Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.1.1.215: gluconate 2-dehydrogenase

This is an abbreviated version!
For detailed information about gluconate 2-dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.215

Reaction

D-gluconate
+
NADP+
=
2-dehydro-D-gluconate
+
NADPH
+
H+

Synonyms

2-HDH, 2-keto-D-gluconate reductase, 2-keto-D-gluconate-yielding D-gluconate dehydrogenase, 2-keto-L-gulonate reductase, 2-ketoaldonate reductase, 2-ketogluconate dehydrogenase, 2-ketogluconate reductase, 2KGA reductase, 2KGR, 2KR, FAD-GADH, GA2DH, GAD, GADH, gluconate dehydrogenase, gluconate-2-dehydrogenase, gluconate-2-DH, GluD, Gox0417, GOX1230, HDH, L-2-hydroxyacid dehydrogenase, NADP-gluconate 2-dehydrogenase, NADPH requiring 2-keto-L-gulonate reductase, reductase, 2-ketogluconate

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.215 gluconate 2-dehydrogenase

Cloned

Cloned on EC 1.1.1.215 - gluconate 2-dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression of wild-type and mutant large subunits
DNA and amino acid sequence determination and analysis, overexpression of wild-type and expression of start codon mutated enzyme in Escherichia coli strain DH5alpha
expressed in Escherichia coli
-
expression in Escherichia coli
expression in Saccharomyces cerevisiae
gene ga2dh, recombinant overexpression of the enzyme in Gluconobacter oxydans strain DSM2003 under the control of three promoters, PtufB, Pga2dh or Pghp0169, respectively. Recombinant strain Gluconobacter oxydans_tufB_ga2dh shows a similar growth rate to that of the control strain and displays the highest specific productivity of 2-dehydro-D-gluconate from D-gluconate, which is increased nearly twofold compared with that of the control strain during batch biotransformation, method optimization
gene gluD, clustered with gene gluE, DNA and amino acid sequence determination and analysis, recombinant expression in Saccharomyces cerevisiae strains ATCC 90845 and modified CEN.PK2, subcloning in Escherichia coli strain TOP10
mutagenized cj0414 and cj0415 alleles are introduced into Campylobacter jejuni 81-176
-
recombinant overexpression of C-terminally His6-tagged enzyme in Escherichia coli
-