1.1.1.1: alcohol dehydrogenase

1% activity compared to cyclohexanone

(1S,3S)-3-methylcyclohexanol + NAD+

(rac)-3-methylcyclohexanone + NADH + H+

125% activity compared to cyclohexanol

163% activity compared to cyclohexanone

(2E)-2-methylpent-2-enal + NADPH + H+

(2E)-2-methylpent-2-en-1-ol + NADP+

41.5% of the activity with (2E)-but-2-enal, yield 95% after 12 h

(2E)-3,7-dimethylocta-2,6-dienal + NADPH + H+

(2E)-3,7-dimethylocta-2,6-dien-1-ol + NADP+

37.4% of the activity with (2E)-but-2-enal, yield 87% after 12 h

(2E)-4-hydroxynon-2-enal + NADH + H+

(2E)-non-2-ene-1,4-diol + NAD+

substrate of isozyme ADH4

691867

(2E)-but-2-en-1-ol + NADP+

(2E)-but-2-enal + NADPH + H+

(2E)-but-2-enal + NADPH + H+

(2E)-but-2-en-1-ol + NADP+

yield 96% after 12 h

(2E)-dec-2-enal + NADPH + H+

(2E)-dec-2-en-1-ol + NADP+

16.7% of the activity with (2E)-but-2-enal, yield 91% after 12 h

(2E)-hex-2-enal + NADPH + H+

(2E)-hex-2-en-1-ol + NADP+

41.7% of the activity with (2E)-but-2-enal, yield 98% after 12 h

(2E)-oct-2-enal + NADPH + H+

(2E)-oct-2-en-1-ol + NADP+

30.2% of the activity with (2E)-but-2-enal, yield 69% after 12 h

plus 27% oct-2-enyl ester

(6S)-5,6-dihydro-6-methyl-4H-thieno[2,3b]thiopyran-4-one-7,7-dioxide + NADH + H+

(4S,6S)-5,6-dihydro-4-hydroxy-6-methyl-4H-thieno[2,3b]thiopyran-7,7-dioxide + NAD+

Neurospora crassa

659773

(E)-hex-2-en-1-ol + NAD+

(E)-hex-2-en-1-one + NADH

(R)-1-indanol + NAD+

1-indanone + NADH + H+

(R)-1-indanol + NAD+

62% of activity compared to (S)-1-indanol

(R)-1-phenyl-1-butanol + NAD+

1-phenylbutan-1-one + NADH + H+

(R)-1-phenyl-1-propanol + NAD+

1-phenyl-1-propanone + NADH + H+

(R)-1-phenyl-2-butanol + NAD+

1-phenylbutan-2-one + NADH + H+

(R)-1-phenylethanol + NAD+

acetophenone + NADH + H+

(R)-2-butanol + NAD+

2-butanone + NADH + H+

(R)-2-butanol + NAD+

butanone + NADH + H+

285586

(R)-2-heptanol + NAD+

2-heptanone + NADH + H+

(R)-2-hexanol + NAD+

2-hexanone + NADH + H+

(R)-2-octanol + NAD+

2-octanone + NADH + H+

(R)-2-pentanol + NAD+

2-pentanone + NADH + H+

(R)-2-phenylpropanol + NAD+

(R)-2-phenylpropanal + NADH + H+

(R)-3-methylcyclohexanone + NADH + H+

? + NAD+

2% activity compared to cyclohexanone

(R)-4-phenyl-2-butanol + NAD+

4-phenylbutan-2-one + NADH + H+

(R)-alpha-tetralol + NAD+

58% of activity compared to (S)-1-indanol

(R)-alpha-tetralol + NAD+

alpha-tetralone + NADH + H+

(R,S)-2-methylbutan-1-ol + NAD+

(R,S)-2-methyl-butan-1-one + NADH + H+

(S)-(+)-1-indanol + NAD+

indanone + NADH + H+

(S)-1-indanol + NAD+

1-indanone + NADH + H+

(S)-1-indanol + NAD+

(S)-1-phenyl-1-butanol + NAD+

1-phenylbutan-1-one + NADH + H+

no substrate of mutant B1

(S)-1-phenyl-1-propanol + NAD+

1-phenyl-1-propanone + NADH + H+

(S)-1-phenyl-2-butanol + NAD+

1-phenylbutan-2-one + NADH + H+

(S)-1-phenyl-2-propanol + NAD+

phenylacetone + NADH + H+

9% activity compared to cyclohexanone

(S)-1-phenylethanol + NAD+

1-phenylethanone + NADH + H+

(S)-1-phenylethanol + NAD+

acetophenone + NADH + H+

(S)-2-butanol + NAD+

2-butanone + NADH

(S)-2-butanol + NAD+

2-butanone + NADH + H+

(S)-2-butanol + NAD+

butanone + NADH + H+

(S)-2-heptanol + NAD+

2-heptanone + NADH + H+

331% of the activity with 2-propanol

(S)-2-hexanol + NAD+

2-hexanone + NADH + H+

38% of the activity with 2-propanol

(S)-2-methylbutan-1-ol + NAD+

(S)-2-methyl-butanal + NADH + H+

(S)-2-octanol + NAD+

2-octanone + NADH + H+

(S)-2-pentanol + NAD+

2-pentanone + NADH + H+

(S)-2-pentanol + NAD+

11% of activity compared to (S)-1-indanol

(S)-2-phenylpropanol + NAD+

(S)-2-phenylpropanal + NADH + H+

156% of the activity with 2-phenylethanol

(S)-4-phenyl-2-butanol + NAD+

4-phenylbutan-2-one + NADH + H+

(S)-4-phenylbutan-2-ol + NAD+

benzylacetone + NADH + H+

3% activity compared to cyclohexanone

(S)-alpha-tetralol + NAD+

12% of activity compared to (S)-1-indanol

(S)-alpha-tetralol + NAD+

alpha-tetralone + NADH + H+

(S)-heptan-2-ol + NAD+

2-heptanone + NADH + H+

(S)-pentan-2-ol + NAD+

2-pentanone + NADH + H+

(S)-perillylalcohol + NAD+

(S)-perillaldehyde + NADH + H+

52% activity compared to cyclohexanol

(Z)-hex-2-en-1-ol + NAD+

(Z)-hex-2-en-1-one + NADH

Camellia sinensis

9.7% of the activity with ethanol

285628

1,1-dichloroacetone + NADH + H+

1,1-dichloropropan-2-ol + NAD+

1078% of the activity with phenyl trifluoromethyl ketone

1,2-butanediol + NAD+

1,2-butanediol + NAD+

? + NADH + H+

1,2-hexanediol + NAD+

20% of the activity with (S)-N-benzyl-3-pyrrolidinol

1,2-pentanediol + NAD+

12% of the activity with (S)-N-benzyl-3-pyrrolidinol

1,2-propanediol + NAD+

1,2-propanediol + NAD+

hydroxyacetone + NADH

Fragaria x ananassa

669027

1,2-propanediol + NAD+

hydroxyacetone + NADH + H+

Methylorubrum extorquens

669027

1,3-butanediol + NAD+

39% of the activity with (S)-N-benzyl-3-pyrrolidinol

1,3-propanediol + 2 NAD+ + H2O

propanedial + 2 NADH + 2 H+

1,3-propanediol + NAD+

1,3-propanediol + NAD+

? + NADH

Megalodesulfovibrio gigas

7% of the activity with ethanol

285654

1,3-propanediol + NAD+

? + NADH + H+

1,4-butanediol + NAD+

? + NADH + H+

best substrate in oxidation reaction

1,5-pentanediol + NAD+

? + NADH + H+

Mycobacterium sp.

W8VSK8

18% of the activity with butan-1-ol

737865

1-(3-bromophenyl)ethanol + NAD+

1-(3-bromophenyl)ethanone + NADH + H+

315% of the activity with 1-phenylethanol

1-(3-chlorophenyl)ethanol + NAD+

1-(3-chlorophenyl)ethanone + NADH + H+

205% of the activity with 1-phenylethanol

1-(4'-chlorophenyl)ethanol + NAD+

1-(4'-chlorophenyl)ethanone + NADH + H+

26% of the activity with (S)-(-)-1-phenylethanol

1-(4'-fluorophenyl)ethanol + NAD+

1-(4'-fluorophenyl)ethanone + NADH + H+

45% of the activity with (S)-1-phenylethanol

1-(4-bromophenyl)ethanol + NAD+

1-(4-bromophenyl)ethanone + NADH + H+

167% of the activity with 1-phenylethanol

1-(4-chlorophenyl)ethanol + NAD+

1-(4-chlorophenyl)ethanone + NADH + H+

151% of the activity with 1-phenylethanol

1-(4-fluorophenyl)ethanol + NAD+

1-(4-fluorophenyl)ethanone + NADH + H+

1-(4-methylphenyl)ethanol + NAD+

1-(4-methylphenyl)ethanone + NADH + H+

189% of the activity with 1-phenylethanol

1-(p-tolyl)-ethanol + NAD+

1-(4-methylphenyl)ethanone + NADH + H+

19% activity compared to cyclohexanol

26% activity compared to cyclohexanone

1-butanal + NADH + H+

1-butanol + NAD+

1-butanol + NAD+

butanal + NADH

1-butanol + NAD+

butanal + NADH + H+

25 entries

1-butanol + NAD+

butyraldehyde + NADH + H+

1-chloro-5-acetylfuro[2,3-c]pyridine + NADH + H+

1-chloro-5-(1-hydroxyethyl)furo[2,3-c]pyridine + NAD+

1-decalone + NADH + H+

decahydronaphthalen-1-ol + NAD+

85% of the activity compared to 1-phenyl-1,2-propanedione

1-decanol + NAD+

decanal + NADH + H+

1-dodecanol + NAD+

dodecanal + NADH

1-heptanol + NAD+

1-heptanal + NADH + H+

1-heptanol + NAD+

heptanal + NADH + H+

9 entries

1-hexanal + NADH + H+

1-hexanol + NAD+

1-hexanol + NAD+

1-hexanal + NADH + H+

1-hexanol + NAD+

15% of the activity with (S)-N-benzyl-3-pyrrolidinol

1-hexanol + NAD+

hexanal + NADH + H+

15 entries

1-hydroxy-2-butanone + NADH + H+

butane-1,2-diol + NAD+

59% of the activity with N-benzyl-3-pyrrolidinone

1-hydroxymethyl-6-methylpyrene + NAD+

1-formyl-6-methylpyrene + NADH + H+

1-hydroxymethyl-8-methylpyrene + NAD+

1-formyl-8-methylpyrene + NADH + H+

1-hydroxymethylpyrene + NAD+

1-formylpyrene + NADH + H+

1-indanol + NAD+

1-indanone + NADH + H+

1-indanone + NADH + H+

(S)-1-indanol + NAD+

1-indanone + NADH + H+

1-indanol + NAD+

1-nonanol + NAD+

nonanal + NADH + H+

1-octanol + NAD+

1-octanal + NADH + H+

6% of activity with N-benzyl-3-pyrrolidinol

1-octanol + NAD+

octanal + NADH

1-octanol + NAD+

octanal + NADH + H+

1-pentanol + NAD+

1-pentanal + NADH + H+

1-pentanol + NAD+

pentanal + NADH + H+

9 entries

1-phenyl-1,2-ethanediol + NAD+

1-phenyl-2-propanone + NADH + H+

1% activity compared to cyclohexanol

1-phenyl-1,2-propandione + NADH + H+

1-phenyl-2-hydroxy-1-propanone + NAD+

146% of the activity with 2,2,2-trifluoroacetophenone

1-phenyl-1,2-propanedione + NADH

about 25% of the activity compared to isatin

1-phenyl-1,2-propanedione + NADH + H+

1-phenyl-1-propanol + NAD+

1-phenyl-1-propanone + NADH + H+

59% of the activity with (S)-(-)-1-phenylethanol

1-phenyl-1-propanol + NAD+

1-phenylpropan-1-one + NADH + H+

59% of the activity with (S)-1-phenylethanol

1-phenyl-1-propanol + NAD+

31% of the activity with (S)-N-benzyl-3-pyrrolidinol

1-phenyl-2-butanone + NADH + H+

1-phenylbutan-2-ol + NAD+

1-phenyl-2-propanol + NAD+

1-phenyl-2-propanone + NADH + H+

1-phenyl-2-propanone + NADH + H+

(S)-1-phenyl-2-propanol + NAD+

Rhodococcus erythropolis

658321

1-phenyl-3-butanone + NADH + H+

1-phenylbutan-3-ol + NAD+

353% of the activity with phenyl trifluoromethyl ketone

1-phenylethanol + NAD+

1-phenylethanone + NADH

1-phenylethanol + NAD+

1-phenylethanone + NADH + H+

1-phenylethanol + NAD+

acetophenone + NADH + H+

1% activity compared to cyclohexanone

1-phenylethanol + NADP+

1-phenylethanone + NADPH + H+

1-propanol + NAD+

propanal + NADH

Fragaria x ananassa

669027

1-propanol + NAD+

propanal + NADH + H+

1-propanol + NAD+

propanaldehyde + NADH + H+

1-propanol + NAD+

propionaldehyde + NADH + H+

762802

11-cis-retinal + NADH + H+

11-cis-retinol + NAD+

285584

11-cis-retinol + NAD+

11-cis-retinal + NADH

12-hydroxydodecanoate + NAD+

12-oxododecanoic acid + NADH

12-hydroxylauric acid methyl ester + NAD+

12-oxolauric acid methyl ester + NADH + H+

product is a key intermediate for biobased polyamide 12 production

12-oxolauric acid methyl ester + NADH + H+

12-hydroxylauric acid methyl ester + NAD+

13-cis-retinal + NADH + H+

13-cis-retinol + NAD+

285584

13-cis-retinol + NAD+

13-cis-retinal + NADH

16-hydroxyhexadecanoate + NAD+

16-oxohexadecanoic acid + NADH

285568, 285569

17beta-hydroxyetiocholan-3-one + NAD+

ethiocholan-3,17-dione + NADH

285571

2',3',4',5',6'-pentafluoroacetophenone + NADH + H+

1-(2,3,4,5,6-pentafluorophenyl)ethanol + NAD+

45% of the activity compared to 1-phenyl-1,2-propanedione

2,2'-dichlorobenzil + NADH + H+

1,2-bis(2-chlorophenyl)-2-hydroxyethanone + NAD+

2,2,2-trichloroethanol + NAD+

trichloroacetaldehyde + NADH + H+

Thermomicrobium roseum

655262

2,2,2-trifluoro-1-phenylethanone + propan-2-ol

(1R)-2,2,2-trifluoro-1-phenylethanol + acetone

in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH

99% conversion, 99% enantiomeric excess

2,2,2-trifluoroacetophenone + NADH

2,2,2-trifluoro-1-phenylethanol + NAD+

about 35% of the activity compared to isatin

2,2,2-trifluoroacetophenone + NADH + H+

(R)-alpha-(trifluoromethyl)benzyl alcohol + NAD+

2,2,2-trifluoroacetophenone + NADH + H+

2,2,2-trifluoro-1-phenylethanol + NAD+

2,2,2-trifluoroacetophenone + NADPH + H+

(R)-2,2,2-trifluoro-1-phenylethanol + NADP+

180% of the activity with acetoin

2,2,2-trifluoroethanol + NAD+

2,2,2-trifluoroethanone + NADH + H+

760441

2,2-dichloroacetophenone + NADH + H+

2,2-dichloro-1-phenylethanol + NAD+

2,2-difluoro-1-phenylethanone + propan-2-ol

(1R)-2,2-difluoro-1-phenylethanol + acetone

in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH

99% conversion, 94% enantiomeric excess

2,3'-dichloroacetophenone + NADH + H+

2-chloro-1-(3-chlorophenyl)ethanol + NAD+

2,3,4,5,6-pentafluorobenzyl alcohol + NAD+

2,3,4,5,6-pentafluorobenzaldehyde + NADH + H+

2,3-butanediol + H2O

2,3-butanediol + NAD+

3-hydroxy-2-butanone + NADH + H+

2,3-butanediol + NAD+

83% of the activity with (S)-N-benzyl-3-pyrrolidinol

2,3-butanediol + NAD+

acetoin + NADH + H+

2,3-butanediol + NADP+

3-hydroxy-2-butanone + NADPH + H+

2,3-butanediol + NADP+

acetoin + NADPH + H+

2,3-butanedione + NADH + H+

activity is 1.6fold higher than with N-benzyl-3-pyrrolidinone

2,3-pentanedione + NADH + H+

? + NAD+

Petroselinum crispum

5.5% of the activity with acetaldehyde

285627

2,4-pentanediol + NAD+

2-(3,5-dimethylphenyl)propanal + NADH + H+

(S)-2-(3,5-dimethylphenyl)propanol + NAD+

2-(3-benzoylphenyl)propanal + NADH + H+

(S)-2-(3-benzoylphenyl)propanol + NAD+

2-(3-fluorobiphenyl-4-yl)propanal + NADH + H+

(S)-2-(3-(fluoro)biphenyl-4-yl)propanol + NAD+

2-(3-phenoxyphenyl)propanal + NADH + H+

(S)-2-((3-phenoxy)phenyl)propanol + NAD+

2-(4-isobutylphenyl)propanal + NADH + H+

(S)-2-(4-isobutylphenyl)propanol + NAD+

2-(4-trifluoromethylphenyl)propanal + NADH + H+

(S)-2-(4-trifluoromethyl)phenylpropanol + NAD+

18 h, 55% yield, 98% enantiomeric excess

2-(6-methoxynaphthalen-2-yl)propanal + NADH + H+

(S)-2-(6-methoxynaphthalen-2-yl)propan-1-ol + NAD+

18 h, 96% yield, 98% enantiomeric excess

2-(naphthalen-1-yl)propanal + NADH + H+

(S)-2-(naphthalen-1-yl)propanol + NAD+

18 h, 90% yield, 80% enantiomeric excess

2-(naphthalen-2-yl)propanal + NADH + H+

(S)-2-(naphthalen-2-yl)propanol + NAD+

18 h, 57% yield, 94% enantiomeric excess

2-acetylcyclohexanone + NADH + H+

2-acetylcyclohexanol + NAD+

2-acetylcyclopentanone + NADH + H+

2-acetylcyclopentanol + NAD+

2-acetylfuran + NADH + H+

? + NAD+

2-acetylpyridine + NADH + H+

(R)-1-(2-pyridyl)ethanol + NAD+

2-acetylpyrrole + NADH + H+

1-(1H-pyrrol-2-yl)ethanol + NAD+

70% of activity with N-benzyl-3-pyrrolidinone

2-acetylpyrrole + NADH + H+

? + NAD+

2-acetylthiazole + NADH + H+

? + NAD+

2-acetylthiophene + NADH + H+

? + NAD+

2-aminoethanol + NAD+

Picrophilus torridus

Q6L0S1

684620

2-butanol + NAD+

2-butanone + NADH + H+

2-butanone + NADH + H+

2-butanol + NAD+

2-butanone + NADH + H+

butan-2-ol + NAD+

2.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone

2-butanone + NADPH + H+

butan-2-ol + NADP+

12.1% of the activity with 2-hydroxyacetophenone and NADPH

2-butene-1-ol + NAD+

? + NADH

2-chloro-1-(2,4-dichlorophenyl)ethan-1-one + NADH + H+

(1R)-2-chloro-1-(2,4-dichlorophenyl)ethan-1-ol + NAD+

AE010289.1

production of with (1R)-2-chloro-1-(2,4-dichlorophenyl)ethan-1-ol 99% enantiomeric excess

2-chloro-1-(2,4-difluorophenyl)ethan-1-one + NADH + H+

(1R)-2-chloro-1-(2,4-fluorophenyl)ethan-1-ol + NAD+

AE010289.1

production of (1R)-2-chloro-1-(2,4-fluorophenyl)ethan-1-ol with 99% enantiomeric excess

2-chloro-1-(4-fluorophenyl)ethan-1-one + NADH + H+

(1R)-2-chloro-1-(4-fluorophenyl)ethan-1-ol + NAD+

AE010289.1

production of (1R)-2-chloro-1-(4-fluorophenyl)ethan-1-ol with 98% enantiomeric excess

2-chloroacetophenone + NADH + H+

1-(2-chlorophenyl)ethanol + NAD+

low activity

2-chlorocyclohexanone + NADH + H+

? + NAD+

3% activity compared to cyclohexanone

2-decalone + NADH + H+

? + NAD+

28% activity compared to cyclohexanone

Flavobacterium frigidimaris

2-decanol + NAD+

2-decanone + NADH + H+

Q8L3C9

weak activity

668532

2-decanone + NADH

(S)-2-decanol + NAD+ + H+

92% enantiomeric excess

2-dehydro-3-deoxy-D-gluconate + NADH + H+

4-deoxy-L-erythro-5-hexoseulose + NAD+

2-deoxy-D-ribose + NAD+

? + NADH

285569

2-ethoxyethanol + NAD+

2-ethoxyacetaldehyde + NADH + H+

2-ethylhexan-1-ol + NAD+

2-ethylhexanal + NADH

Sporotrichum pulverulentum

weak activity

285639

2-fluoro-1-(4-chlorophenyl)ethanone + propan-2-ol

(1R)-2-fluoro-1-(4-chlorophenyl)ethanol + acetone

in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH

99% conversion, 98% enantiomeric excess

2-fluoro-1-(4-fluorophenyl)ethanone + propan-2-ol

(1R)-2-fluoro-1-(4-fluorophenyl)ethanol + acetone

in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH

99% conversion, 97% enantiomeric excess

2-fluoro-1-(4-iodophenyl)ethanone + propan-2-ol

(1R)-2-fluoro-1-(4-iodophenyl)ethanol + acetone

in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH

99% conversion, 99% enantiomeric excess

2-fluoro-1-phenylethanone + propan-2-ol

(1R)-2-fluoro-1-phenylethanol + acetone

in presence of propan-2-ol at 10% v/v, reduction of fluorinated ketones is catalyzed without addition of NADH

99% conversion, 99% enantiomeric excess

2-heptanol + NAD+

2-heptanone + NADH + H+

2-heptanol + NAD+

heptan-2-one + NADH + H+

62% of the activity with 1-phenylethanol

2-heptanone + NADH

(S)-2-heptanol + NAD+ + H+

79% enantiomeric excess

2-heptanone + NADH + H+

(R)-2-heptanol + NAD+

229% of the activity with phenyl trifluoromethyl ketone

99% enantiomeric excess

2-heptanone + NADPH + H+

heptan-2-ol + NADP+

6.8% of the activity with 2-hydroxyacetophenone and NADPH

2-hexanol + NAD+

2-hexanone + NADH + H+

2-hexanone + NADH

(S)-2-hexanol + NAD+ + H+

37% enantiomeric excess

2-hexanone + NADH + H+

2-hexanol + NAD+

2-hexanone + NADH + H+

activity is 1.6fold higher than with N-benzyl-3-pyrrolidinone

2-hexanone + NADH + H+

hexan-2-ol + NAD+

50.4fold higher activity compared to activity with N-benzyl-3-pyrrolidinone

2-hexanone + NADPH + H+

hexan-2-ol + NADP+

5.9% of the activity with 2-hydroxyacetophenone and NADPH

2-hydroxyacetophenone + NADH + H+

(S)-1-phenyl-1,2-ethanediol + NAD+

2.4% of the activity with NADPH

2-hydroxyacetophenone + NADPH + H+

(S)-1-phenyl-1,2-ethanediol + NADP+

2-hydroxymethylpyrene + NAD+

2-formylpyrene + NADH + H+

2-mercaptoethanol + NAD+

2-methoxybenzaldehyde + NADH + H+

2-methoxybenzylalcohol + NAD+

13% of the activity with 2,2,2-trifluoroacetophenone

2-methoxybenzyl alcohol + NAD+

2-methoxybenzaldehyde + NADH + H+

25% of the activity with (S)-(-)-1-phenylethanol

2-methoxyethanol + NAD+

2-methoxyacetaldehyde + NADH + H+

Ogataea angusta

H9ZGN0

721388

2-methyl-2,4-pentanediol + NAD+

1% activity compared to cyclohexanol

2-methylbutan-1-ol + NAD+

? + NADH

2-methylbutyraldehyde + NADH + H+

2-methylbutanol + NAD+

1.4% of the activity with acetaldehyde

2-methylcyclohexanol + NAD+

(+)-2-methylcyclohexanone + NADH + H+

2-methylcyclohexanone + NADH + H+

2-methylcyclohexanol + NAD+

13% of the activity compared to 1-phenyl-1,2-propanedione

2-methylcyclohexanone + NADH + H+

? + NAD+

46% activity compared to cyclohexanone

2-methylpropan-1-ol + NAD+

2-methyl-propan-1-one + NADH

2-methylpropan-1-ol + NAD+

2-methyl-propan-1-one + NADH + H+

2-methylpropionaldehyde + NADH + H+

2-methylpropanal + NAD+

3.3% of the activity with acetaldehyde

2-nitrobenzaldehyde + NADH + H+

(2-nitrophenyl)methanol + NAD+

33% of activity with N-benzyl-3-pyrrolidinone

2-nitrobenzaldehyde + NADH + H+

2-nitrobenzyl alcohol + NAD+

low activity

Flavobacterium frigidimaris

2-nonanol + NAD+

2-nonanone + NADH + H+

Q8L3C9

weak activity

668532

2-nonanone + NADH

(S)-2-nonanol + NAD+ + H+

95% enantiomeric excess

2-octanol + NAD+

2-octanone + NADH + H+

2-octanone + NADH

(S)-2-octanol + NAD+ + H+

92% enantiomeric excess

2-octanone + NADH + H+

2-octanol + NAD+

2-octanone + NADPH + H+

octan-2-ol + NADP+

11.9% of the activity with 2-hydroxyacetophenone and NADPH

2-oxo-3-methylpentane + NADH

3-methylpentan-2-ol + NAD+

2-oxo-3-phenylpropane + NADH

2-hydroxy-3-phenylpropane + NAD+

2-oxo-4-methylpentane + NADH

4-methylpentan-2-ol + NAD+

2-oxo-butyric acid + NADH + H+

2-hydroxybutyric acid + NAD+

15% of activity with N-benzyl-3-pyrrolidinone

2-oxohexane + NADH

2-hydroxyhexane + NAD+

2-oxopentane + NADH

2-pentanol + NAD+

2-oxopentanoate + NADH + H+

2-hydroxypentanoate + NAD+

low activity

2-oxopropanal + NADH + H+

? + NAD+

328% of the activity with acetoin

2-pentanol + NAD+

2-pentanone + NADH + H+

7 entries

2-pentanone + NADH + H+

(S)-2-pentanol + NAD+

60% enantiomeric excess

2-pentanone + NADH + H+

2-pentanol + NAD+

2-pentanone + NADH + H+

pentan-2-ol + NAD+

285586

2-pentanone + NADPH + H+

pentan-2-ol + NADP+

11.9% of the activity with 2-hydroxyacetophenone and NADPH

2-phenylcyclohexanone + NADH + H+

? + NAD+

2% activity compared to cyclohexanone

2-phenylethanol + NAD+

2-phenylethanone + NADH + H+

57% activity compared to cyclohexanol

2-phenylethanol + NAD+

phenylacetaldehyde + NADH + H+

2-phenylpropanal + NADH + H+

(S)-2-phenylpropanol + NAD+

18 h, 74% yield, 98% enantiomeric excess

2-phenylpropionaldehyde + NADH + H+

2-phenylpropanol + NAD+

2-propanol + NAD(P)+

acetone + NAD(P)H

2-propanol + NAD+

2-propanone + NADH

2-propanol + NAD+

2-propanone + NADH + H+

2-propanol + NAD+

aceton + NADH + H+

723979

irreversible, no measurable activity with acetone

2-propanol + NAD+

acetone + NADH

655741

2-propanol + NAD+

acetone + NADH + H+

12 entries

2-propanol + NAD+

propanone + NADH + H+

2-[3-(2-phenyl-1,3-dioxolan-2-yl)phenyl]propanal + NADH + H+

(S)-2-(3-(2-phenyl-1,3-dioxolan-2-yl)phenyl)propanol + NAD+

18 h, 95% yield, 61% enantiomeric excess

3',4'-dimethoxyphenylacetone + NADH + H+

1-(3,4-dimethoxyphenyl)propan-2-ol + NAD+

24% of the activity with phenyl trifluoromethyl ketone

3'-bromoacetophenone + NADH + H+

1-(3-bromophenyl)ethanol + NAD+

151% of the activity with phenyl trifluoromethyl ketone

3'-chloroacetophenone + NADH + H+

1-(3-chlorophenyl)ethanol + NAD+

70% of the activity with phenyl trifluoromethyl ketone

3'-methoxyacetophenone + NADH + H+

1-(3-methoxyphenyl)ethanol + NAD+

51% of the activity with phenyl trifluoromethyl ketone

3,3,5-trimethylcyclohexanone + NADH + H+

? + NAD+

2% activity compared to cyclohexanone

3,4-dihydro-retinol + NAD+

3,4-dihydro-retinal

654370

3,4-dimethylbenzyl alcohol + NAD+

3,4-dimethylbenzaldehyde + NADH + H+

3,4-hexanedione + NADH + H+

77% of the activity with N-benzyl-3-pyrrolidinone

3,4-methylenedioxyphenyl acetone + NADH

(S)-(3,4-methylenedioxyphenyl)-2-propanol + NAD+ + H+

Zygosaccharomyces rouxii

658565

3,5-dimethylcyclohexanol + NAD+

3,5-dimethylcyclohexanone + NADH + H+

1% activity compared to cyclohexanol

3-acetylpyridine + NADH + H+

(R)-1-(3-pyridyl)ethanol + NAD+

3-acetylpyridine + NADH + H+

1-pyridin-3-ylethanol + NAD+

7.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone

3-aminobenzyl alcohol + NAD+

3-aminobenzaldehyde + NADH + H+

8% activity compared to cyclohexanol

3-bromobenzyl alcohol + NAD+

3-bromobenzaldehyde + NADH + H+

678116

3-bromobenzylalcohol + NAD+

3-bromobenzaldehyde + NADH + H+

3-butene-1-ol + NAD+

? + NADH

Zea mays

285630

3-chloro-2-butanone + NADH + H+

3-chlorobutan-2-ol + NAD+

151% of the activity with phenyl trifluoromethyl ketone

3-chlorobenzaldehyde + NADH + H+

3-chlorobenzyl alcohol + NAD+

3-heptanol + NAD+

3-heptanone + NADH + H+

93% of the activity with 2-propanol

3-hydroxy-2-butanone + NADH + H+

2,3-butanediol + NAD+

3-hydroxy-2-butanone + NADPH + H+

2,3-butanediol + NADP+

3-methoxy-1-phenylpropan-1-one + NADH

3-methoxy-1-phenylpropan-1-ol + NAD+

3-methoxybenzaldehyde + NADH + H+

3-methoxybenzyl alcohol + NAD+

3-methoxybenzaldehyde + NADH + H+

3-methoxybenzylalcohol + NAD+

14% of the activity with 2,2,2-trifluoroacetophenone

3-methoxybenzyl alcohol + NAD+

3-methoxybenzaldehyde + NADH + H+

3-methoxybenzylalcohol + NAD+

3-methoxybenzaldehyde + NADH + H+

3-methyl-1-butanol + NAD+

? + NADH

285647

3-methyl-2-cyclohexenone + NADH + H+

? + NAD+

1% activity compared to cyclohexanone

3-methylbutan-1-ol + NAD+

3-methyl-butan-1-one + NADH + H+

3-methylbutan-2-ol + NAD+

3-methylbutan-2-one + NADH

R-(-)-3-methylbutan-2-ol and S-(+)-3-methylbutan-2-ol

3-methylbutan-2-one + NADH

3-methylbutan-2-ol + NAD+

3-methylbutan-2-one + NADH + H+

3-methyl-2-butanol + NAD+

3-methylbutan-2-one + NADH + H+

3-methylbutan-2-ol + NAD+

678363

3-methylbutanal + NAD+

? + NADH + H+

3-methylbutanal + NADPH + H+

3-methylbutanol + NADP+

P28625

low activity, reaction of EC 1.1.1.2

763285

3-methylbutanol + NAD+

3-methylbutanone + NADH + H+

133% activity compared to cyclohexanol

3-methylbutanol + NAD+

? + NADH

3-methylbutyraldehyde + NADH + H+

3-methylbutanol + NAD+

2.9% of the activity with acetaldehyde

3-methylcyclohexanol + NAD+

3-methylcyclohexanone + NADH

3-methylcyclohexanol + NAD+

3-methylcyclohexanone + NADH + H+

14% of activity compared to (S)-1-indanol

3-methylcyclohexanol + NAD+

10% of the activity compared to isoborneol

3-methylcyclohexanone + NADH

3-methylcyclohexanol + NAD+

3-methylcyclohexanone + NADH + H+

3-methylcyclohexanol + NAD+

33% of the activity compared to 1-phenyl-1,2-propanedione

3-methylphenylethyl alcohol + NAD+

64% activity compared to cyclohexanol

3-nitroacetophenone + NADH + H+

1-(3-nitrophenyl)ethanol + NAD+

3-nitrobenzaldehyde + NADH + H+

3-nitrobenzyl alcohol + NAD+

3-oxo-5beta-androstan-17beta-ol + NADH

3beta,17beta-dihydroxy-5beta-androstane + NAD+

3-oxoheptane + NADH

3-hydroxyheptane + NAD+

3-pentanol + NAD+

3-pentanone + NADH + H+

3-pentanone + NADH

pentan-3-ol + NAD+

285586

3-pentanone + NADPH + H+

pentan-3-ol + NADP+

7.1% of the activity with 2-hydroxyacetophenone and NADPH

3-penten-2-one + NADH + H+

? + NAD+

2% activity compared to cyclohexanone

3-phenyl-1-propanol + NAD+

3-phenyl-1-propanone + NADH

285568, 285569

3-phenyl-2-propen-1-ol + NAD+

? + NADH

Zea mays

285630

3-phenylpropanol + NAD+

3-phenylpropanal + NADH + H+

135% of the activity with 2-phenylethanol

3-phenylpropionaldehyde + NADH

3-phenylpropan-1-ol + NAD+

1218% of the activity with phenyl trifluoromethyl ketone

3-phenylpropionaldehyde + NADH + H+

3-phenylpropanol + NAD+

6.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone

3-pyridylcarbinol + NAD+

pyridine-3-carbaldehyde + NADH

285573

3beta,12alpha-dihydroxy-5beta-cholanoic acid + NAD+

? + NADH

3beta,17beta-dihydroxy-5beta-androstane + NAD+

5beta-androstan-3,17dione + NADH

285583

3beta,7alpha,12alpha-trihydroxy-5beta-cholanoic acid + NAD+

? + NADH

3beta,7alpha-dihydroxy-5beta-cholanoic acid + NAD+

? + NADH

3beta-7alpha-dihydroxy-5beta-cholanoate + NAD+

17-hydroxy-3-oxo-5beta-cholanoate + NADH + H+

3beta-hxdroxy-5alpha-cholanoate + NAD+

3-oxo-5alpha-cholanoate + NADH

285583

3beta-hydroxy-5beta-androstan-17-one + NAD+

5beta-androstan-3,17-dione + NADH

3beta-hydroxy-5beta-cholanoate + NAD+

3-oxo-5beta-cholanoate + NADH

285583

4'-bromoacetophenone + NADH + H+

1-(4-bromophenyl)ethanol + NAD+

77% of the activity with phenyl trifluoromethyl ketone

4'-chloroacetophenone + NADH + H+

1-(4-chlorophenyl)ethanol + NAD+

60% of the activity with phenyl trifluoromethyl ketone

4'-chlorobutyrophenone + NADH + H+

1-(4-chlorophenyl)butan-1-ol + NAD+

10% of the activity with 2,2,2-trifluoroacetophenone

4-acetylpyridine + NADH + H+

(R)-1-(4-pyridyl)ethanol + NAD+

4-acetylpyridine + NADH + H+

1-pyridin-4-ylethanol + NAD+

64.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone

4-amino-1-butanol + NAD+

to gamma-butyrolactam + NADH + H+

95% conversion

760252

4-bromoacetophenone + NADH + H+

1-(4-bromophenyl)ethanol + NAD+

4-bromobenzyl alcohol + NAD+

4-bromobenzaldehyde + NADH + H+

678116

4-bromobenzylalcohol + NAD+

4-bromobenzaldehyde + NADH + H+

4-carboxybenzaldehyde + NADH + H+

4-carboxybenzyl alcohol + NAD+

678116

4-carboxybenzaldehyde + NADH + H+

4-carboxybenzylalcohol + NAD+

4-chloroacetophenone + NADH

1-(4-chlorophenyl)ethanol + NAD+

4-chloroacetophenone + NADH + H+

1-(4-chlorophenyl)ethanol + NAD+

4-chlorobenzaldehyde + NADH + H+

4-chlorobenzyl alcohol + NAD+

4-deoxy-L-erythro-5-hexoseulose + NAD+

2-dehydro-3-deoxy-D-gluconate + NADH + H+

4-ethylcyclohexanol + NAD+

4-ethylcyclohexanone + NADH + H+

60% activity compared to cyclohexanol

4-ethylcyclohexanone + NADH + H+

? + NAD+

22% activity compared to cyclohexanone

4-fluoroacetophenone + NADH + H+

1-(4-fluorophenyl)ethanol + NAD+

4-hydroxy-2-butanone + NADH + H+

2,4-dihydroxybutane + NAD+

26% of the activity with phenyl trifluoromethyl ketone

4-hydroxy-retinol + NAD+

4-oxo-retinal + NADH

654370

4-hydroxymethylpyrene + NAD+

4-formylpyrene + NADH + H+

4-methoxy-1-naphthaldehyde + NAD+

4-methoxy-1-naphthol + NADH + H+

fluorogenic substrate of class I and II isozymes

722073

4-methoxy-1-naphthaldehyde + NAD+

4-methoxy-1-naphthyl alcohol + NADH + H+

substrate for class I ADH

686331

4-methoxy-1-naphthaldehyde + NADH + H+

4-methoxynaphthalene-1-carbaldehyde + NAD+

substrate for class I ADH

697802

4-methoxybenzaldehyde + NADH + H+

4-methoxybenzyl alcohol + NAD+

51% of the activity with butan-2-ol

4-methoxybenzaldehyde + NADH + H+

4-methoxybenzylalcohol + NAD+

13% of the activity with 2,2,2-trifluoroacetophenone

4-methoxybenzyl alcohol + NAD+

4-methoxybenzaldehyde + NADH + H+

4-methoxybenzylalcohol + NAD+

4-methoxybenzaldehyde + NADH + H+

4-methoxyphenylacetone + NADH

(2S)-1-(4-methoxyphenyl)propan-2-ol + NAD+ + H+

4-methyl-1-pentanol + NAD+

4-methyl-1-pentanal + NADH

4-methylcyclohexanol + NAD+

4-methylcyclohexanone + NADH + H+

56% activity compared to cyclohexanol

4-methylcyclohexanone + NADH + H+

4-methylcyclohexanol + NAD+

60% of the activity compared to 1-phenyl-1,2-propanedione

4-methylcyclohexanone + NADH + H+

? + NAD+

25% activity compared to cyclohexanone

4-methylpentan-2-one + NADH + H+

4-methyl-2-pentanol + NAD+

4-nitrobenzaldehyde + NADH + H+

4-nitrobenzyl alcohol + NAD+

4-nitrobenzaldehyde + NADH + H+

4-nitrobenzylalcohol + NAD+

4-nitrobenzyl alcohol + NAD+

4-nitrobenzaldehyde + NADH + H+

94% activity compared to benzyl alcohol

4-nitrosodimethylaniline + NAD+

? + NADH + H+

photometric assay substrate

722073

4-nitrotetrazolium violet + NADH + H+

a formazan + NAD+

4-phenylbutan-2-one + NADH

4-phenylbutan-2-ol + NAD+

5-acetyl-7-chlorofuro[2,3-c]pyridine + NADH + H+

5-(1-hydroxyethyl)-7-chlorofuro[2,3-c]pyridine + NAD+

5-acetylfuro[2,3-c]pyridine + NADH + H+

5-(1-hydroxyethyl)furo[2,3-c]pyridine + NAD+

5-amino-1-pentanol + NAD+

delta-valerolactam + NADH + H+

38% conversion

760252

5-hydroxymethylfurfural + NADH + H+

(furan-2,5-diyl)dimethanol + NAD+

5-hydroxymethylfurfural + NADH + H+

5-hydroxymethylfurfuryl alcohol + NAD+

P28625

low activity

763285

5alpha-androstan-17beta-ol-3-one + NADH + H+

3beta,17beta-dihydroxy-5alpha-androstan + NAD+

5alpha-pregnan-3beta-ol-20-one + NAD+

5alpha-pregnan-3,20-dione + NADH

low activity

5beta-androstan-17beta-ol-3-one + NAD+

5beta-androstan-3,17-dione + NADH

5beta-androstan-3beta-ol-17-one + NAD+

5beta-androstan-3,17-dione + NADH

5beta-cholanic acid-3-one + NADH

5beta-cholanic acid-3-ol + NAD+

low activity

5beta-cholanic acid-3-one + NADH + H+

5beta-cholanic acid-3-ol + NAD+

5beta-pregnan-21-ol-3,20-dione hemisuccinate + NAD+

5beta-pregnan-3,20,21-trione hemisuccinate + NADH + H+

285597

5beta-pregnan-3,20-dione + NADH

5beta-pregnan-3beta-ol-20-one + NAD+

5beta-pregnan-3,20-dione + NADH

6-amino-1-hexanol + NAD+

epsilon-caprolactam + NADH + H+

Acinetobacter calcoaceticus

14% conversion

760252

6-benzyloxy-3,5-dioxo-hexanoic acid ethyl ester + NADH + H+

(3R,5S)-6-benzyloxy-3,5-dihydroxy-hexanoic acid ethyl ester + NAD+

659674

6-methoxy-2-naphthaldehyde + NADH + H+

(6-methoxynaphthalen-2-yl)methanol + NAD+

7-cis-retinol + NAD+

7-cis-retinal + NADH

9-cis-retinol + NAD+

9-cis-retinal + NADH

a primary alcohol + NAD+

an aldehyde + NADH + H+

Mus musculus

ADH3 is involved in multiple cellular pathways, as diverse as formaldehyde detoxification, retinoid metabolism and NO homeostasis, ADH3 is considered to play only a minor role in hepatic alcohol metabolism because ethanol concentrations rarely exceed 50 mM

697214

acetaldehyde + NADH + H+

ethanol + NAD+

59 entries

acetaldehyde + NADH + H+

ethanol + NAD+ + H+

acetaldehyde + NADPH + H+

ethanol + NADP+

7 entries

acetoin + NAD+

diacetyl + NADH

18% of the activity with 2,3-butanediol

acetoin + NADH + H+

2,3-butanediol + NAD+

acetoin + NADH + H+

3-hydroxy-2-butanol + NAD+

acetoin + NADH + H+

butan-2,3-diol + NAD+

acetone + NADH

isopropanol + NAD+

acetone + NADH + H+

2-propanol + NAD+

acetone + NADH + H+

propan-2-ol + NAD+

acetone + NADPH + H+

propan-2-ol + NADP+

14.7% of the activity with 2-hydroxyacetophenone and NADPH

acetophenone + NADH

(R)-alpha-phenyl ethanol + NAD+

Pseudomonas fluorescens

the enzyme exhibits high stereoselectivity in the desymmetrization of the prochiral ketone acetophenone, producing optically pure (R)-alpha-phenyl ethanol at high conversion

654294

acetophenone + NADH + H+

(R)-1-phenylethanol + NAD+

6% of the activity with phenyl trifluoromethyl ketone, in the reverse reaction 87% of the activity with 2-propanol

99% enantiomeric excess

acetophenone + NADH + H+

(S)-(-)-1-phenylethanol + NAD+

more than 99% enantiomeric excess

acetophenone + NADH + H+

(S)-1-phenylethanol + NAD+

99% enantiomeric excess

acetophenone + NADH + H+

1-phenylethanol + NAD+

15 entries

acetophenone + NADPH + H+

1-phenylethanol + NADP+

5.1% of the activity with 2-hydroxyacetophenone and NADPH

acetylacetone + 2 NADH + 2 H+

2,4-pentanediol + 2 NAD+

acetylacetone + NADH + H+

4-hydroxypentan-2-one + NAD+

1% activity compared to cyclohexanone

1% activity compared to cyclohexanone

acetylacetone + NADH + H+

63% of the activity with N-benzyl-3-pyrrolidinone

acetylpyrazine + NADH + H+

(R)-1-(pyrazyl)ethanol + NAD+

alcohol + NAD+

aldehyde or ketone + NADH

all-trans retinal + NADH + H+

all-trans retinol + NAD+

Kangiella koreensis

C7R702

26.8% of the activity with butan-1-ol

737923

all-trans-retinal + NADH + H+

all-trans-retinol + NAD+

285648

all-trans-retinol + NAD+

all-trans-retinal + NADH

allyl alcohol + NAD+

acrolein + NADH

allyl alcohol + NAD+

prop-2-en-1-al + NADH

Flavobacterium frigidimaris

allylalcohol + NAD+

prop-2-enal + NADH + H+

Q8L3C9

668532

alpha-ethyl benzoylformate + NADH + H+

ethyl (R)-(-)-mandelate + NAD+

95% enantiomeric excess

alpha-methyl benzoylformate + NADH + H+

methyl (R)-mandelate + NAD+

92% enantiomeric excess

alpha-tetralone + NADH + H+

(S)-alpha-tetralol + NAD+

alpha-tetralone + NADH + H+

alpha-tetralol + NAD+

anisaldehyde + NADH + H+

anisic alcohol + NAD+

285621, 285625, 678363

benzacetaldehyde + NADPH + H+

benzol + NADP+

P28625

low activity, reaction of EC 1.1.1.2

763285

benzaldehyde + NADH

benzyl alcohol + NAD+

benzaldehyde + NADH + H+

benzyl alcohol + NAD+

benzaldehyde + NADPH + H+

benzyl alcohol + NADP+

107% of the activity with (2E)-but-2-enal, yield 99% after 12 h

benzil + NADH + H+

(R)-benzoin + NAD+

62% of the activity compared to 1-phenyl-1,2-propanedione. The enzyme catalyses the asymmetric reduction of benzil to (R)-benzoin with both excellent conversion (98%) and optical purity (98%) by way of an efficient in situ NADH-recycling system involving a second thermophilic ADH

benzoin + NADH + H+

1,2-diphenylethane-1,2-diol

13% of activity with N-benzyl-3-pyrrolidinone

benzoylformic acid + NADPH + H+

mandelate + NADP+

2.4% of the activity with 2-hydroxyacetophenone and NADPH

benzyl alcohol + acycloNAD+

benzaldehyde + acycloNADH + H+

acycloNAD+ i.e. NAD+-analogue, where the nicotinamide ribosyl moiety has been replaced by the nicotinamide (2-hydroxyethoxy)methyl moiety

benzyl alcohol + NAD+

benzaldehyde + NADH

10 entries

benzyl alcohol + NAD+

benzaldehyde + NADH + H+

23 entries

benzyl alcohol + NADP+

benzaldehyde + NADPH + H+

benzylacetone + NADH + H+

4-phenylbutan-2-ol + NAD+

benzylalcohol + NAD+

benzaldehyde + NADH + H+

benzyloxycarbonyl-3-pyrrolidinone + NADH + H+

(S)-benzyloxycarbonyl-3-pyrrolidinol + NAD+

production with 99.6% enantiomeric excess

butan-1-ol + acycloNAD+

butanal + acycloNADH + H+

acycloNAD+ i.e. NAD+-analogue, where the nicotinamide ribosyl moiety has been replaced by the nicotinamide (2-hydroxyethoxy)methyl moiety

butan-1-ol + NAD+

butanal + NADH + H+

butan-2,3-diol + NAD+

acetoin + NADH + H+

butan-2-ol + NAD+

butan-2-one + NADH

15 entries

butan-2-ol + NAD+

butanone + NADH + H+

83% of the activity with butan-2-ol

butan-2-one + NADH

butan-2-ol + NAD+

butanal + NADH

1-butanol + NAD+ + H+

% of the activity with phenyl trifluoromethyl ketone

butanal + NADH

n-butanol + NAD+

butanal + NADH + H+

butan-1-ol + NAD+

Mycobacterium sp.

W8VSK8

737865

butanol + NAD+

1-butanal + NADH + H+

Haloferax volcanii

D4GSN2

about 15% of the activity with ethanol or 1-propanol

726769

butanol + NAD+

butanal + NADH

Crocus sativus

654140

butanol + NAD+

butyraldehyde + NADH

22 entries

butanol + NAD+

butyraldehyde + NADH + H+

butanol + NAD+

n-butanal + NADH

654743

butyraldehyde + NAD+

n-butanol + NADH + H+

butyraldehyde + NADH

n-butanol + NAD+

butyraldehyde + NADH + H+

butanol + NAD+

56% of the activity with acetaldehyde

butyraldehyde + NADH + H+

n-butanol + NAD+

butyraldehyde + NADPH + H+

butanol + NADP+

22% of the activity with NADH

capronaldehyde + NADH

1-hexanol + NAD+

chloroacetone + NADH + H+

1-chloro-2-propanol + NAD+

chloroacetone + NADH + H+

1-chloropropan-2-ol + NAD+

choline + NAD+

? + NADH + H+

3% activity compared to benzyl alcohol

cinnamaldehyde + NADH + H+

cinnamyl alcohol + NAD+

cinnamyl alcohol + NAD+

cinnamaldehyde + NADH

cinnamyl alcohol + NAD+

cinnamaldehyde + NADH + H+

723979

cinnamyl alcohol + NAD+

cinnamyl aldehyde + NADH + H+

cis-4-methylcyclohexanol + NAD+

4-methylcyclohexanone + NADH

cis-decahydro-1-naphthol + NAD+

11% of activity compared to (S)-1-indanol

crotyl alcohol + NAD+

crotonaldehyde + NADH

cycloheptanol + NAD+

cycloheptanone + NADH + H+

cycloheptanone + NADH

cycloheptanol + NAD+

cyclohexanol + NAD+

cyclohexanone + NADH

cyclohexanol + NAD+

cyclohexanone + NADH + H+

9 entries

cyclohexanone + NADH

cyclohexanol + NAD+

cyclohexanone + NADH + H+

activity is 2.1fold higher than with N-benzyl-3-pyrrolidinone

cyclohexanone + NADH + H+

cyclohexanol + NAD+

cyclooctanone + NADH

cyclooctanol + NAD+

cyclopentanol + NAD+

? + NADH

cyclopentanol + NAD+

cyclopentanone + NADH + H+

cyclopentanone + NADH

cyclopentanol + NAD+

cyclopentanone + NADH + H+

? + NAD+

1% activity compared to cyclohexanone

cyclopetanone + NADH + H+

cyclopentanol + NAD+

D-allonate + NADH + H+

10% activity compared to 2-dehydro-3-deoxy-D-gluconate

D-altronate + NADH + H+

8% activity compared to 2-dehydro-3-deoxy-D-gluconate

D-arabinose + NADH + H+

D-arabitol + NAD+

66% of the activity with 2,3-butanediol

D-arabitol + NADH + H+

D-lyxose + D-arabinose + NAD+

D-erythrose + NAD+

D-glucitol + NAD+

? + NADH

D-gluconate + NADH + H+

79% activity compared to 2-dehydro-3-deoxy-D-gluconate

D-glyceraldehyde + NAD+

D-mannonate + NADH + H+

37% activity compared to 2-dehydro-3-deoxy-D-gluconate

D-ribonate + NADH + H+

0.3% activity compared to 2-dehydro-3-deoxy-D-gluconate

D-sorbitol + NADH + H+

L-gulose + NAD+

0.01% activity compared to 2-dehydro-3-deoxy-D-gluconate

D-talonate + NADH + H+

3% activity compared to 2-dehydro-3-deoxy-D-gluconate

D-threose + NAD+

D-xylonate + NADH + H+

1% activity compared to 2-dehydro-3-deoxy-D-gluconate

decahydro-2-naphthol + NAD+

37% activity compared to cyclohexanol

decan-1-ol + NAD+

decanal + NADH + H+

decyl aldehyde + NADH + H+

1-decanol + NAD+

21% activity compared to benzyl alcohol

diacetyl + 2 NADH + 2 H+

2,3-butandiol + 2 NAD+

diacetyl + NADH + H+

diacetyl + NADH + H+

acetoin + NAD+

150% of the activity with acetoin

diethylketone + NADH + H+

3-pentanol + NAD+

digitose + NAD+

? + NADH

285571

dihydro-4,4-dimethyl-2,3-furandione + NADH + H+

13.1fold higher activity compared to activity with N-benzyl-3-pyrrolidinone

dihydroxyacetone + NADH + H+

glycerol + NAD+

36% of the activity with acetoin

dihydroxyacetone phosphate + NADH + H+

glycerol phosphate + NAD+

82% of the activity with acetoin

DL-glyceraldehyde + H+

glycerol + NAD+

31% of the activity with acetoin

docosanol + NAD+

? + NADH + H+

immobilized HLAD shows about 60% reaction rate and free HLAD shows about 20% reaction rate with docosanol compared to ethanol at pH 8.8 and 30°C

dodecan-1-ol + NAD+

dodecanal + NADH + H+

dodecanol + NAD+

dodecanal + NADH + H+

immobilized HLAD shows about 60% reaction rate and free HLAD shows about 35% reaction rate with dodecanol compared to ethanol at pH 8.8 and 30°C

eicosan-1-ol + NAD+

n-eicosanal + NADH + H+

ethanol + 3-benzoylpyridine-adenine dinucleotide

acetaldehyde + ?

rapid equilibrium bi bi mechanism

285588

ethanol + acycloNAD+

acetaldehyde + acycloNADH + H+

acycloNAD+ i.e. NAD+-analogue, where the nicotinamide ribosyl moietyhas been replaced by the nicotinamide (2-hydroxyethoxy)methyl moiety

ethanol + beta-NAD+

acetaldehyde + NADH + H+

689887

ethanol + NAD+

acetaldehyde + NADH

75 entries

ethanol + NAD+

acetaldehyde + NADH + H+

69 entries

ethanol + NAD+

aldehyde + NADH + H+

immobilized HLAD shows 100% reaction rate and free HLAD shows about 55% reaction rate with ethanol at pH 8.8 and 30°C

ethanol + NADP+

acetaldehyde + NADPH + H+

ethyl (2,4-dichlorophenyl)(oxo)acetate + NADH + H+

ethyl (2R)-(2,4-dichlorophenyl)(hydroxy)acetate + NAD+

the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters

ethyl (3,5-difluorophenyl)(oxo)acetate + NADH + H+

ethyl (2R)-(3,5-difluorophenyl)(hydroxy)acetate + NAD+

the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters

ethyl (4-bromophenyl)(oxo)acetate + NADH + H+

ethyl (2R)-(4-bromophenyl)(hydroxy)acetate + NAD+

the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters

ethyl (4-chlorophenyl)(oxo)acetate + NADH + H+

ethyl (2R)-(4-chlorophenyl)(hydroxy)acetate + NAD+

the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters

ethyl (4-cyanophenyl)(oxo)acetate + NADH + H+

ethyl (2R)-(4-cyanophenyl)(hydroxy)acetate + NAD+

the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters

ethyl (4-fluorophenyl)(oxo)acetate + NADH + H+

ethyl (2R)-(4-fluorophenyl)(hydroxy)acetate + NAD+

the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters

ethyl (4-methylphenyl)(oxo)acetate + NADH + H+

ethyl (2R)-hydroxy(4-methylphenyl)acetate + NAD+

the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters

ethyl 2-oxopropanoate + NADPH + H+

(R)-ethyl 2-hydroxypropanoate + NADP+

294& of the activity with acetoin

ethyl 3,3-dimethyl-2-oxobutanoate + NADH + H+

ethyl (2R)-2-hydroxy-3,3-dimethylbutanoate + NAD+

enantioselectivity of the aliphatic a-ketoester reduction is moderate with the major product is the (S)-enantiomer, 71% enantiomeric excess of ethyl (2S)-2-hydroxy-3,3-dimethylbutanoate

ethyl 3,3-dimethyl-2-oxobutanoate + NADH + H+

ethyl (2S)-2-hydroxy-3,3-dimethylbutanoate + NAD+

enantioselectivity of the aliphatic a-ketoester reduction is moderate with the major product is the (S)-enantiomer, 71% enantiomeric excess of ethyl (2S)-2-hydroxy-3,3-dimethylbutanoate

ethyl 3-methyl-2-oxobutanoate + NADH + H+

ethyl (2R)-2-hydroxy-3-methylbutanoate + NAD+

enantioselectivity of the aliphatic a-ketoester reduction is moderate with the major product is the (S)-enantiomer, 44% enantiomeric excess of ethyl (2S)-2-hydroxy-3-methylbutanoate

ethyl 3-methyl-2-oxobutanoate + NADH + H+

ethyl (2S)-2-hydroxy-3-methylbutanoate + NAD+

enantioselectivity of the aliphatic a-ketoester reduction is moderate with the major product is the (S)-enantiomer, 44% enantiomeric excess of ethyl (2S)-2-hydroxy-3-methylbutanoate

ethyl 3-methyl-2-oxobutyrate + NADH + H+

1-phenyl-1,2-propanedione and ethyl 3-methyl-2-oxobutyrate are the best substrate in the reduction reaction

ethyl 3-methyl-2-oxobutyrate + NADH + H+

ethyl-2-hydroxy-3-methylbutyrate + NAD+

33% of the activity with phenyl trifluoromethyl ketone

ethyl 3-oxo-3-phenylpropanoate + NADH + H+

ethyl (3S)-3-hydroxy-3-phenylpropanoate + NAD+

ethyl (3S)-3-hydroxy-3-phenylpropanoate is produced with 99% enantiomeric excess

ethyl 3-oxobutanoate + NADH

ethyl 3-hydroxybutanoate + NAD+

ethyl 3-oxobutanoate + NADH + H+

ethyl (3S)-3-hydroxybutanoate + NAD+

ethyl (3S)-3-hydroxybutanoate is produced with 95% enantiomeric excess

ethyl 3-oxobutanoate + NADH + H+

ethyl 3-hydroxybutanoate + NAD+

ethyl 3-oxobutyrate + NADH + H+

ethyl (S)-3-hydroxybutyrate + NAD+

695705

ethyl 3-oxohexanoate + NADH

ethyl 3-hydroxyhexanoate + NAD+

ethyl 3-oxohexanoate + NADH + H+

ethyl (3R)-3-hydroxyhexanoate + NAD+

ethyl (3R)-3-hydroxyhexanoate is produced with 24% enantiomeric excess

ethyl 3-oxopentanoate + NADH

ethyl 3-hydroxypentanoate + NAD+

ethyl 3-oxopentanoate + NADH + H+

ethyl (3S)-3-hydroxypentanoate + NAD+

ethyl (3S)-3-hydroxypentanoate + NAD+is produced with 60% enantiomeric excess

ethyl 3-phenyl-3-oxopropanoate + NADH

ethyl 3-phenyl-3-hydroxypropanoate + NAD+

ethyl 4-bromo-3-oxobutanoate + NADH + H+

ethyl 4-bromo-3-hydroxybutanoate

511% of the activity with phenyl trifluoromethyl ketone

ethyl 4-chloro-3-oxobutanoate + NADH + H+

(S)-ethyl-4-chloro-3-hydroxybutanoate + NAD+

ethyl 4-chloro-3-oxobutanoate + NADH + H+

ethyl (3R)-4-chloro-3-hydroxybutanoate + NAD+

ethyl (3R)-4-chloro-3-hydroxybutanoate is produced with 4% enantiomeric excess

ethyl 4-chloro-3-oxobutanoate + NADH + H+

ethyl 4-chloro-3-hydroxybutanoate + NAD+

809% of the activity with phenyl trifluoromethyl ketone

ethyl 4-chloroacetoacetate + NADH + H+

6.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone

ethyl 4-chloroacetoacetate + NADH + H+

ethyl 4-chloro-3-hydroxybutanoate + NAD+

ethyl 4-chloroacetoacetate + NADPH

activity is 1.48fold higher than with 2-hydroxyacetophenone and NADPH

ethyl 4-methyl-3-oxopentanoate + NADH + H+

ethyl (3S)-3-hydroxy-4-methylpentanoate + NAD+

ethyl (3S)-3-hydroxy-4-methylpentanoate is produced with 16% enantiomeric excess

ethyl acetoacetate + NADH + H+

42.4fold higher activity compared to activity with N-benzyl-3-pyrrolidinone

ethyl acetoacetate + NADH + H+

ethyl 3-hydroxybutanoate + NAD+

ethyl benzoylformate + NADH

about 30% of the activity compared to isatin

ethyl benzoylformate + NADH

ethyl (R)-mandelate + NAD+

enantiomeric excess of 99.9%

ethyl benzoylformate + NADH + H+

23% of the activity compared to 1-phenyl-1,2-propanedione

ethyl benzoylformate + NADH + H+

ethyl (R)-(-)-mandelate + NAD+

95% enantiomeric excess

ethyl benzoylformate + NADH + H+

ethyl (R)-mandelate + NAD+

ethyl oxo(phenyl)acetate + NADH + H+

ethyl (2R)-hydroxy(phenyl)acetate + NAD+

the enzyme catalyzes the reduction of aromatic alpha-ketoesters to the corresponding enantiomerically pure (R)-configured alpha-hydroxyesters

ethyl oxo(phenyl)acetate + NADH + H+

ethyl hydroxy(phenyl)acetate + NAD+

100% of the activity with 2,2,2-trifluoroacetophenone

ethyl pyruvate + NADH

ethyl pyruvate + NADH

ethyl 2-hydroxypropanoate + NAD+

ethyl pyruvate + NADH + H+

ethyl 2-hydroxypropanoate + NAD+

488% of the activity with phenyl trifluoromethyl ketone

ethyl pyruvate + NADH + H+

ethyl 2-hydroxypropionate + NAD+

16% of the activity compared to 1-phenyl-1,2-propanedione

ethyl pyruvate + NADH + H+

ethyl lactate + NAD+

ethyl trifluoroacetoacetate + NADPH + H+

1-ethoxy-2,2,2-trifluoroethanol + NADP+

4.3% of the activity with 2-hydroxyacetophenone and NADPH

ethylene glycol + NAD+

ethylene glycol + NAD+

? + NADH

ethylenglycol + NAD+

? + NADH

formaldehyde + NADH + H+

methanol + NAD+

7 entries

furfural + NADH + H+

furfuryl alcohol + NAD+

furfuryl alcohol + NAD+

furfural + NADH

glutaraldehyde + NADH + H+

70.8fold higher activity compared to activity with N-benzyl-3-pyrrolidinone

glutaraldehyde + NADH + H+

? + NAD+

best substrate in reduction reaction

glycerol + NAD+

dihydroxyacetone + NADH + H+

glycolaldehyde + NADH + H+

glycol + NAD+

heptaldehyde + NADH + H+

? + NAD+

33% activity compared to benzyl alcohol

heptan-2-ol + NAD+

heptan-2-one + NADH

heptan-4-ol + NAD+

heptan-4-one + NADH

hexadecan-1-ol + NAD+

hexadecanal + NADH + H+

hexadecanol + NAD+

hexadecanal + NADH

Oncorhynchus mykiss

very low activity

285614

hexadecanol + NAD+

hexadecanal + NADH + H+

immobilized HLAD shows about 60% reaction rate and free HLAD shows about 15% reaction rate with hexadecanol compared to ethanol at pH 8.8 and 30°C

hexaldehyde + NADH + H+

1-hexanol + NAD+

7% activity compared to benzyl alcohol

hexan-1-ol + acycloNAD+

hexanal + acycloNADH + H+

acycloNAD+ i.e. NAD+-analogue, where the nicotinamide ribosyl moiety has been replaced by the nicotinamide (2-hydroxyethoxy)methyl moiety

hexan-1-ol + NAD+

hexanal + NADH + H+

hexan-1-ol + NAD+

n-hexanal + NADH

hexan-2-ol + NAD+

2-hexanone + NADH

hexanal + NADH + H+

1-hexanol + NAD+

69.6% of the activity with glutaraldehyde

hexanal + NADH + H+

hexan-1-ol + NAD+

16% of the activity with acetoin

hexanal + NADH + H+

n-hexanol + NAD+

hexanal + NADPH + H+

1-hexanone + NADP+

24% of the activity with NADH

hexanol + NAD+

hexaldehyde + NADH

Mus musculus

655479

hexanol + NAD+

hexanal + NADH

655479

hexanol + NAD+

n-hexanal + NADH

hydrocinnamaldehyde + NADH + H+

hydrocinnamyl alcohol + NAD+

12% activity compared to benzyl alcohol

hydrocinnamyl alcohol + NAD+

hydrocinnamaldehyde + NADH + H+

31% activity compared to benzyl alcohol

hydroxyacetone + NADH + H+

propane-1,2-diol + NAD+

27% of the activity with N-benzyl-3-pyrrolidinone

isatin + NADH

isatin + NADH + H+

? + NAD+

iso-propanol + NAD+

? + NADH + H+

762802

iso-propanol + NAD+

isopropanal + NADH + H+

isoamylalcohol + NAD+

3-methylbutanal + NADH + H+

isoborneol + NAD+

isoborneol is the best substrate in the oxidation reaction

isobutanal + NADH + H+

isobutanol + NAD+

20% of the activity with glutaraldehyde

isobutyl alcohol + NAD+

? + NADH

285575

isobutyraldehyde + NADH + H+

isobutanol + NAD+

isobutyramide + NAD+

656058

isopentan-1-ol + NAD+

isopentanal + NADH + H+

Arabidopsis thaliana

34% of the activity with ethanol

739588

isopentanol + NAD+

? + NADH + H+

isopentenyl alcohol + NAD+

isopentanone + NADH

285575

isopropanol + NAD+

2-propanone + NADH + H+

isopropanol + NAD+

acetone + NADH + H+

isopropanol + NAD+

propan-2-one + NADH + H+

L-glyceraldehyde + NAD+

levulinic acid + NADH + H+

11% of activity with N-benzyl-3-pyrrolidinone

m-chlorophenacyl chloride + NADH + H+

2-chloro-1-(3-chlorophenyl)ethanol + NAD+

4.6fold higher activity compared to activity with N-benzyl-3-pyrrolidinone

m-nitrobenzaldehyde + NAD+

m-nitrobenzyl alcohol + NADH + H+

substrate of class IV ADH

686331

m-nitrobenzaldehyde + NADH + H+

m-nitrobenzyl alcohol + NAD+

mannitol + NAD+

? + NADH + H+

methanol + NAD+

formaldehyde + NADH + H+

37 entries

methyl 3-oxobutanoate + NADH + H+

methyl 3-hydroxybutanoate + NAD+

methyl 4-bromo-3-oxobutanoate + NADH + H+

methyl 4-bromo-3-hydroxybutanoate + NAD+

164% of the activity with phenyl trifluoromethyl ketone

methyl 4-chloro-3-oxobutanoate + NADH + H+

methyl 4-chloro-3-hydroxybutanoate + NAD+

methyl acetoacetate + NADH + H+

methyl 3-hydroxybutanoate + NAD+

methyl benzoylformate + NADH

methyl benzoylformate + NADH + H+

methyl (R)-(-)-mandelate + NAD+

92% enantiomeric excess

methyl benzoylformate + NADH + H+

methyl (S)-mandelate + NAD+

6 h, 100% conversion, 17% enantiomeric excess

methyl glyoxal + NADH + H+

3.5fold higher activity compared to activity with N-benzyl-3-pyrrolidinone

methyl o-chlorobenzoylformate + NADH

about 55% of the activity compared to isatin

methyl o-chlorobenzoylformate + NADH + H+

methyl (R)-o-chloromandelate + NAD+

6 h, 99% conversion, 72% enantiomeric excess

methyl oxo(phenyl)acetate + NADH + H+

methyl hydroxy(phenyl)acetate + NAD+

57% of the activity with 2,2,2-trifluoroacetophenone

methyl pyruvate + NADH + H+

methyl 2-hydroxypropanoate + NAD+

18.9fold higher activity compared to activity with N-benzyl-3-pyrrolidinone

methyl pyruvate + NADH + H+

methyl lactate + NAD+

methylbenzoylformate + NADH + H+

13% of the activity with 2,2,2-trifluoroacetophenone

methylcrotonyl alcohol + NAD+

methylcrotonaldehyde + NADH

Mesocricetus auratus

285650

methylglyoxal + NADH + H+

? + NAD+

Petroselinum crispum

11.9% of the activity with acetaldehyde

285627

methylglyoxal + NADH + H+

acetol + NAD+

Candida albicans

A0A1D8PP43

742509

N-benzyl-3-piperidone + NADH + H+

N-benzylpiperidin-3-ol + NAD+

2.3fold higher activity compared to activity with N-benzyl-3-pyrrolidinone

N-benzyl-3-pyrrolidinol + NAD+

1-benzylpyrrolidin-3-one + NADH + H+

33% of the activity with (S)-N-benzyl-3-pyrrolidinol

N-benzyl-3-pyrrolidinone + NADH + H+

(R)-N-benzyl-3-pyrrolidinol + NAD+

(R)-stereoselectivity of the reduction carried out with the heat-treated cells

N-benzyl-3-pyrrolidinone + NADH + H+

(S)-N-benzyl-3-pyrrolidinol + NAD+

N-benzyl-4-piperidone + NADH + H+

N-benzylpiperidin-4-ol + NAD+

10.7fold higher activity compared to activity with N-benzyl-3-pyrrolidinone

n-butanal + NADH + H+

n-butanol + NAD+

n-butanol + NAD+

butanal + NADH

Thermomicrobium roseum

low activity

655262

n-butanol + NAD+

butylaldehyde + NADH + H+

n-butanol + NAD+

butyraldehyde + NADH

655741

n-butanol + NAD+

butyraldehyde + NADH + H+

n-butanol + NAD+

n-butanal + NADH

n-butanol + NAD+

n-butanal + NADH + H+

n-butanol + NADH + H+

butyraldehyde + NAD+

n-butylaldehyde + NADH + H+

butanal + NAD+

activity is 1.8fold higher than with N-benzyl-3-pyrrolidinone

n-butyraldehyde + NADH + H+

n-butanol + NAD+

7.3fold higher activity compared to activity with N-benzyl-3-pyrrolidinone

n-decanol + NAD+

n-decanal + NADH

n-hexanol + NAD+

n-hexanal + NADH

n-hexylaldehyde + NADH + H+

hexanal + NAD+

activity is 1.6fold higher than with N-benzyl-3-pyrrolidinone

n-octylaldehyde + NADH + H+

n-octanol + NAD+

activity is 1.7fold higher than with N-benzyl-3-pyrrolidinone

n-pentanol + NAD+

pentanal + NADH + H+

n-pentylaldehyde + NADH + H+

n-pentanol + NAD+

Zymomonas mobilis

285623

n-propanol + NAD+

n-propanal + NADH

n-propanol + NAD+

propanal + NADH

n-propanol + NAD+

propanal + NADH + H+

n-propanol + NAD+

propionaldehyde + NADH

Thermomicrobium roseum

655262

N-tert-butoxycarbonyl-3-pyrrolidinone + NADH + H+

(S)-N-tert-butoxycarbonyl-3-pyrrolidinol + NAD+

production of (S)-N-tert-butoxycarbonyl-3-pyrrolidinol with 99.6% enantiomeric excess

n-valeraldehyde + NADH + H+

n-pentanol + NAD+

activity is 2.8fold higher than with N-benzyl-3-pyrrolidinone

N-[4-(chloroacetyl)-2-nitrophenyl]acetamide + NADH + H+

N-[4-[(1R)-2-chloro-1-hydroxyethyl]-2-nitrophenyl]acetamide + NAD+

AE010289.1

production of N-[4-[(1R)-2-chloro-1-hydroxyethyl]-2-nitrophenyl]acetamide with 98% enantiomeric excess

nonyl aldehyde + NADH + H+

1-nonanol + NAD+

25% activity compared to benzyl alcohol

nonylphenol polyethoxylate 9 + NAD+

? + NADH

7% activity compared to benzyl alcohol

octacosan-1-ol + NAD+

octacosanal + NADH + H+

octadecan-1-ol + NAD+

octadecanal + NADH + H+

octan-1-ol + NAD+

n-octanal + NADH

10 entries

octan-1-ol + NAD+

octanal + NADH + H+

octan-2-ol + NAD+

octan-2-one + NADH

octanal + NADH + H+

octanol + NAD+

octanol + NAD+

octanal + NADH

octanol + NAD+

octanal + NADH + H+

octyl aldehyde + NADH + H+

1-octanol + NAD+

29% activity compared to benzyl alcohol

octylphenol polyethoxylate 2 + NAD+

? + NADH

59% activity compared to benzyl alcohol

octylphenol polyethoxylate 8 + NAD+

? + NADH

7% activity compared to benzyl alcohol

oxalacetic acid + NADH + H+

14% of activity with N-benzyl-3-pyrrolidinone

oxaloacetate + NADH + H+

? + NAD+

p-chlorobenzaldehyde + NADH + H+

p-chlorobenzyl alcohol + NAD+

1.2fold higher activity compared to activity with N-benzyl-3-pyrrolidinone

p-methoxybenzyl alcohol + NAD+

p-methoxybenzaldehyde + NADH + H+

p-nitrobenzaldehyde + NADH

p-nitrobenzyl alcohol + NAD+

Mus musculus

285603

p-nitrobenzaldehyde + NADH + H+

p-nitrobenzyl alcohol + NAD+

substrate of isozyme ADH4

691867

p-nitrobenzyloxycarbonyl-3-pyrrolidinone + NADH + H+

(S)-N-p-nitrobenzyloxycarbonyl-3-pyrrolidinol + NAD+

production with 90.7% enantiomeric excess

p-nitrophenyl octanoate + H2O

p-nitrophenol + octanoate

acid-assisted nucleophilic catalysis involving the ammonium ion of Lys and the thiolate of Cys in the acyl-oxygen cleavage

285594

pentan-1-ol + NAD+

pentanal + NADH + H+

pentan-2-ol + NAD+

2-pentanone + NADH

pentan-2-ol + NAD+

pentan-2-one + NADH + H+

pentan-3-ol + NAD+

3-pentanone + NADH

pentan-3-ol + NAD+

3-pentanone + NADH + H+

pentanal + NAD+

pentanone + NADH

pentanal + NADH

1-pentanol + NAD+ + H+

132% of the activity with phenyl trifluoromethyl ketone, in the reverse reaction 5% of the activity with 2-propanol

pentanal + NADH + H+

1-pentanol + NAD+

38% of the activity with glutaraldehyde

pentanol + NAD+

n-pentanal + NADH

12 entries

pentanol + NAD+

pentanal + NADH + H+

pentanol + NAD+

valeraldehyde + NADH + H+

240% activity compared to cyclohexanone

phenyl trifluoromethyl ketone + NADH + H+

(S)-1-phenyltrifluoroethanol + NAD+

more than 99% enantiomeric excess

phenylacetaldehyde + NADH

phenylethanol + NAD+

phenylalaninol + NAD+

? + NADH

285571

phenylethanol + NAD+

phenylacetaldehyde + NADH

R-(+)-phenylethanol and S-(-)-phenylethanol

phenylethanol + NAD+

phenylacetaldehyde + NADH + H+

phenylglyoxylic acid + NADH + H+

hydroxy(phenyl)acetic acid + NAD+

enzyme covalently immobilized to magnetic Fe3O4 nanoparticles via glutaraldehyde retains 48.77% activity of its original activity

687134

phytol + NAD+

phytenal + NADH + H+

721749

polyoxyethylene 8 decyl ether + NAD+

? + NADH

12% activity compared to benzyl alcohol

primary or secondary alcohol + NAD+

aldehyde or ketone + NADH

prop-2-en-1-ol + NAD+

? + NADH

Camellia sinensis

140.6% of the activity with ethanol

285628

prop-2-en-1-ol + NAD+

prop-2-enal + NADH + H+

85% of the activity with butan-2-ol

propan-1,2-diol + NAD+

? + NADH

propan-1-ol + acycloNAD+

propanal + acycloNADH + H+

acycloNAD+ i.e. NAD+-analogue, where the nicotinamide ribosyl moiety has been replaced by the nicotinamide (2-hydroxyethoxy)methyl moiety

propan-1-ol + NAD+

propanal + NADH + H+

propan-2-ol + NAD+

acetone + NADH

18 entries

propan-2-ol + NAD+

acetone + NADH + H+

propanal + NADH + H+

propan-1-ol + NAD+

38% of the activity with butan-2-ol

propanal + NADH + H+

propanol + NAD+

739927

propanol + NAD+

propionaldehyde + NADH + H+

18 entries

propionaldehyde + NADH

propanol + NAD+

propionaldehyde + NADH + H+

1-propanol + NAD+

propionaldehyde + NADH + H+

activity is 1.1fold higher than with N-benzyl-3-pyrrolidinone

propiophenone + NADH + H+

1-phenylpropan-1-ol + NAD+

27% of activity with N-benzyl-3-pyrrolidinone

pyridine 2-aldehyde + NADH + H+

? + NAD+

pyridine 3-aldehyde + NADH + H+

? + NAD+

pyridine 4-aldehyde + NADH + H+

? + NAD+

pyridine-3-aldehyde + NADH + H+

pyridin-3-yl ethanol + NAD+

82% of activity with N-benzyl-3-pyrrolidinone

pyridine-4-aldehyde + NADH + H+

pyridin-4-yl methanol + NAD+

4.4fold higher activity compared to activity with N-benzyl-3-pyrrolidinone

pyruvaldehyde + NADH + H+

lactaldehyde + NAD+

81% of the activity with acetoin

R-(+)-1-phenylethanol + NAD+

1-phenylethanone + NADH

R-(+)-cis-verbenol + NAD+

? + NADH

R-(+)-trans-bicyclo(2.2.1)-heptanol + NAD+

R-(+)-trans-bicyclo(2.2.1)-heptanal + NADH

R-(+)-trans-bicyclo(2.2.1)-heptanol and S-(-)-trans-bicyclo(2.2.1)-heptanol

rac-3-methylcyclohexanone + NADH + H+

(1S,3S)-3-methylcyclohexanol + NAD+

684246

retinal + NADH + H+

retinol + NAD+

substrate of isozyme ADH4

691867

retinol + NAD+

retinal + NADH

655197

S-(-)-1-phenylethanol + NAD+

1-phenylethanone + NADH

S-(-)-cis-bicyclo(2.2.1)-heptanol + NAD+

S-(-)-cis-bicyclo(2.2.1)-heptanal + NADH

S-(-)-trans-bicyclo(2.2.1)-heptanol + NAD+

S-(-)-cis-bicyclo(2.2.1)-heptanal + NADH + H+

1054% activity compared to ethanol

secondary alcohol + NAD+

aldehyde + NADH

Synechocystis sp. PCC 6803

656475

sinapaldehyde + NADH

sinapyl alcohol + NAD+

P74721

preferred substrates

760374

sinapaldehyde + NADH + H+

sinalcohol + NAD+

Synechocystis sp. PCC 6803

sinapaldehyde + NADH + H+

sinapyl alcohol + NAD+

P74721

preferred substrates

760374

tert-butanol + NAD+

butyraldehyde + NADH + H+

762802

tert-butyl 3-oxobutanoate + NADH + H+

tert butyl 3-hydroxybutanoate + NAD+

568% of the activity with phenyl trifluoromethyl ketone

tert-butyl acetoacetate + NADH + H+

tetracosanol + NAD+

? + NADH + H+

immobilized HLAD shows about 55% reaction rate and free HLAD shows about 15% reaction rate with tetracosanol compared to ethanol at pH 8.8 and 30°C

tetracosanol-1-ol + NAD+

tetracosanal + NADH + H+

tetradecan-1-ol + NAD+

tetradecanal + NADH + H+

tetrahydro-4H-pyran-4-one + NADH + H+

? + NAD+

10% activity compared to cyclohexanone

tetralin-1-ol + NAD+

3,4-dihydronaphthalen-1(2H)-one + NADH + H+

51% of the activity compared to isoborneol

trans-4-(N,N-dimethylamino)-cinnamaldehyde + NADH

trans-4-(N,N-dimethylamino)-cinnamyl alcohol + NAD+

285574

trans-4-methylcyclohexanol + NAD+

4-methylcyclohexanone + NADH

trans-cinnamaldehyde + NADH + H+

cinnamyl alcohol + NAD+

trans-cinnamyl alcohol + NAD+

(2E)-3-phenylprop-2-enal + NADH + H+

activity is 3.9fold higher than with (S)-(-)-1-phenylethanol

triacontan-1-ol + NAD+

triacontanal + NADH + H+

Tris + NAD+

? + NADH

Triton X-100 + NAD+

? + NADH

7% activity compared to benzyl alcohol

Triton X-114 + NAD+

? + NADH

3% activity compared to benzyl alcohol

Triton X-165 + NAD+

? + NADH

24% activity compared to benzyl alcohol

Triton X-35 + NAD+

? + NADH

43% activity compared to benzyl alcohol

Triton X-405 + NAD+

? + NADH

19% activity compared to benzyl alcohol

Triton X-45 + NAD+

? + NADH

7% activity compared to benzyl alcohol

tryptophol + NAD+

? + NADH

285569

ubiquinone + NADH + H+

ubiquinol + NAD+

valeraldehyde + NADH

1-pentanol + NAD+

vanillyl alcohol + NAD+

vanillin + NADH