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0.00036 - 0.42
3-dehydroquinate
0.029 - 186
3-dehydroshikimate
0.05 - 0.087
shikimic acid
additional information
additional information
-
0.00036
3-dehydroquinate
pH 7.0, 30°C
0.42
3-dehydroquinate
pH 7.0, 30°C
0.029
3-dehydroshikimate
-
-
0.029
3-dehydroshikimate
wild-type enzyme, pH 7.3, 25°C
0.031
3-dehydroshikimate
pH 7.0, 25°C, recombinant AroE
0.031
3-dehydroshikimate
-
pH 7.0, 25°C, with NADPH
0.044
3-dehydroshikimate
25°C, pH 7.3
0.046
3-dehydroshikimate
pH 7.0, 30°C, recombinant isozyme VvSDH3
0.076
3-dehydroshikimate
mutant K69A, pH 7.3, 25°C
0.157
3-dehydroshikimate
pH 7.0, 30°C, recombinant isozyme VvSDH1
0.2
3-dehydroshikimate
pH 7.0, 30°C
0.272
3-dehydroshikimate
pH 9.0, 30°C
0.272
3-dehydroshikimate
with NADPH, pH 8.6, 30°C
0.287
3-dehydroshikimate
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with NADPH
0.322
3-dehydroshikimate
pH 7.0, 30°C, EcDQD/SDH2, DQD activity
0.331
3-dehydroshikimate
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with NADPH
0.381
3-dehydroshikimate
pH 6.5, temperature not specified in the publication
0.381
3-dehydroshikimate
pH 6.5, 30°C, EcDQD/SDH1, shikimate formation
0.449
3-dehydroshikimate
pH 7.0, 30°C, EcDQD/SDH1, DQD activity
0.466
3-dehydroshikimate
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHd, with NADPH
0.878
3-dehydroshikimate
pH 6.5, temperature not specified in the publication
0.878
3-dehydroshikimate
pH 6.5, 30°C, EcDQD/SDH3, shikimate formation
0.882
3-dehydroshikimate
pH 7.0, 30°C, EcDQD/SDH3, DQD activity
8.83
3-dehydroshikimate
pH 8.5, temperature not specified in the publication
8.83
3-dehydroshikimate
pH 8.5, 30°C, EcDQD/SDH2, shikimate formation
9.44
3-dehydroshikimate
pH 10.5, 30°C, EcDQD/SDH2, gallate formation
30.6
3-dehydroshikimate
pH 10.5, 30°C, EcDQD/SDH2, gallate formation
186
3-dehydroshikimate
pH 7.0, 30°C, recombinant isozyme VvSDH4
2.351
L-quinate
-
mutant S275G/T318G, pH and temperature not specified in the publication
3.33
L-quinate
pH and temperature not specified in the publication, mutant T381G
4.075
L-quinate
pH and temperature not specified in the publication, mutant S338G/T381G
4.485
L-quinate
pH and temperature not specified in the publication, mutant T381S
5.135
L-quinate
pH and temperature not specified in the publication, mutant T381A
0.572
NAD+
pH 8.8, presence of 2 mM shikimate
1.083
NAD+
pH 8.8, presence of 2 mM quinate
2.9
NAD+
pH 8.0, 60°C, recombinant enzyme
5.4
NAD+
pH 8.8, presence of 2 mM shikimate
5.43
NAD+
pH 8.8, presence of 2 mM shikimate
5.43
NAD+
in the presence of shikimate
0.007
NADP+
-
-
0.0073
NADP+
pH 8.8, presence of 2 mM shikimate
0.0091
NADP+
-
pH 8.8, temperature not specified in the publication
0.0091
NADP+
pH 8.8, 25°C, with shikimate
0.0135
NADP+
with shikimate, pH 7.0, 25°C
0.017
NADP+
-
pH 7.5, 22°C, mutant K385A
0.022
NADP+
pH 7.0, 25°C, recombinant AroE
0.022
NADP+
-
pH 7.5, 22°C, mutant K385N/D423N
0.022
NADP+
-
pH 7.0, 25°C, with shikimate
0.023
NADP+
pH 7.0, 30°C, recombinant isozyme VvSDH3
0.0295
NADP+
-
pH 9.0, 25°C
0.038
NADP+
pH 9.0, 30°C, recombinant isozyme VvSDH3
0.0415
NADP+
pH 9.0, temperature not specified in the publication
0.0415
NADP+
pH 9.0, 30°C, EcDQD/SDH3, shikimate oxidation
0.0424
NADP+
25°C, pH 9.0
0.0424
NADP+
with shikimate, pH 9.0, 25°C
0.04255
NADP+
-
pH not specified in the publication, 25°C
0.0426
NADP+
-
pH not specified in the publication, 25°C, with shikimate
0.046
NADP+
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHD, with shikimate
0.053
NADP+
-
pH 7.5, 22°C, mutant K385N
0.0538
NADP+
pH 9.0, 30°C, recombinant isozyme VvSDH4
0.055
NADP+
pH 8.8, 25°C, with shikimate
0.056
NADP+
-
with shikimate, pH 9.0, 20°C
0.0565
NADP+
pH 9.0, temperature not specified in the publication
0.0565
NADP+
pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation
0.062
NADP+
-
mutant T381S, 22°C
0.063
NADP+
-
pH 9.0, 25°C, with shikimate
0.0652
NADP+
pH 9.0, temperature not specified in the publication
0.0652
NADP+
pH 9.0, 30°C, EcDQD/SDH2, shikimate oxidation
0.068
NADP+
-
mutant H335A, 22°C
0.079
NADP+
-
pH 7.5, 22°C, mutant D423N
0.0928
NADP+
-
mutant T381A, 22°C
0.1
NADP+
pH 7.0, 25°C, with shikimate
0.107
NADP+
-
mutant T422S, 22°C
0.112
NADP+
pH 8.8, presence of 2 mM shikimate
0.112
NADP+
in the presence of shikimate
0.114
NADP+
-
pH 7.5, 22°C, mutant S338A
0.114
NADP+
pH 9.0, 30°C, recombinant isozyme VvSDH1
0.115
NADP+
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with shikimate
0.131
NADP+
-
pH 7.5, 22°C, wild-type DELTA88DHQ-SDH enzyme variant
0.131
NADP+
-
wild-type, 22°C
0.131
NADP+
pH 8.8, 22°C, with shikimate
0.132
NADP+
-
pH 7.5, 22°C, mutant Y550F
0.138
NADP+
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with shikimate
0.152
NADP+
-
pH 7.5, 22°C, mutant S336A
0.156
NADP+
-
mutant T407A, 22°C
0.157
NADP+
-
pH 7.5, 22°C, mutant Y550A
0.159
NADP+
-
mutant Q78L, 22°C
0.182
NADP+
pH 8.0, 60°C, recombinant enzyme
0.182
NADP+
-
with shikimate, pH 8.0, 25°C
0.19
NADP+
-
87°C, pH 7.3
0.19
NADP+
with shikimate, pH 7.3, 87°C
0.217
NADP+
pH 7.0, 30°C, recombinant isozyme VvSDH1
0.00438
NADPH
pH 8.5, temperature not specified in the publication
0.00438
NADPH
pH 8.5, 30°C, EcDQD/SDH2, shikimate formation
0.01
NADPH
pH 7.0, 25°C, recombinant AroE
0.011
NADPH
wild-type enzyme, pH 7.3, 25°C
0.0231
NADPH
pH 6.5, 30°C, EcDQD/SDH3, shikimate formation
0.03
NADPH
mutant K69A, pH 7.3, 25°C
0.031
NADPH
-
pH 7.0, 25°C, with 3-dehydroshikimate
0.0495
NADPH
pH 6.5, temperature not specified in the publication
0.0495
NADPH
pH 6.5, 30°C, EcDQD/SDH1, shikimate formation
0.249
NADPH
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHD, with 3-dehydroshikimate
0.267
NADPH
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with 3-dehydroshikimate
0.27
NADPH
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with 3-dehydroshikimate
23.1
NADPH
pH 6.5, temperature not specified in the publication
0.783
quinate
pH 8.8, presence of 2 mM NAD+
10.2
quinate
pH 9.0, 30°C
0.0046
shikimate
pH 8.8, presence of 2 mM NADP+
0.027
shikimate
pH 9.0, 30°C, recombinant isozyme VvSDH3
0.03
shikimate
-
pH 9.0, 25°C, with NADP+
0.0326
shikimate
pH 9.0, temperature not specified in the publication
0.0326
shikimate
pH 9.0, 30°C, EcDQD/SDH1, shikimate oxidation
0.03746
shikimate
-
pH not specified in the publication, 25°C
0.0375
shikimate
-
pH not specified in the publication, 25°C, with NADP+
0.0424
shikimate
25°C, pH 9.0
0.0425
shikimate
with NADP+, pH 9.0, 25°C
0.05
shikimate
pH 7.0, 25°C, recombinant AroE
0.0502
shikimate
-
pH 7.0, 25°C, with NADP+
0.0527
shikimate
-
pH 8.8, temperature not specified in the publication
0.0527
shikimate
pH 8.8, 25°C, with NADP+
0.063
shikimate
-
pH 9.0, 25°C
0.065
shikimate
-
with NADP+, pH 9.0, 20°C
0.07
shikimate
pH 9.0, 30°C, recombinant isozyme VvSDH1
0.073
shikimate
wild-type
0.073
shikimate
pH 7.0, 25°C, with NADP+
0.0786
shikimate
pH 8.8, presence of 2 mM NAD+
0.0786
shikimate
in the presence of 2 mM NAD+
0.101
shikimate
-
pH and temperature not specified in the publication
0.105
shikimate
pH 8.8, presence of 2 mM NADP+
0.105
shikimate
in the presence of 2 mM NADP+
0.12
shikimate
-
pH and temperature not specified in the publication
0.13
shikimate
pH 9.0, 25°C, recombinant wild-type enzyme
0.13
shikimate
22°C, pH 9.0
0.131
shikimate
-
pH 7.5, 22°C, mutant K385N/D423N
0.14
shikimate
pH 9.0, 30°C
0.14
shikimate
with NADP+, pH 9.0, 30°C
0.1468
shikimate
pH 9.0, 30°C, recombinant isozyme VvSDH4
0.148
shikimate
pH 8.0, 60°C, recombinant enzyme
0.148
shikimate
-
with NADP+, pH 8.0, 25°C
0.155
shikimate
pH 7.0, 30°C, recombinant isozyme VvSDH1
0.17
shikimate
-
87°C, pH 7.3
0.17
shikimate
with NADP+, pH 7.3, 87°C
0.178
shikimate
pH 8.8, 25°C, with NADP+
0.187
shikimate
pH 7.0, 30°C
0.19 - 0.28
shikimate
-
-
0.2
shikimate
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHc, with NADP+
0.218
shikimate
-
pH and temperature not specified in the publication
0.22
shikimate
-
2 KM-values
0.223
shikimate
-
pH 8.5, 22°C, with NADP+, isozyme Poptr1
0.223
shikimate
-
with NADP+, pH 8.5, 22°C, recombinant His-tagged Poptr1
0.227
shikimate
mutant Y211F
0.239
shikimate
-
pH and temperature not specified in the publication
0.263
shikimate
-
pH 7.5, 22°C, mutant K385A
0.273
shikimate
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHa, with NADP+
0.279
shikimate
-
pH and temperature not specified in the publication
0.311
shikimate
with NADP+, pH 7.0, 25°C
0.321
shikimate
-
with NADP+, pH 8.5, 22°C, recombinant His-tagged Poptr1
0.346
shikimate
-
pH 8.5, 22°C, with NADP+, isozyme Poptr5
0.346
shikimate
-
with NAD+, pH 8.5, 22°C, recombinant His-tagged Poptr1
0.351
shikimate
pH 8.8, presence of 2 mM NAD+
0.422
shikimate
pH 7.0, 30°C, recombinant isozyme VvSDH3
0.427
shikimate
-
with NAD+, pH 8.5, 22°C, recombinant His-tagged Poptr1
0.459
shikimate
-
mutant T381S, 22°C
0.47
shikimate
-
pH 7.5, 22°C, mutant K385N
0.548
shikimate
pH and temperature not specified in the publication, mutant S338G
0.555
shikimate
-
pH 7.5, 22°C, mutant D423N
0.556
shikimate
-
mutant T381A, 22°C
0.604
shikimate
pH and temperature not specified in the publication, wild-type enzyme
0.611
shikimate
pH 7.5, 30°C, recombinant isozyme CsDQD/SDHD, with NADP+
0.626
shikimate
-
mutant H335A, 22°C
0.685
shikimate
-
pH 7.5, 22°C, wild-type DELTA88DHQ-SDH enzyme variant
0.685
shikimate
-
wild-type, 22°C
0.685
shikimate
pH 8.8, 22°C, with NADP+
0.882
shikimate
pH and temperature not specified in the publication, mutant S338G/T381G
0.91
shikimate
-
2 KM-values
0.92
shikimate
-
mutant Q78L, 22°C
1.022
shikimate
-
mutant T407A, 22°C
1.03
shikimate
-
pH 7.5, 22°C, mutant Y550A
1.06
shikimate
-
pH 7.5, 22°C, mutant Y550F
1.09
shikimate
-
mutant T422S, 22°C
1.539
shikimate
pH and temperature not specified in the publication, mutant T381S
1.613
shikimate
pH and temperature not specified in the publication, mutant T381G
2.05
shikimate
pH 9.0, temperature not specified in the publication
2.05
shikimate
pH 9.0, 30°C, EcDQD/SDH2, shikimate oxidation
2.512
shikimate
pH and temperature not specified in the publication, mutant T381A
3.64
shikimate
pH 9.0, temperature not specified in the publication
3.64
shikimate
pH 9.0, 30°C, EcDQD/SDH3, shikimate oxidation
4.2
shikimate
pH 8.8, presence of 2 mM NAD+
4.466
shikimate
-
mutant Q582L, 22°C
6.2
shikimate
-
pH 7.5, 22°C, mutant S338A
7.12
shikimate
-
mutant N406A, 22°C
8.74
shikimate
-
pH 7.5, 22°C, mutant S336A
46.6
shikimate
pH 10.0, 30°C
0.05
shikimic acid
-
NADP+
0.087
shikimic acid
-
NADP+
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
kinetic profile of the recombinant AroE, overview
-
additional information
additional information
steady-state kinetics of recombinant AroE
-
additional information
additional information
-
steady-state kinetics of recombinant AroE
-
additional information
additional information
no measurable activity with NAD+
-
additional information
additional information
no measurable activity with NAD+
-
additional information
additional information
no measurable activity with NAD+
-
additional information
additional information
no measurable activity with NAD+
-
additional information
additional information
no measurable activity with NAD+
-
additional information
additional information
no measurable activity with NADH+
-
additional information
additional information
no measurable activity with NADH+
-
additional information
additional information
no measurable activity with NADH+
-
additional information
additional information
no measurable activity with NADH+
-
additional information
additional information
no measurable activity with NADH+
-
additional information
additional information
no measurable activity with quinate
-
additional information
additional information
no measurable activity with quinate
-
additional information
additional information
no measurable activity with quinate
-
additional information
additional information
no measurable activity with quinate
-
additional information
additional information
no measurable activity with quinate
-
additional information
additional information
no measurable activity with shikimate
-
additional information
additional information
no measurable activity with shikimate
-
additional information
additional information
no measurable activity with shikimate
-
additional information
additional information
no measurable activity with shikimate
-
additional information
additional information
no measurable activity with shikimate
-
additional information
additional information
steady-state kinetics of wild-type and mutant enzymes, overview
-
additional information
additional information
-
steady-state kinetics of wild-type and mutant enzymes, overview
-
additional information
additional information
-
the enzyme shows Michaelis-Menten kinetics toward both substrates shikimate and NADP+, kinetic analysis, sequential random mechanism, overview
-