1.1.1.274: 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming)

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For detailed information about 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming), go to the full flat file.

Word Map on EC 1.1.1.274

1.1.1.274

2,5-diketo-d-gluconic

corynebacterium

2-keto-l-gulonic

nadph-dependent

aldo-keto

l-ascorbic

d-glucose

synthesis

citrinum

penicillium

activity-stained

aldose

cofactor-binding

reductases

biocatalysis

erwinia

error-prone

stereo-specific

ascorbate

enantioselectivity

two-phase

pantoea

citrea

biotechnology

Reaction

Synonyms

2,5-diketo-D-gluconate reductase, 2,5-diketo-D-gluconic acid reductase, 2,5-diketo-gluconate reductase, 2,5-DKG, 2,5-DKG reductase, 2,5-DKGR, 2,5DKGR, beta-keto ester reductase, CTATCC11996_22452, Dkr, YafB, YqhE, YqhE reductase

ECTree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.1.274 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming)

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Results	in table
2645	AA Sequence
4	Application
1	CAS Registry Number
8	Cloned(Commentary)
16	Cofactor
3	Crystallization (Commentary)
2	Expression
7	General Information
1	General Stability
6	Inhibitors
1	Ki Value [mM]
53	KM Value [mM]
9	Molecular Weight [Da]
11	Natural Substrates/ Products (Substrates)
18	Organism
4	Pathway
4	PDB ID
7	pH Optimum
2	pH Range
24	Protein Variants
9	Purification (Commentary)
6	Reaction
3	Reaction Type
17	Reference
25	Specific Activity [micromol/min/mg]
3	Storage Stability
51	Substrates and Products (Substrate)
12	Subunits
25	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Stability [°C]
20	Turnover Number [1/s]

Natural Substrates Products

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Natural Substrates Products on EC 1.1.1.274 - 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming)

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2,5-didehydro-D-gluconate + NADPH

2-oxo-L-gulonic acid + NADP+

Corynebacterium sp.

P06632

step in the biosynthesis of L-ascorbic acid

657308

2,5-didehydro-D-gluconate + NADPH + H+

2-dehydro-D-gluconate + NADP+

Corynebacterium glutamicum

H9CWC0

723915

2-dehydro-D-gulonate + NADP+

2,5-didehydro-D-gluconate + NADPH + H+

2 entries

ethyl 2-ethylacetoacetate + NADPH

ethyl (2R)-ethyl-(3S)-hydroxybutanoate + NADP+

Escherichia coli

stereospecific reaction

440312

ethyl 2-methylacetoacetate + NADPH

ethyl (2R)-methyl-(3S)-hydroxybutanoate + NADP+

Escherichia coli

stereospecific reaction

440312

ethyl acetoacetate + NADPH

ethyl (3S)-hydroxybutanoate + NADP+

Escherichia coli

stereospecific reaction

440312

additional information

4 entries