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1.1.1.184: carbonyl reductase (NADPH)

This is an abbreviated version!
For detailed information about carbonyl reductase (NADPH), go to the full flat file.

Word Map on EC 1.1.1.184

Reaction

R-CHOH-R'
+
NADP+
=
R-CO-R'
+
NADPH
+
H+

Synonyms

(R) specific carbonyl reductase, (S)-specific carbonyl reductase, 15-hydroxyprostaglandin dehydrogenase [NADP+], 2,5-diketo-D-gluconic acid reductase, Adipocyte P27 protein, aldehyde reductase 1, aldehyde reductase I, aldo-keto reductase, ALR3, AP27, carbonyl reductase, carbonyl reductase (NADPH), carbonyl reductase 1, carbonyl reductase 3, carbonyl reductase S1, CBR, CBR 1, CBR 3, CBR1, CBR3, CHCR, CHCR1, CHCR2, CHCR3, CR, CR125, crc1, CSCR1, Gox0644, Gox1615, hCBR1, ketoreductase, KLCR1, KR, LCR, LOC415661, LOC610164, microsomal carbonyl reductase, More, NADP+-dependent ADH, NADPH-carbonyl reductase, NADPH-dependent carbonyl reductase, NADPH-dependent carbonyl reductase S1, NCCR, nonspecific NADPH-dependent carbonyl reductase, peroxisomal-type carbonyl reductase, PHCR, prostaglandin 9-ketoreductase, Prostaglandin-E2 9-reductase, PTCR, R-specific carbonyl reductase, reductase S1, reductase, carbonyl, RLCR, SCR, SCR9, SDR21C1, secondary-alcohol: NADP+-oxidoreductase, short-chain (S)-1-phenyl-1,2-ethanediol dehydrogenase, short-chain carbonyl reductase, sniffer, SRED, SSCR, SyS1, tetrameric carbonyl reductase, Tm1743, Tm_1743, xenobiotic carbonyl reductase, xenobiotic ketone reductase, YGL039w1, YGL039w2, YtbE, YueD

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.184 carbonyl reductase (NADPH)

Temperature Stability

Temperature Stability on EC 1.1.1.184 - carbonyl reductase (NADPH)

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 55
-
the enzyme has half-lives of 18.57, 12.99, 0.76 and 0.06 h at 25, 35, 45 and 55°C respectively
30 - 60
purified recombinant enzyme, stable for 15 h
35
stable for 60 min
35 - 55
-
about 60% of enzymatic activity is maintained after 30 min of incubation at 35°C. However, the activity is completely lost after incubation at 55°C for 30 min
40 - 50
-
when incubated at 40°C and 50°C for 2 h, the enzyme maintains residual enzyme activity of more than 70%
48
melting temperature, mutant S172T and N-terminal deletion mutant lacking 31 amino acids
52
melting temperature, wild-type
60
-
rapid inactivation above
65
Candida macedoniensis
-
pH 7.0, 0.1 mM DTT, complete loss of activity after 10 min
90
10 min, 75% residual activity
additional information
-
activation by arachidonic acid leads to a decrease in thermal stability