1.1.1.201: 7beta-hydroxysteroid dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about 7beta-hydroxysteroid dehydrogenase (NADP+), go to the full flat file.

Reaction

Synonyms

7beta-HSD, 7beta-HSDH, 7beta-hydroxysteroid dehydrogenase, More, NADP-dependent 7beta-hydroxysteroid dehydrogenase, RUMGNA_02585

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.201 7beta-hydroxysteroid dehydrogenase (NADP+)

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Results	in table
15	AA Sequence
5	Application
1	CAS Registry Number
6	Cloned(Commentary)
22	Cofactor
2	Crystallization (Commentary)
4	General Information
2	General Stability
2	Inhibitors
12	kcat/KM [mM/s]
39	KM Value [mM]
5	Localization
7	Molecular Weight [Da]
50	Natural Substrates/ Products (Substrates)
28	Organism
3	Pathway
2	PDB ID
12	pH Optimum
1	pH Range
19	Protein Variants
7	Purification (Commentary)
2	Reaction
3	Reaction Type
26	Reference
12	Source Tissue
6	Specific Activity [micromol/min/mg]
1	Storage Stability
88	Substrates and Products (Substrate)
9	Subunits
32	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
7	Temperature Stability [°C]
17	Turnover Number [1/s]

KM Value

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KM Value on EC 1.1.1.201 - 7beta-hydroxysteroid dehydrogenase (NADP+)

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.07

3,12-diketocholanoic acid

Blautia producta

-

-

286228

0.123

3,7,12-Triketo-5beta-cholanoic acid

Blautia producta

-

-

286228

0.145

3,7,12-triketo-5beta-cholanoyl taurine

Blautia producta

-

-

286228

0.087

3,7-Diketo-5beta-cholanoic acid

Blautia producta

-

-

286228

0.142

3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid

Blautia producta

-

-

286228

0.076

3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid

Hathewaya limosa

-

-

286227

0.974

3alpha,7beta-dihydroxy-5beta-cholan-24-oic acid

[Ruminococcus] gnavus

A7B4V1

pH 10.0, 37°C, recombinant enzyme

740835

0.009 - 0.108

3alpha,7beta-dihydroxy-5beta-cholanoic acid

4 entries

0.238 - 0.909

3alpha,7beta-dihydroxy-5beta-cholanoyl glycine

2 entries

0.286 - 1.639

3alpha,7beta-dihydroxy-5beta-cholanoyl taurine

2 entries

0.124

3alpha-Hydroxy-7,12-diketo-5beta-cholanoic acid

Blautia producta

-

-

286228

0.055

3alpha-hydroxy-7-keto-5beta-cholanoic acid

Blautia producta

-

-

286228

0.0388

3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid

[Ruminococcus] gnavus

A7B4V1

pH 6.0, 37°C, recombinant enzyme

740835

0.0052

7-ketolithocholic acid

Collinsella aerofaciens

-

pH 8.0, 25°C

724054

0.021 - 0.19

7-oxo-lithocholic acid

10 entries

0.0092

Dehydrocholic acid

Collinsella aerofaciens

-

pH 8.0, 25°C

724054

0.0053 - 0.4

NADP+

5 entries

0.0045 - 0.047

NADPH

3 entries

0.0062

Ursodeoxycholic acid

Collinsella aerofaciens

-

pH 8.0, 25°C

724054

0.009

3alpha,7beta-dihydroxy-5beta-cholanoic acid

Clostridium sardiniense

-

-

286076

0.018

3alpha,7beta-dihydroxy-5beta-cholanoic acid

Hathewaya limosa

-

-

286227

0.022

3alpha,7beta-dihydroxy-5beta-cholanoic acid

Blautia producta

-

-

286226

0.108

3alpha,7beta-dihydroxy-5beta-cholanoic acid

Collinsella aerofaciens

-

-

286226

0.238

3alpha,7beta-dihydroxy-5beta-cholanoyl glycine

Blautia producta

-

-

286226

0.909

3alpha,7beta-dihydroxy-5beta-cholanoyl glycine

Collinsella aerofaciens

-

-

286226

0.286

3alpha,7beta-dihydroxy-5beta-cholanoyl taurine

Blautia producta

-

-

286226

1.639

3alpha,7beta-dihydroxy-5beta-cholanoyl taurine

Collinsella aerofaciens

-

-

286226

0.021

7-oxo-lithocholic acid

[Ruminococcus] torques

-

mutant enzyme I28V/V38A/I76V/V207M, at pH 8.0 and 30°C

761334

0.023

7-oxo-lithocholic acid

[Ruminococcus] torques

-

wild type enzyme, at pH 8.0 and 30°C

761334

0.023

7-oxo-lithocholic acid

[Ruminococcus] torques

-

mutant enzyme N240I, at pH 8.0 and 30°C

761334

0.028

7-oxo-lithocholic acid

[Ruminococcus] torques

-

mutant enzyme I112V/N240D, at pH 8.0 and 30°C

761334

0.031

7-oxo-lithocholic acid

[Ruminococcus] torques

-

mutant enzyme T189V, at pH 8.0 and 30°C

761334

0.034

7-oxo-lithocholic acid

[Ruminococcus] torques

-

mutant enzyme G57S/I163V/V207M, at pH 8.0 and 30°C

761334

0.035

7-oxo-lithocholic acid

[Ruminococcus] torques

-

mutant enzyme V207M, at pH 8.0 and 30°C

761334

0.04

7-oxo-lithocholic acid

[Ruminococcus] torques

-

mutant enzyme T189V/V207M, at pH 8.0 and 30°C

761334

0.057

7-oxo-lithocholic acid

[Ruminococcus] torques

-

mutant enzyme K44E/T189I, at pH 8.0 and 30°C

761334

0.19

7-oxo-lithocholic acid

[Ruminococcus] torques

-

mutant enzyme I112V/T189I/N240D, at pH 8.0 and 30°C

761334

0.0053

NADP+

Collinsella aerofaciens

-

pH 8.0, 25°C

724054

0.072

NADP+

Clostridium sardiniense

-

-

286076

0.1

NADP+

Blautia producta

-

-

286226

0.14

NADP+

Hathewaya limosa

-

-

286227

0.4

NADP+

Collinsella aerofaciens

-

-

286226

0.0045

NADPH

Collinsella aerofaciens

-

pH 8.0, 25°C

724054

0.0078

NADPH

[Ruminococcus] torques

-

wild type enzyme, at pH 8.0 and 30°C

761334

0.047

NADPH

[Ruminococcus] torques

-

mutant enzyme T189V/V207M, at pH 8.0 and 30°C

761334