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1.1.1.1: alcohol dehydrogenase

This is an abbreviated version!
For detailed information about alcohol dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.1

Reaction

a primary alcohol
+
NAD+
=
an aldehyde
 +
NADH
 +
H+

Synonyms

(R)-specific alcohol dehydrogenase, 40 kDa allergen, Aadh1, acetaldehyde-alcohol dehydrogenase, ADH, ADH 1, ADH class III, ADH I, ADH II, ADH-10, ADH-A, ADH-A2, ADH-B2, ADH-C2, ADH-HT, ADH-I, ADH1, ADH1B, ADH1C, ADH1C*1, ADH1C*2, Adh1p, ADH2, ADH3, ADH4, ADH5, ADH6Hp, ADH8, AdhA, AdhB, AdhC, AdhD, AdhE, ADHES77, ADS1, AFPDH, alcohol dehydrogenase, alcohol dehydrogenase (NAD), alcohol dehydrogenase 1, alcohol dehydrogenase 10, alcohol dehydrogenase 2, alcohol dehydrogenase 3, alcohol dehydrogenase 5, alcohol dehydrogenase class-P, alcohol dehydrogenase D, alcohol dehydrogenase GroES domain protein, alcohol dehydrogenase I, alcohol dehydrogenase II, Alcohol dehydrogenase-B2, alcohol dependent dehydrogenase, alcohol-aldehyde/ketone oxidoreductase, NAD+-dependent, alcohol:NAD+ oxidoreductase, aldehyde dehydrogenase, aldehyde reductase, aldehyde/alcohol dehydrogenase, ALDH, aliphatic alcohol dehydrogenase, alpha-ketoaldehyde dehydrogenase, anti-Prelog reductase, APE2239, APE_2239.1, ARAD1B16786p, bi-functional alcohol/aldehyde dehydrogenase, bifunctional acetaldehyde-alcohol dehydrogenase, bifunctional alcohol/aldehyde dehydrogenase, CHY1186, class I ADH, class I ALDH, class II ADH, class III ADH, class III alcohol dehydrogenase, class IV ADH, Cm-ADH2, Cthe_0423, DADH, dehydrogenase, alcohol, ethanol dehydrogenase, FALDH, FDH, Gastric alcohol dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase, glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase, GSH-FDH, GSH-FDH/ADH, HLAD, hLADH, HpADH3, HtADH, HvADH1, HVO_2428, iron-containing alcohol dehydrogenase, KlADH4, KlDH3, KmADH3, KmADH4, LSADH, medium chain alcohol dehydrogenase, medium-chain NAD+-dependent ADH, medium-chain secondary alcohol dehydrogenase, MGD, More, NAD(H)-dependent alcohol dehydrogenase, NAD+-ADH, NAD+-dependent (S)-stereospecific alcohol dehydrogenase, NAD+-dependent alcohol dehydrogenase, NAD+-dependent SDR, NAD+-linked alcohol dehydrogenase 1, NAD+-linked methylglyoxal dehydrogenase, NAD-dependent alcohol dehydrogenase, NAD-dependent medium-chain ADH, NAD-specific aromatic alcohol dehydrogenase, NADH-alcohol dehydrogenase, NADH-aldehyde dehydrogenase, NADH-dependent alcohol dehydrogenase, NADH-dependent anti-Prelog specific ADH, NADH:p-NTF-reductase, Octanol dehydrogenase, Pcal_1311, PF0991 protein, PF1960, PFADH, primary alcohol dehydrogenase, Retinol dehydrogenase, SaADH, SaADH2, Saci_1232, SADH, SCAD, sec-ADH A, short-chain ADH, short-chain dehydrogenase/reductase, short-chain NAD(H)-dependent dehydrogenase/reductase, slr1192, SSADH, SsADH-10, SSO2536, ST0053, Ta1316 ADH, TaDH, TBADH, Teth39_0206, Teth39_0218, Teth514_0627, TK0845, Tsac_0416, Y-ADH, YADH, YADH-1, yeast alcohol dehydrogenase, YIM1, YLL056C, YMR152W, Ymr152wp

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.1 alcohol dehydrogenase

Specific Activity

Specific Activity on EC 1.1.1.1 - alcohol dehydrogenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.02
-
mutant recombinant enzyme E97C, from crude extract, at 65°C
0.05
wild-type, cosubstrate NADPH, pH 7.0, 55°C
0.07
gene AdhE deletion mutant, cosubstrate NADPH, pH 7.0, 55°C
0.092
-
partially purified enzyme
0.1
-
isoenzyme 4
0.12
-
isoenzyme 1
0.18
after 5.67fold purification, using benzylalcohol as substrate, at 65°C
0.22
crude extract, at pH 10.0
0.25
gene AdhE deletion mutant, cosubstrate NADPH, pH 7.0, 55°C
0.3
-
substrates 1,3-propanediol and NAD+, pH 9.0, 60°C, recombinant enzyme, with NiCl2 addition
0.44
-
substrate formaldehyde, pH 7.0, 30°C
0.47
-
substrate furfural, pH 7.0, 30°C
0.5
purified mutant enzyme W95L, using benzyl alcohol as substrate, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.64
-
wild type enzyme, crude extract, at 65°C
0.65
-
-
0.68
wild-type, cosubstrate NADPH, pH 7.0, 55°C
0.74
pH 7.0, 30°C, substrate 3.methylbutanal with NADPH
0.75
purified enzyme, using benzylalcohol as substrate, at 80°C
0.78
-
crude extract, at pH 10.0
1.1
-
substrates ethanol and NAD+, pH 9.0, 60°C, recombinant enzyme
1.12
pH 7.0, 30°C, substrate benzaldehyde with NADPH
1.28
-
purified enzyme
1.36
-
isoenzyme A2
1.47
-
-
1.52
wild-type, cosubstrate NADH, pH 7.0, 55°C
1.74
pH 7.0, 30°C, substrate 5-hydroxymethylfurfural with NADH
10.1
-
after 12.9fold purification, at pH 10.0
108
substrate butan-2,3-diol, maximal specific activity detected in oxidation reaction, 70°C, pH 8.8
11.4
recombinant enzyme, crude Escherichia coli cell extract, substrate ethyl 4-chloro-3-oxobutanoate, pH 7.0, 30°C
15
crude enzyme, after heat treatment at 75°C for 20 min
17.6
crude extract Ta1316 ADH with ethanol as a substrate, at pH 5.0 and 75°C
18.6
purified mutant enzyme W95L/N249Y, using benzyl alcohol as substrate, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
190.86
pH 7.0, 30°C, substrate acetaldehyde with NADH
196.3
Sporotrichum pulverulentum
-
-
2 - 2.5
substrate diacetyl-acetoin, maximal specific activity detected in reduction reaction, 70°C, pH 6.1
2.1
-
isoenzyme AA-ADH
2.6
in Tris-HCl buffer (pH 7.6), 5 mM dithiothreitol, 0.2 mM NADH, and 10 mM acetaldehyde at 65°C
2.88
after 12.9fold purification, at pH 10.0
20.6
after 1.4fold purifictaion
26.1
-
mutant enzyme N249Y, at 65°C
27.6
-
purified native enzyme, pH 6.5, 30°C
3.2
-
reduction of 3-oxo-5beta-androstan-17beta-ol
31.8
Megalodesulfovibrio gigas
-
-
32.5
-
ADH-1
361
-
purified ADH II
4.03
-
recombinant wild type enzyme, after 6fold purification, at 65°C
4.1
-
wild type enzyme, at 65°C
4.3
recombinant enzyme, crude Escherichia coli cell extract, substrate ethyl 4-chloro-3-oxobutanoate, pH 7.0, 30°C
4.5
-
wild type recombinant enzyme, after 450fold purification, at 65°C
4.75
-
purified recombinant enzyme, pH 6.5, 50°C
42.7
purified recombinant His-tagged wild-type enzyme, pH 7.5, temperature not specified in the publication, aldehyde formation
447.6
substrate ethanol, pH 8, 60°C
470
-
enzyme form ADHII
5.05
pH 7.0, 30°C, substrate furfural with NADH
5.18
pH 6.7, 30°C, mutant enzyme S109P/L116S/Y294C
5.2
-
mutant recombinant enzyme E97C, after 260fold purification, at 65°C
5.7
-
recombinant enzyme, crude Escherichia coli cell extract, substrate ethyl 4-chloro-3-oxobutanoate, pH 7.0, 30°C
597
-
purified ADH I
6.3
recombinant enzyme, crude Escherichia coli cell extract, substrate ethyl 4-chloro-3-oxobutanoate, pH 7.0, 30°C
6.5
-
substrate glycolaldehyde, pH 7.0, 30°C
628.7
purified Ta1316 ADH with ethanol as a substrate, at pH 5.0 and 75°C
64
-
isoenzyme C2
65
-
mutant enzyme Adh1-1S1108
66
-
recombinant enzyme, crude Escherichia coli cell extract, substrate ethyl 4-chloro-3-oxobutanoate, pH 7.0, 30°C
7.68
wild-type, cosubstrate NADH, pH 7.0, 55°C
70
-
wild-type enzyme Adh1-1S
70.8
purified recombinant His-tagged mutant C243S, pH 7.5, temperature not specified in the publication, aldehyde formation
79.6
substrate butan-1-ol, pH 6.5, 60°C
8.08
-
isoenzyme B2
9.64
pH 7.0, 30°C, substrate glycolaldehyde with NADH
9.9
recombinant enzyme, crude Escherichia coli cell extract, substrate ethyl 4-chloro-3-oxobutanoate, pH 7.0, 30°C
93
-
enzyme form ADHI
additional information