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1.1.1.195: cinnamyl-alcohol dehydrogenase

This is an abbreviated version!
For detailed information about cinnamyl-alcohol dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.195

Reaction

cinnamyl alcohol
+
NADP+
=
cinnamaldehyde
 +
NADPH
 +
H+

Synonyms

ADH, AdhA, alcohol dehydrogenase, AtCAD4, BdCAD1, Bmr6, Bradi3g06480, brown midrib6, Brown-midrib 1 protein, CAD, CAD 7/8, CAD1, CAD10, CAD11, CAD12, CAD13, CAD14, CAD15, CAD2, CAD3, CAD4, CAD5, CAD6, CAD7, CAD8, CAD9, CADH I, cinnamyl alcohol dehydrogenase, cinnamyl alcohol dehydrogenase 1, cinnamyl alcohol dehydrogenase 12, cinnamyl alcohol dehydrogenase 2, cinnamyl alcohol dehydrogenase 3, cinnamyl alcohol dehydrogenase 4, cinnamyl alcohol dehydrogenase 5, cinnamyl alcohol dehydrogenase 7, cinnamyl alcohol dehydrogenase 9, cinnamyl alcohol dehydrogenase C, cinnamyl alcohol dehydrogenases, CtCAD1, CtCAD2, CtCAD3, dehydrogenase, cinnamyl alcohol, FC1, FLEXIBLE CULM1, HcCAD1, HcCAD2, LlCAD2, LtuCAD1, More, Mt-CAD1, Mt-CAD2, PhCAD1, PhCAD2, PhCAD3, PhCAD4, PtoCAD1, PtoCAD12, PtoCAD2, PtoCAD3, PtoCAD5, PtoCAD6, PtoCAD7, PtoCAD8, PtoCAD9, ScAdh6p, TaCAD12

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.195 cinnamyl-alcohol dehydrogenase

Purification

Purification on EC 1.1.1.195 - cinnamyl-alcohol dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by affinity purification on a nickel column
native enzyme 514.2fold from secondary xylem by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, and affinity chromatography (elution with NADP+)
-
Ni-NTA agarose bead chromatography
Ni-NTA agarose column chromatography and Mono Q column chromatography
Ni-NTA column chromatography
nickel affinity column chromatography
recombinant enzyme
recombinant enzyme from strain BL9 by DEAE ion exchange, Red-Sepharose, and gel filtration chromatography
-
recombinant GH2-encoded CAD from Escherichia coli
-
recombinant GST-fusion enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography
recombinant His-tagged enzyme 65fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, anion exchange chromatography, and again gel filtration
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chrmatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged PtoCAD1 from Escherichia coli strain M15 by nickel affinity chromatography
recombinant His-tagged PtoCAD2 from Escherichia coli strain M15 by nickel affinity chromatography
recombinant His-tagged PtoCAD3 from Escherichia coli strain M15 by nickel affinity chromatography
recombinant His-tagged PtoCAD5 from Escherichia coli strain M15 by nickel affinity chromatography
recombinant His-tagged PtoCAD7 from Escherichia coli strain M15 by nickel affinity chromatography
recombinant His-tagged PtoCAD9 from Escherichia coli strain M15 by nickel affinity chromatography
recombinant His-tagged wild-type and mutant Mt-CAD2 enzymes by nickel affinity chromatography, tag cleavage by thrombin, benzamidine affinity chromatography to remove thrombin, and gel filtration
-
recombinant His-tagged wild-type Mt-CAD1 enzyme by nickel affinity chromatography, tag cleavage by thrombin, benzamidine affinity chromatography to remove thrombin, and gel filtration
-
recombinant isozyme CAD4 from Escherichia coli
recombinant isozyme CAD5 from Escherichia coli
two isoforms
-
var. Mandarin
-