1.1.1.103: L-threonine 3-dehydrogenase
This is an abbreviated version!
For detailed information about L-threonine 3-dehydrogenase, go to the full flat file.
Reaction
Synonyms
CLOST_1621, L-ThrDH, L-threonine dehydrogenase, More, orf382, TDG, TDH, Thr dehydrogenase, ThrDH, threonine 3-dehydrogenase, threonine dehydrogenase
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Cloned
Cloned on EC 1.1.1.103 - L-threonine 3-dehydrogenase
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DNA and amino acid seuence determination and analysis, sequence comparisons, phylogenetic tree, expression of His-tagged enzyme in Escherichia coli strains JM109 and BL21(DE3)
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
into pET-30a digested with KpnIEcoRI to construct pET-ste11 and expressed in Escherichia coli BL21
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ligated into the pTZ57R/T cloning vector. The recombinant pTZ-tdh plasmid digested with NcoI and EcoRI and the TDH gene ligated with pET-8c, giving the recombinant pET-tdh plasmid, used to transform Escherichia coli DH5alpha competent cells. Recombinant and purified pET-tdh plasmid expressed in Escherichia coli BL21 (DE3)
orf PH0655, amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain TOP10
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orf PH0655, gene TDH, overexpression in Escherichia coli strain BL21(DE3)
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orf PH0655, NA and amino acid sequence determination, analysis, and comparison, expression of selenomethionine-labeled wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
orf PH0655, overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
plasmid pTZ-tdh transformed in Escherichia coli DH5alpha competent cells. NcoI-EcoRI restriction fragment inserted into the pET-8c expression vector at the corresponding sites. The resulting plasmid pET-tdh expressed in Escherichia coli strain BL21(DE3)CodonPlus-RIL
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recombinant pET-tdh plasmid expressed in Escherichia coli BL21 (DE3)