69% of wild-type activity in presence of NADPH, 138% of wild-type isomerization activity in absence of NADPH, 0.2% of wild-type activity reducing 4-nitrobenzaldehyde
mutagenesis to the corresponding residue in other mammalian AKR1C subfamily enzymes, results in broad substrate specificity for nonsteroidal carbonyls and alcohols
lentiviral vector-mediated delivery of the prostaglandin F synthase (PGFS) gene to the eye. Expression of myc-tagged GFP-PGFS in non-human primate eyes via FIV vector leads to a significant reduction of intraocular pressure, IOP, the GFP-tag can still be detected after 48-52 weeks, the decrease in IOP lasts for 5 months. The IOP decrease is not as much as seen with topical prostaglandin therapy in monkey eyes, method evaluation. In human eye a single injection of lentiviral vectors into either the anterior or posterior chambers can induce a transient uveitis. Following transcorneal injection of vector into the living monkey eyes, biomicroscopic examination initially reveal cells and flare in the anterior chamber and some corneal haze that is largely resolved by 3 weeks post injection. This is not due to bacterial endotoxin contamination of the vector preparations and is likely the result of activation of innate viral sensors in the eye
knockout of AKR1B1 gene expression in human endometrial cell lines using the CRISPR/Cas9 system. Clone 16-2 exhibits deletion of 68 and 2 nucleotides, respectively, on each of the alleles. Cells from this clone lose their ability to produce PGF2alpha but maintain their original human endometrial stromal cells-2 phenotype including the capacity to decidualize in the presence of progesterone (medroxyprogesterone acetate) and 8-bromo-cAMP. Knockout cells also maintain their ability to increase PGE2 production in response to interleukin-1beta
knockout of AKR1B1 gene expression in human endometrial cell lines using the CRISPR/Cas9 system. Clone 16-2 exhibits deletion of 68 and 2 nucleotides, respectively, on each of the alleles. Cells from this clone lose their ability to produce PGF2alpha but maintain their original human endometrial stromal cells-2 phenotype including the capacity to decidualize in the presence of progesterone (medroxyprogesterone acetate) and 8-bromo-cAMP. Knockout cells also maintain their ability to increase PGE2 production in response to interleukin-1beta
1.1.1.188 prostaglandin-F synthase
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