.5, 30°C, recombinant enzyme